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140 results for "Goat Anti Rabbit IgG heavy chain specific " - showing 100-140
Western Blot (WB)
(Western blot analysis of S100A9 expression in human tonsil lysate (MBS179299).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A9 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for S100A9 )
S100A9, Monoclonal Antibody (Cat# AAA179299)
Full Name
Anti-S100A9 Rabbit Monoclonal Antibody
Gene Names
S100A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
Reactivity
Human
Applications
IF, IHC, ICC
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Clathrin heavy chain expression in Hela cell lysate (MBS179940).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLTC monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CLTC )
Clathrin heavy chain, Monoclonal Antibody (Cat# AAA179940)
Full Name
Anti-Clathrin heavy chain Rabbit Monoclonal Antibody
Gene Names
CLTC; Hc; CHC; CHC17; CLH-17; CLTCL2
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CYBB expression in MCF-7 cell lysate (MBS179267).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYBB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYBB )
CYBB/Nox2, Monoclonal Antibody (Cat# AAA179267)
Full Name
Anti-CYBB/Nox2 Rabbit Monoclonal Antibody
Gene Names
CYBB; CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of MYH11 expression in Human testis lysate (MBS179847).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYH11 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MYH11 )
MYH11, Monoclonal Antibody (Cat# AAA179847)
Full Name
Anti-MYH11 Rabbit Monoclonal Antibody
Gene Names
MYH11; AAT4; FAA4; SMHC; SMMHC
Reactivity
Human
Applications
IHC, ICC
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of IgA expression in human plasma lysate (MBS1750097).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGHA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGHA1 )
IgA, Monoclonal Antibody (Cat# AAA1750097)
Full Name
Anti-IgA Rabbit Monoclonal Antibody
Gene Names
IGHA1; IgA1
Reactivity
Human
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Myosin heavy chain expression in mouse heart lysate (MBS179895).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYH6 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MYH6 )
Myosin heavy chain, Monoclonal Antibody (Cat# AAA179895)
Full Name
Anti-Myosin heavy chain Rabbit Monoclonal Antibody
Gene Names
MYH6; ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of NOX2 using anti-NOX2 antibody (MBS1750372).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human U-87MG cell lysate,Lane 2: human Hela cell lysate,Lane 3: human HepG2 cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NOX2 at approximately 65KD. The expected band size for NOX2 is at 65KD. )
NOX2/gp91phox, Polyclonal Antibody (Cat# AAA1750372)
Full Name
Anti-NOX2/gp91phox antibody
Gene Names
CYBB; CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Factor V using anti-Factor V antibody (MBS1750406).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human plasma lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor V antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Factor V at approximately 252KD. The expected band size for Factor V is at 252KD. )
Factor V, Polyclonal Antibody (Cat# AAA1750406)
Full Name
Anti-Factor V Antibody
Gene Names
F5; FVL; PCCF; THPH2; RPRGL1
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Protein C using anti-Protein C antibody (MBS1750707).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein C antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Protein C at approximately 52KD. The expected band size for Protein C is at 52KD. )
Protein C, Polyclonal Antibody (Cat# AAA1750707)
Full Name
Anti-Protein C Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Annexin A2 expression in HeLa cell lysate (MBS179478).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANXA2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ANXA2 )
Annexin A2, Monoclonal Antibody (Cat# AAA179478)
Full Name
Anti-Annexin A2 Rabbit Monoclonal Antibody
Gene Names
ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human stomach carcinoma, using IgG4 Antibody (MBS1750120)IGHG4 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IGHG4 Antibody (MBS1750120)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
IgG4, Monoclonal Antibody (Cat# AAA1750120)
Full Name
Anti-IgG4 Rabbit Monoclonal Antibody
Reactivity
Human
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Ferritin expression in Jurkat cell lysate (MBS179842).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FTH1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FTH1 )
Ferritin, Monoclonal Antibody (Cat# AAA179842)
Full Name
Anti-Ferritin Rabbit Monoclonal Antibody
Gene Names
FTH1; FHC; FTH; HFE5; PLIF; FTHL6; PIG15
Reactivity
Human, Mouse, Rat
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human pancreas, using GRP78 BiP Antibody(MBS179500)HSPA5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSPA5 Antibody (MBS179500)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
GRP78 BiP, Monoclonal Antibody (Cat# AAA179500)
Full Name
Anti-GRP78 BiP Rabbit Monoclonal Antibody
Gene Names
HSPA5; BIP; MIF2; GRP78; HEL-S-89n
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of IGHG3 using anti-IGHG3 antibody (M08374).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGHG3 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGHG3. )
IgG3, Monoclonal Antibody (Cat# AAA1752392)
Full Name
Anti-Human IgG3 Monoclonal Antibody
Gene Names
IGHG3; IgG3
Reactivity
Human
Applications
IP, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Integrin alpha 3 using anti-Integrin alpha 3 antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 whole cell lysate,Lane 2: human PC-3 whole cell lysate,Lane 3: human A431 whole cell lysate,Lane 4: human placenta tissue lysates,Lane 5: human SHG-44 whole cell lysate,Lane 6: human U20S whole cell lysate,Lane 7: human Hela whole cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha 3 antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Integrin alpha 3 at approximately 130KD. The expected band size for Integrin alpha 3 is at 116KD.)
Integrin alpha 3/ITGA3, Polyclonal Antibody (Cat# AAA1751439)
Full Name
Anti-Integrin alpha 3/ITGA3 Antibody
Gene Names
ITGA3; VL3A; CD49C; FRP-2; GAPB3; ILNEB; MSK18; VCA-2; VLA3a; GAP-B3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human ovarian, using Cathepsin D Antibody(MBS179641)CTSD was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTSD Antibody (MBS179641)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Cathepsin D, Monoclonal Antibody (Cat# AAA179641)
Full Name
Anti-Cathepsin D Rabbit Monoclonal Antibody
Gene Names
CTSD; CPSD; CLN10; HEL-S-130P
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human testis, using GRP78 BiP Antibody(MBS179501)HSPA5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSPA5 Antibody (MBS179501)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
GRP78 BiP, Monoclonal Antibody (Cat# AAA179501)
Full Name
Anti-GRP78 BiP Rabbit Monoclonal Antibody
Gene Names
HSPA5; BIP; MIF2; GRP78; HEL-S-89n
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of GCLC expression in HepG2 cell lysate (MBS179726).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLC monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GCLC )
GCLC, Monoclonal Antibody (Cat# AAA179726)
Full Name
Anti-GCLC Rabbit Monoclonal Antibody
Gene Names
GCLC; GCL; GCS; GLCL; GLCLC
Reactivity
Human, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Plasminogen using anti-Plasminogen antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,Lane 2: human HL-60 whole cell lysate,Lane 3: human 22RV1 whole cell lysate,Lane 4: human 293T tissue lysates,Lane 5: human PANC-1 whole cell lysate,Lane 6: human SGC-7901 whole cell lysate,Lane 7: human Caco-2 whole cell lysate,Lane 8: human PC-3 whole cell lysate,Lane 9: rat brain tissue lysates,Lane 10: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plasminogen antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Plasminogen at approximately 100KD. The expected band size for Plasminogen is at 90KD.)
Plasminogen/PLG, Polyclonal Antibody (Cat# AAA1751460)
Full Name
Anti-Plasminogen/PLG Antibody
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Immunogen Affinity Purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of IGLV1-51 using anti-IGLV1-51 antibody (M13347).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGLV1-51 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGLV1-51. )
Lambda Light chain, Monoclonal Antibody (Cat# AAA1752399)
Full Name
Anti-Lambda Light chain Monoclonal Antibody
Reactivity
Human
Applications
FC/FACS, ICC, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunochemical staining of human CD98 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
CD98, Polyclonal Antibody (Cat# AAA8106279)
Full Name
Anti-CD98 Antibody, Rabbit Polyclonal
Gene Names
SLC3A2; 4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC
Reactivity
Human
Applications
WB, EIA, IHC-P, ICC, IF, IP
Purity
Protein A & Antigen Affinity
Pricing
$190/0.05 mL | $240/0.1 mL | $280/0.2 mL | $1,110/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of non-muscle Myosin IIB/MYH10 using anti-non-muscle Myosin IIB/MYH10 antibody (MBS1753544).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysatesLane 3: mouse NIH/3T3 whole cell lysatesLane 4: human SKOV3 whole cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-non-muscle Myosin IIB/MYH10 antigen affinity purified polyclonal antibody (Catalog # MBS1753544) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for non-muscle Myosin IIB/MYH10 at approximately 229KD. The expected band size for non-muscle Myosin IIB/MYH10 is at 229KD. )
non-muscle Myosin IIB/MYH10, Polyclonal Antibody (Cat# AAA1753544)
Full Name
Anti-non-muscle Myosin IIB/MYH10 Antibody
Gene Names
MYH10; NMMHCB; NMMHC-IIB
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of IGHD using anti-IGHD antibody (M05183).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGHD antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGHD. )
IGHD, Monoclonal Antibody (Cat# AAA1752352)
Full Name
Anti-IGHD Monoclonal Antibody
Reactivity
Human
Applications
IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
scb, Polyclonal Antibody (Cat# AAA7182243)
Full Name
Rabbit anti-Drosophila melanogaster (Fruit fly) scb Polyclonal Antibody
Gene Names
scb; alpha-PS3; alphaInt3; alphaPS3; alpha[[PS3]]; aPS3; beta[[PS3]; CG8095; CT21280; CT36929; Dm0620; DmelCG8095; E(Sev-CycE)E93; E(sev-cycE)[e93]; ItgaPS3; l(2)01288; PS3; vol; Vol
Reactivity
Drosophila melanogaster (Fruit fly)
Applications
EIA, Western Blot, ensure identification of antigen
Purity
Protein A/G
Western Blot (WB)
(Western Blot (WB) analysis of Annexin 2 (N137) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:MCF-7 cell lysateLane3:Raw264. 7 cell lysateLane4:H9C2 cell lysate)
Annexin 2, Polyclonal Antibody (Cat# AAA3003923)
Full Name
Annexin 2 (N137) Polyclonal Antibody
Gene Names
ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Pricing
$275/0.05 mL | $365/0.1 mL | $1,375/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of DNTT using anti-DNTT antibody (M05546).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNTT antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DNTT. )
TDT, Monoclonal Antibody (Cat# AAA1752362)
Full Name
Anti-TDT Monoclonal Antibody
Gene Names
DNTT; TDT
Reactivity
Human
Applications
IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunochemical staining of human CTSB in human liver with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
Cathepsin B, Polyclonal Antibody (Cat# AAA8102970)
Full Name
Anti-Cathepsin B Antibody, Rabbit Polyclonal
Gene Names
CTSB; APPS; CPSB
Reactivity
Human
Applications
WB, IHC-P, IP
Purity
Protein A & Antigen Affinity
Pricing
$190/0.05 mL | $240/0.1 mL | $280/0.2 mL | $1,110/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of NOX2/gp91phox/CYBB using anti-NOX2/gp91phox/CYBB antibody (MBS1753313).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human U937 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-NOX2/gp91phox/CYBB antigen affinity purified polyclonal antibody (Catalog # MBS1753313) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for NOX2/gp91phox/CYBB at approximately 65KD. The expected band size for NOX2/gp91phox/CYBB is at 65KD. )
NOX2/gp91phox/CYBB, Polyclonal Antibody (Cat# AAA1753313)
Full Name
Anti-NOX2/gp91phox/CYBB Antibody
Gene Names
CYBB; CGD; NOX2; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Immunochemical staining of human SLC3A2 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
CD98, Polyclonal Antibody (Cat# AAA8106278)
Full Name
Anti-CD98 Antibody, Rabbit Polyclonal
Gene Names
SLC3A2; 4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC
Reactivity
Human
Applications
EIA, IHC-P, ICC, IF
Purity
Protein A & Antigen Affinity
Pricing
$190/0.05 mL | $240/0.1 mL | $280/0.2 mL | $1,110/5x0.2 mL
mew, Polyclonal Antibody (Cat# AAA7191880)
Full Name
Rabbit anti-Drosophila melanogaster (Fruit fly) mew Polyclonal Antibody
Gene Names
mew; alpha-PS1; alpha1Int; alphaPS1; alpha[[PS1]]; aPS1; CG1771; CT5254; DmelCG1771; ItgaPS1; l(1)G0429; l(1)G0443; PS1; PS1alpha; PSalpha1
Reactivity
Drosophila melanogaster (Fruit fly)
Applications
EIA, Western Blot, ensure identification of antigen
Purity
Protein A/G
Western Blot (WB)
(Figure 1. Western blot analysis of Integrin alpha V/ITGAV using anti-Integrin alpha V/ITGAV antibody (MBS1753440).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 whole cell lysatesLane 2: human A431 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha V/ITGAV antigen affinity purified polyclonal antibody (Catalog # MBS1753440) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Integrin alpha V/ITGAV at approximately 130-140KD. The expected band size for Integrin alpha V/ITGAV is at 130KD. )
Integrin alpha V/ITGAV, Polyclonal Antibody (Cat# AAA1753440)
Full Name
Anti-Integrin alpha V/ITGAV Antibody
Gene Names
ITGAV; CD51; MSK8; VNRA; VTNR
Reactivity
Human
Applications
WB, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
if, Polyclonal Antibody (Cat# AAA7151869)
Full Name
Rabbit anti-Drosophila melanogaster (Fruit fly) if Polyclonal Antibody
Gene Names
if; alpha-PS2; alpha2Int; alphaPS2; alphaPS2 integrin; alphaPS2C; alphaPS2m8; alpha[[PS2(ms8)]]; alpha[[PS2]]; aPS2; CG9623; CT27194; DmelCG9623; If; in; inf; ItgaPS2; PS 2; PS2; PS2(c); PS2(m8); PS2(m8)alpha; PS2alpha; PSalpha2
Reactivity
Drosophila melanogaster (Fruit fly)
Applications
EIA, Western Blot, ensure identification of antigen
Purity
Protein A/G
Immunohistochemistry (IHC)
(Immunochemical staining of mouse SLC3A2 in mouse kidney with rabbit polyclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to nephric tubule. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
CD98, Polyclonal Antibody (Cat# AAA8112103)
Full Name
Anti-CD98 Antibody, Rabbit Polyclonal
Gene Names
Slc3a2; 4F2; Cd98; Ly10; Mdu1; 4F2HC; Ly-10; NACAE; Ly-m10; Mgp-2hc; AI314110
Reactivity
Mouse
Applications
WB, EIA, IHC-P
Purity
Protein A & Antigen Affinity
Pricing
$190/0.05 mL | $240/0.1 mL | $280/0.2 mL | $1,110/5x0.2 mL
Testing Data
(Fig.1. Protein was extracted from the non-denaturing Lysis Buffer, then it was verified by Co-IP. While the whole cell lysates (Input) and Co-IP samples were validated with Stat1 monoclonal antibody, Stat2 polyclonal antibody, and GAPDH monoclonal antibody respectively by WB.)
Universal IP/Co-IP Tool (Agarose), Purification Kit (Cat# AAA9719230)
Full Name
Universal IP/Co-IP Tool kit (Agarose)
Applications
IP
Pricing
$230/20 Tests | $1,040/5x20 Tests
Western Blot (WB)
(Anti-CTSB rabbit polyclonal antibody at 1:500 dilution Lane A: A549 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HEK293 Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:38 kDa Observed band size:40 kDa (We are unsure as to the identity of these extra bands.))
Cathepsin B, Polyclonal Antibody (Cat# AAA8102972)
Full Name
Anti-Cathepsin B Antibody, Rabbit Polyclonal
Gene Names
CTSB; APPS; CPSB
Reactivity
Human
Applications
WB, EIA, IHC-P
Purity
Protein A & Antigen Affinity
Pricing
$190/0.05 mL | $240/0.1 mL | $280/0.2 mL | $1,110/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Clathrin heavy chain/CLTC using anti-Clathrin heavy chain/CLTC antibody (MBS1753574).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human Raji whole cell lysatesLane 3: rat brain tissue lysatesLane 4: rat kidney tissue lysatesLane 5: mouse brain tissue lysatesLane 6: mouse kidney tissue lysatesLane 7: mouse ANA-1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Clathrin heavy chain/CLTC antigen affinity purified polyclonal antibody (Catalog # MBS1753574) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Clathrin heavy chain/CLTC at approximately 192KD. The expected band size for Clathrin heavy chain/CLTC is at 192KD. )
Clathrin heavy chain/CLTC, Polyclonal Antibody (Cat# AAA1753574)
Full Name
Anti-Clathrin heavy chain/CLTC Antibody
Gene Names
CLTC; Hc; CHC; CHC17; CLH-17; CLTCL2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed HEK293 cells with anti-SUV420H2 antibody RAB-C409 (MBS488830) Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RAB-C409 (MBS488831) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom MBS488831, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
SUV420H2, Monoclonal Antibody (Cat# AAA488830)
Full Name
Anti-SUV420H2 [RAB-C409]
Reactivity
Human
Applications
EIA, IP-MS, ChIP
Purity
Purified antibody. Purified by Immobilized Metal Affinity Chromatography
Pricing
$635/0.1 mg | $2,660/5x0.1 mg
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-SETD7 antibody RAB-C220 (MBS488824). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of RAB-C220 (MBS488825, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
SETD7, Monoclonal Antibody (Cat# AAA488824)
Full Name
Anti-SETD7 [RAB-C220]
Gene Names
SETD7; KMT7; SET7; SET9; SET7/9
Reactivity
Human
Applications
EIA, IF, IP, IP-MS, ChIP, FC/FACS
Purity
Purified antibody. Purified by Immobilized Metal Affinity Chromatography
Pricing
$635/0.1 mg | $2,660/5x0.1 mg
Testing Data
(Fig.1. Protein was extracted from the non-denaturing Lysis Buffer, then it was verified by Co-IP. While the whole cell lysates (Input) and Co-IP samples were validated with Stat1 monoclonal antibody, Stat2 polyclonal antibody, and GAPDH monoclonal antibody respectively by WB.)
Universal IP/Co-IP Tool (Magnetic Beads), Purification Kit (Cat# AAA9719229)
Full Name
Universal IP/Co-IP Tool kit (Magnetic Beads)
Applications
IP
Pricing
$230/20 Tests | $1,040/5x20 Tests
Immunofluorescence (IF)
(Immunofluorescence staining of fixed HeLa cells with anti-FOXP4 antibody RAB-S40 (MBS488832) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RAB-S40 (MBS488833) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom MBS488833, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
FOXP4, Monoclonal Antibody (Cat# AAA488832)
Full Name
Anti-FOXP4 [RAB-S40]
Gene Names
FOXP4; hFKHLA
Reactivity
Human
Applications
EIA, IP, IF, FC/FACS
Purity
Purified antibody. Purified by Immobilized Metal Affinity Chromatography
Pricing
$635/0.1 mg | $2,660/5x0.1 mg
