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Western Blot (WB)
(Western Blot: Sample: Lane1: Human A431 Cells; Lane2: Rat Skin Tissue.)
Rabbit anti-Human Calmodulin Like Protein 3 Polyclonal Antibody | anti-CALML3 antibody
Calmodulin Like Protein 3 (CALML3) Polyclonal Antibody
Gene Names
CALML3; CLP
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Calmodulin Like Protein 3; Polyclonal Antibody; Calmodulin Like Protein 3 (CALML3) Polyclonal Antibody; CLP; Calmodulin-related protein NB-1; CaM-like protein; anti-CALML3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CALML3. It has been selected for its ability to recognize CALML3 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
149
Applicable Applications for anti-CALML3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human, Rat
Organism Species
Human
Fragment
CALML3 (Met1~Lys149)
Quality Control
Content: The quality control contains recombinant CALML3 (Met1~Lys149) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2077396
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human A431 Cells; Lane2: Rat Skin Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human A431 Cells; Lane2: Rat Skin Tissue.)
Western Blot (WB)
(Western Blot A: Sample: RecombinantCALML3,Human.)
Western Blot (WB)
(Western Blot A: Sample: RecombinantCALML3,Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Esophagus Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Esophagus Cancer Tissue)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: CALML3 knockout A431 cell lysate; Predicted MW: 16kd Observed MW: 16kd Primary Ab: 1ug/ml Rabbit Anti-Human CALML3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: CALML3 knockout A431 cell lysate; Predicted MW: 16kd Observed MW: 16kd Primary Ab: 1ug/ml Rabbit Anti-Human CALML3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Product Categories/Family for anti-CALML3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,891 Da
NCBI Official Full Name
calmodulin-like protein 3
NCBI Official Synonym Full Names
calmodulin like 3
NCBI Official Symbol
CALML3
NCBI Official Synonym Symbols
CLP
NCBI Protein Information
calmodulin-like protein 3
UniProt Protein Name
Calmodulin-like protein 3
Protein Family
UniProt Gene Name
CALML3
UniProt Synonym Gene Names
CLP
Uniprot Description
CALML3: May be similar to that of authentic calmodulin and may actually compete with calmodulin by binding, with different affinities, to cellular substrates. Belongs to the calmodulin family.
Protein type: Calcium-binding
Chromosomal Location of Human Ortholog: 10p15.1
Molecular Function: calcium ion binding; protein binding
Research Articles on CALML3
Similar Products
Product Notes
The CALML3 calml3 (Catalog #AAA2032678) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Calmodulin Like Protein 3 (CALML3) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Calmodulin Like Protein 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CALML3 calml3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calmodulin Like Protein 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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