Western Blot (WB) (All lanes: Creatine kinase M-type antibody at 2ug/ml+293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 42 kDaObserved band size: 42 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embeded mouse heart tissue using MBS7006401 at dilution of 1:100)
Western Blot (WB) (Western BlotPositive WB detected in:Mouse kidney tissueAll lanes: GSTM3 antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 27 kDaObserved band size: 27 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis tissue using MBS7048329 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of Hela cells using MBS7048329 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunofluorescence (IF) (MBS9604971 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB) (Western blot analysis of extracts from mouse brain, using AIM2 Antibody. Lane 1 was treated with the antigen-specific peptide.)
Western Blot (WB) (Western blotAll lanes: Phosphatidylcholine-sterol acyltransferase antibody at 2ug/mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48kDaObserved band size: 48kDa)
Western Blot (WB) (All lanes: Suppressor of fused homolog antibody at 4ug/mlLane 1:HepG2 whole cell lysateLane 2:Hela whole cell lysateLane 3:mouse kidney tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 54,48,53 kDaObserved band size: 54 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testicle using MBS1496631 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver using MBS1496631 at dilution 1:100)
Western Blot (WB) (Western BlotPositive WB detected in: HEK293 whole cell lysate,HepG2 whole cell lysateAll lanes: MAN2A1 antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 132 KDaObserved band size: 150 KDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung cancer using MBS7052761 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human cervical cancer using MBS7052761 at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells using MBS7052761 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blotAll lanes: PAH antibody at 2.5ug/mlLane 1: Mouse kidney tissueLane 2: Mouse liver tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 52 kDaObserved band size: 52 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7001186 at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells using MBS7001186 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) ( All lanes : Anti-ROR1 Antibody at 1:4000 dilutionLane 1: K562 whole cell lysateLane 2: -MB-231 whole cell lysateLane 3: NCI-H226 whole cell lysateLane 4: A549 whole cell lysateLane 5: NIH/3T3 whole cell lysate Lane 6: Mouse kidney tissue lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 104 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) ( Anti-ROR1 Antibody at 1:4000 dilution + PANC-1 whole cell lysate Lysates/proteins at 20ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 104 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of AP7671d on paraffin-embedded Human heart tissue.Tissue was fixed with formaldehyde at room temperature.Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0).Samples were incubated with primary antibody(1:100) for 1 hour at room temperature.Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of MBS9200685 on paraffin-embedded Human kidney tissue.Tissue was fixed with formaldehyde at room temperature.Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0).Samples were incubated with primary antibody(1:100) for 1 hour at room temperature.Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
Flow Cytometry (FC) ( Overlay histogram showing A549 cells stained with MBS9200685 (green line).The cells were fixedwith 2% paraformaldehyde (10 min).The cells were then icubated in 2% bovine serumalbumin to block non-specific protein-protein interactions followed by the antibody (MBS9200685, 1:25 dilution) for 60 min at 37ºC.The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC.Isotype control antibody (blue line) was rabbit IgG1(1ug/1x10^6 cells) used under the same conditions.Acquisition of >10, 000 events was performed.)
Immunohistochemistry (IHC) ( MBS9200685 staining ROR1 in human kidney tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin-embedded sections).Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature;antigen retrieval was by heat mediation with a citrate buffer (pH6).Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC) ( MBS9200685 staining ROR1 in Human heart tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections).Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature;antigen retrieval was by heat mediation with a citrate buffer (pH6).Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC) ( Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the ROR1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry;clinical relevance has not been evaluated.)
Western Blot (WB) (Western blot analysis of Beta-2 Adrenergic Receptor expression in HepG2 (A), mouse kidney (B), rat kidney (C) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of Beta-2 Adrenergic Receptor staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human gastric cancer using MBS1492657 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney tissue using MBS719800 at dilution of 1:100)
Western Blot (WB) (Western blotAll lanes: Corticosteroid-binding globulin antibody at 2ug/ml+rat liver tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 45kDaObserved band size: 45kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver tissue using MBS7000651 at dilution of 1:100)
Western Blot (WB) (All lanes: 60S ribosomal protein L5 polyclonal Antibody at 2ug/ml+EC109 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 32.7 kDaObserved band size: 32.7 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embeded human colorectal carcinoma using MBS7004722 at diluton of 1:50)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer tissue using MBS7049575 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer tissue using MBS7049575 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of Hela cells using MBS7049575 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blotAll lanes: KYNUantibody at 1.44ug/mlLane 1 : A549 whole cell lysateLane 2 : HepG-2 whole cell lysateLane 3 : Hela whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 53,35 kDaObserved band size: 52 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver tissue using MBS7046365 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7046365 at dilution 1:100)
Immunoprecipitation (IP) (Immunoprecipitating KYNU in HeLa whole cell lysateLane 1: Rabbit monoclonal IgG(1ug) instead of MBS7046365 in HeLa whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS7046365(4ug)+ HeLa whole cell lysate(500ug)Lane 3: HeLa whole cell lysate (20ug))
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embeded human kidney using MBS7001003 at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells using MBS7001003 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blotAll lanes: PLA2G7 antibody at 9ug/mlLane 1: Raw264.7 whole cell lysateLane 2: Mouse liver tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 50 kDaObserved band size: 50 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS1488927 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS1488927 at dilution 1:100)
Western Blot (WB) (All lanes: E3 ubiquitin-protein ligase CBL-C antibody at 14ug/mlLane 1:A549 whole cell lysateLane 2:Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 53,48 kDaObserved band size: 52 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS1491737 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human pancreas tissue using MBS1491737 at dilution 1:100)
Western Blot (WB) (Western blot analysis of DNA Polymerase gamma 2 expression in A549 (A), HeLa (B), NS-1 (C), H9C2 (D) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of DNA Polymerase gamma 2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Western Blot (WB) (Western blotAll lanes: LHCGR antibody at 2ug/mlLane 1:mouse brain tissueLane 2:mouse kidney tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 79,72 kDaObserved band size: 79 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human thyroid tissue using MBS7005330 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis using MBS7005330 at dilution 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells using MBS7005330 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western BlotPositive WB detected in:HEK293 whole cell lysate,Mouse liver tissueAll lanes: DLL4 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 75 kDaObserved band size: 75 kDa)
Immunofluorescence (IF) (Immunofluorescent analysis of MCf-7 cells using MBS7048029 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blotAll lanes: Histone H3.3 antibody at 2ug/ml+Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 15kDaObserved band size: 15kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver cancer using MBS1488722 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human melanoma cancer using MBS1488722 at dilution of 1:100)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using AR Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 40s.)
Western Blot (WB) (Western blotAll lanes: Casein kinase I isoform epsilon antibody at 2ug/mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 45.8 kDaObserved band size: 75kDa)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using PYCARD antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells using PYCARD antibody.)
Western Blot (WB) (Western blotAll lanes: SARS protein antibody at 2ug/mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 56 kDaObserved band size: 56kDaAdditional bands at: 36 kDa.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7005416 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human melanoma cancer using MBS7005416 at dilution of 1:100)
Western Blot (WB) (All lanes: Autophagy-related protein 16-1 antibody at 2ug/ml+MCF-7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 69,67,54,48,50 kDaObserved band size: 68 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta using MBS1497386 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human spleen using MBS1497386 at dilution 1:100)
Western Blot (WB) (Western blotAll lanes: Caspase-8 antibody at 2ug/mlLane 1:ACCM whole cell lysateLane 2:LO2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 56,54,46,58,28,26,33,31,62 kDaObserved band size: 55 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney using MBS7000938 at dilution 1:20)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS7000938 at dilution 1:20)
Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells using MBS7000938 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunofluorescence (IF) (Immunofluorescent analysis of hepg2 using MBS7000938 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western BlotPositive WB detected in: 293 whole cell lysateAll lanes: RPA2 antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 30,31,39 KDaObserved band size: 30 KDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embeded using human colorectal carcinoma using MBS7002702 at dilution 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of Hela cells using MBS7002702 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunoprecipitation (IP) (Immunoprecipitating RPA2 in Hela whole cell lysate Lane 1: Rabbit control IgG (1ug)instead of MBS7002702 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7002702 (6ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
Western Blot (WB) (Western BlotPositive WB detected in:Mouse stomach tissueAll lanes: SLC35A2 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42,26,45,35 kDaObserved band size: 42 kDa)
Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells using MBS7049156 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (All lanes: Interferon alpha/beta receptor 2 antibody at 10ug/ml+MCF7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 58,38,28 kDaObserved band size: 58 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human endometrial cancer using MBS1497080 at dilution 1:100)
Western Blot (WB) (Western BlotPositive WB detected in:Mouse lung tissueAll lanes: PSMB5 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 29,22,18 kDaObserved band size: 29 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS7048337 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver cancer using MBS7048337 at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells using MBS7048337 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human adrenal gland using MBS1490740 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis using MBS1490740 at dilution 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of PC3 cells using MBS1490740 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (All lanes: Actin-related protein 2 antibody at 3ug/mlLane 1:mouse spleen tissueLane 2:Jurkat whole cell lysateLane 3:MCF7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 45,46 kDaObserved band size: 45 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human tonsil using MBS1495599 at dilution 1:100)
Western Blot (WB) (Western BlotPositive WB detected in: A2780 whole cell lysateAll lanes: PAX3 antibody at 5.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53,25,23,45,46,54,56 KDaObserved band size: 53 KDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human skin using MBS7006465 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung cancer using MBS7006465 at dilution 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells using MBS7006465 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))