Western Blot (WB)
(Western Blot: Sample: Human HL60 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human WASP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Wiskott Aldrich Syndrome Protein Polyclonal Antibody | anti-WASP antibody
Wiskott Aldrich Syndrome Protein (WASP) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Human HL60 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human WASP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human HL60 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human WASP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type hl60 cell lysate; Lane 2: WASP knockout hl60 cell lysate; Predicted MW: 53kd Observed MW: 60kd Primary Ab: 1ug/ml Rabbit Anti-Human WASP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type hl60 cell lysate; Lane 2: WASP knockout hl60 cell lysate; Predicted MW: 53kd Observed MW: 60kd Primary Ab: 1ug/ml Rabbit Anti-Human WASP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq, Jul 2008]
Uniprot Description
WASP: a member of the Wiskott-Aldrich syndrome (WAS) family of proteins. A cytoplasmic protein expressed exclusively in hematopoietic cells. Transduces signals from surface receptors to the actin cytoskeleton. Associates with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Mutated in Wiskott-Aldrich syndrome, a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia.
Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: Xp11.23
Cellular Component: actin cytoskeleton; actin filament; cytosol; extracellular exosome; intercellular junction; vesicle membrane
Molecular Function: actin binding; GTPase regulator activity; identical protein binding; phospholipase binding; protein binding; protein kinase binding; Rac GTPase binding; SH3 domain binding; small GTPase binding
Biological Process: actin filament polymerization; actin filament-based movement; actin polymerization or depolymerization; blood coagulation; Cdc42 protein signal transduction; defense response; endosomal transport; epidermis development; Fc-gamma receptor signaling pathway involved in phagocytosis; immune response; negative regulation of cell motility; negative regulation of stress fiber formation; positive regulation of Arp2/3 complex-mediated actin nucleation; protein complex assembly; regulation of actin polymerization and/or depolymerization; regulation of catalytic activity; regulation of lamellipodium assembly; regulation of stress fiber assembly; regulation of T cell antigen processing and presentation; Rho protein signal transduction; T cell activation; T cell receptor signaling pathway
Disease: Neutropenia, Severe Congenital, X-linked; Thrombocytopenia 1
Research Articles on WASP
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Product Notes
The WASP was (Catalog #AAA2032173) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Wiskott Aldrich Syndrome Protein (WASP) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Wiskott Aldrich Syndrome Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the WASP was for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wiskott Aldrich Syndrome Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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