$380/7 mL (RTU) | $360/0.1 mL (Concentrate) | $585/0.5 mL (Concentrate) | $890/1 mL (Concentrate) | $470/0.2 mL (Concentrate) | $550/15 mL (RTU) | $195/1 mL (RTU) | $205/0.04 mL (Concentrate) | $3,745/5x1 mL (Concentrate)
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Western blot (WB) (Western blot analysis of Albumin in mouse liver tissue lysate with Albumin antibody at (A) 1 and (B) 2 ug/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of Albumin in mouse liver tissue with Albumin antibody at 5 ug/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of Albumin in human liver tissue with Albumin antibody at 2.5 ug/mL.)
Immunofluorescence (IF) (Immunofluorescence of Albumin in mouse liver tissue with Albumin antibody at 20 ug/ml.)
Immunofluorescence (IF) (Immunofluorescence of Albumin in mouse liver tissue with Albumin antibody at 20 ug/ml.Red: Albumin AntibodyBlue: DAPI staining)
Immunofluorescence (IF) (Immunofluorescence of Albumin in human liver tissue with Albumin antibody at 20 ug/ml.)
Western blot (WB) (Western blot analysis of Albumin expression in human liver tissue lysate with Albumin antibody at 0.25 ug/ml.)
Western Blot (WB) ((0.1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC) (Anti-Nephrin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunofluorescence (IF) (Immunofluorescence of Nephrin in Mouse Kidney cells with Nephrin antibody at 10 ug/ml.)
Western Blot (WB) (Western blot of Nephrin in mouse kidney tissue lysate with Nephrin antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.)
Western Blot (WB) (Figure 1 Western Blot Validation of PD-L1 in HeLa CellsLoading: 15ug of lysates per lane. Antibodies: MBS151123 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Western Blot (WB) (Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse cell linesLoading: 15 ug of lysates per lane. Antibodies: MBS151123 (2ug/mL), MBS154572 (2 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit and or anti-mouse IgG HRP conjugate at 1:10000 and 1:5000 dilution, respectively.)
Western Blot (WB) (Figure 3 Validation with PD-L1 siRNA Knockdown in HeLa CellsHeLa cells were transfected with control siRNAs (lane 1) orPD-L1 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: MBS154572 (2 ug/mL) and GAPDH (MBS150773, 0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.)
Western Blot (WB) (Figure 4 Validation with PD-L1 over expression in 293 cellsLoading: Lysates/proteins at 15 μg per lane. Lane 1: non-transfected 293 cells Lane 2: PD-L1 overexpressed 293 cells Antibodies: MBS151123 (1 μg/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgGHRP conjugate at 1:10000 dilution.)
Immunohistochemistry (IHC) (Figure 5 Immunohistochemistry Validation of PD-L1 in Human Tonsil CellsImmunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-L1 antibody (MBS151123) at 5ug/ml. Tissue was fixed with formalde hyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
Immunofluorescence (IF) (Figure 6 Immunofluorescence Validation of PD-L1 in Human HeartImmunofluorescent analysis of 4% paraformaldehyde-fixed human heart tissue labeling PD-L1 with MBS151123 at 20μg/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human heart tissue.)
Flow Cytometry (FC) (Figure 7 Flow Cytometry Validation of PD-L1Overlay histogram showing A-20 cells stained with MBS151123 (red line, 1ug/1x106 cells). 1 h incubation at 4°C in 2% FBS/PBS. Followed by secondary antibody 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 1 h 4°C. Isotype control antibody (Green line) was mouse IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Immunohistochemisty (IHC) (Figure 8 Immunohistochemistry Validation of PD-L1 in Rat HeartImmunohistochemical analysis of paraffin-embedded rat heart tissue using anti-PD-L1 antibody (MBS151123) at 5 ug/ml.Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goatanti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin)
Immunohistochemistry (IHC) (Figure 9 Immunohistochemistry Validation of PD-L1 in Human HeartImmunohistochemical analysis of paraffin-embedded human heart tissue using anti-PD-L1 antibody (MBS151123) at 2.5 °g/ml. Tissue was fixed with formalde hyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used assecondary. Counter stained with Hematoxylin.)
Immunofluorescence (IF) (Figure 10 Immunofluorescence Validation of PD-L1 in Rat HeartImmunofluorescence analysis of 4% paraformaldehyde-fixed rat heart tissue labeling PD-L1 with MBS151123 at 20 ug/ml,followed by goat anti-rabbit IgG secondary antibody at 1/250 dilution (red).)
Immunofluorescence (IF) (Figure 11 Immunofluorescence Validation of PD-L1 intumors in Human Cells (Dhar et al., 2018)(A) Several antibody brands were first tested with RBCs,WBCs, and HeLa cells. (MBS151123) was chosen as it provided the highest staining intensity. (B, C) Using the optimal conditions of anti-PDL1 at a concentration of 50ug/mL, following by goat anti-rabbit Alexa Fluor 647, PDL-1 staining was tested on several lung cancer cell lines: (adenocarcinoma), adenocarcinoma), (squamous) and WBCs asa control. (D) Once validated, patient samples were stained for PD-L1, CK, CD45, DAPI.)
Immunofluoresence (Figure 12 Immunohistochemistry Validation of PD-L1in Human Tumors (Gadiot et al., 2011)Immunohistochemical analysis of patient tumors labeling PD-L1 with anti-PD-L1 antibodies (MBS151123). Several anti-PD-L1 antibodies were tested for staining, “Only 1 antibody gave no background staining and was competitively blocked by the addition of PD-L1Fc protein (MBS151123)”.)
Sequence Information (The original sequence contains 12 'U's (yellow highlights) UniProt (reference:: (P49907) that have been mutated to 'S' in the sequence for manufacturing the MBS964014 Bovine Selenoprotein P recombinant protein. The 'U' selenocysteine is encoded by UGA and is typically used as a stop codon in prokaryotic cells, which will lead to early termination of translation. Hence, the lab mutates the selenocysteine which is a derivative of serine. Selenocysteine and cysteine as well as serine are highly similar. The selenocysteine can be regarded as either the 'S' atom of cysteine replaced by Se (selenium atom) , or the 'O' atom of serine replaced by Se (selenium atom). Selenocysteine can mutated to either serine or cysteine. The lab has mutated selenocysteine 'U' to serine for protein expression because mutating 'U' to cysteine can lead to the formation of additional disulfide bonds, which can affect the expression and structure of the recombinant protein)
Western Blot (WB) (Western blot analysis of TMPRSS2 Antibody (N-term) (MBS9215011) in 293, NCI-H460 cell line lysates (35ug/lane). TMPRSS2 (arrow) was detected using the purified Pab.)
Flow Cytometry (FC) (TMPRSS2 Antibody (N-term) (MBS9215011) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies wereused for the analysis.)
Immunohistochemistry (IHC) (TMPRSS2 Antibody (N-term) (RB18784) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TMPRSS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Testing Data (Published customer image:Confocal Microscopy of Mitotic Cells Stained for a-Tubulin and the Nucleolar Protein BN46/51. Five examples of progressively later stages of each mitotic stage are presented. Each image is a Z-axis projection of 0.25 um optical sections through an individual cell. The cell periphery is outlined in white. A. Interphase; B. Prophase; C. Metaphase; D. Anaphase; E. Telophase. F. Selected nuclei of, from left to right, Prophase, Metaphase, Anaphase, and Telophase. Bar = 10 um. Red, BN 46/51; Green, a-tubulin.From: Walsh CJ (2012) The Structure of the Mitotic Spindle and Nucleolus during Mitosis in the Amebo-Flagellate Naegleria. PLoS ONE 7(4): e34763.)
Testing Data (Published customer image:Cap-H2 mutants are defective in anaphase II segregation. Metaphase II and anaphase II morphologies were compared between wild-type and Cap-H2Z3-0019/Cap-H2TH1 mutant males. Testes were stained with DAPI and an anti-tubulin antibody to visualize DNA (white) and microtubules (green), respectively (scale bar in 6A indicates 10 um). (A) Wild-type metaphase/anaphase II cyst. Metaphase II cells are those where each bivalent has congressed to the metaphase plate and appear as a cluster of DAPI staining material. Anaphase II are those cells with two DAPI staining white clusters, indicating homologous chromosome segregation. (B) Metaphase II and anaphase II figures from a Cap-H2 strong mutant. Arrow indicates an anaphase II bridge. The arrowhead highlights an anaphase II bridge or lagging chromosome.From: Hartl TA, Sweeney SJ, Knepler PJ, Bosco G (2008) Condensin II Resolves Chromosomal Associations to Enable Anaphase I Segregation in Drosophila Male Meiosis. PLoS Genet 4(10): e1000228.)
Testing Data (Published customer image:Chromosomes remain associated into anaphase I of Cap-H2 mutants. Metaphase I and anaphase I morphologies were compared between wild-type and Cap-H2Z3-0019/Cap-H2TH1 mutant males. Testes were stained with DAPI and an anti-tubulin antibody to visualize DNA (white) and microtubules (green), respectively (scale bar in 3A indicates 10 um and 5 um in 3G). (A) Metaphase I in the wild-type. Each bivalent has congressed to the metaphase plate and appears as a cluster of DAPI stained material. (B) Anaphase I in the wild-type (DAPI only). Homologous chromosomes have segregated to daughter cells. (C) Anaphase I in the wild-type (DAPI and Tubulin merge). (D) Metaphase I from a Cap-H2Z3-0019/Cap-H2TH1 mutant male appears wild-type. (E) Anaphase I from a Cap-H2Z3-0019/Cap-H2TH1 mutant male (DAPI only). Chromatin bridges can be seen in three different segregation events. (F) Anaphase I from a Cap-H2Z3-0019/Cap-H2TH1 mutant male (DAPI and Tubulin merge). (G) Higher resolution wild-type anaphase I image showing complete segregation of homologs. (H) Anaphase I from a Cap-H2Z3-0019/Cap-H2TH1 mutant demonstrating extensive chromatin bridging due to persistent associations between chromosomes migrating to opposing poles. (I) Anaphase I bridge found from a Cap-D3EY00456 mutant.From: Hartl TA, Sweeney SJ, Knepler PJ, Bosco G (2008) Condensin II Resolves Chromosomal Associations to Enable Anaphase I Segregation in Drosophila Male Meiosis. PLoS Genet 4(10): e1000228.)
Testing Data (Published customer image: Role of the phosphorylation of ATF7 in G2/M progression. (A) Parental HeLa S3/TR, HeLa S3/TR/ATF7-wt (cl.2), or HeLa S3/TR/ATF7-TA (cl.1) cells were synchronized using DTB and released into thymidine-free medium containing 1 ug/ml Dox for 12?h. Whole cell lysates were analyzed by WB. Full-length blots are presented in S12A Fig. (B) HeLa S3/TR/ATF7-wt (cl.2) (upper panels) or HeLa S3/TR/ATF7-TA (cl.1) cells (lower panels) were synchronized using single-thymidine block and released into thymidine-free medium containing 1 ug/ml Dox for 11 h. Cells were triply stained with anti-ATF7[SAB2500131] and anti-a-tubulin antibodies and PI (for DNA). Scale bars, 20 um. (C, D) Cells were stained with anti-histone H3pS10 antibody (for M phase) and PI for analyzing cell-cycle progression by flow cytometry. (C) Parental HeLa S3/TR, HeLa S3/TR/ATF7-wt (cl.2), or HeLa S3/TR/ATF7-TA (cl.1) cells were synchronized using DTB and released into thymidine-free medium containing 1 ug/ml Dox for 10.5~12.5 h. Two-dimensional histograms (DNA vs histone H3pS10) are presented together with DNA histograms, and the percentages of cells in G1 and M phases were measured. Cells in M and and G1 phases were quantitated from the results of S4 Fig. Values are means +/- SD (three independent clones). Asterisks indicate the significant difference (*P)
Testing Data (Published customer image:T566 phosphorylation affects Bub1 stability. (A) BUB1-T566A mutant cells do not show substantial delay in recovering from nocodazole arrest. Wild-type (WT) and BUB1-T566A mutant cells were incubated with nocodazole (15 ug/mL) at 30 degree C for 90 min and then released from nocodazole arrest; at the indicated times, samples were taken to measure rebudded cells. (B) BUB1-T566A mutant cells show no significant sensitivity to nocodazole in a survival assay. Wild-type (WT), bub1?, and BUB1-T566A cells were incubated with nocodazole (15 ug/mL); at the indicated times, cells were washed out and approximately 200 cells were plated on a YPD plate. Cell viability was calculated by dividing the number of colonies formed at the 2.5 and 5 h time points by that formed in the absence of nocodazole (0 h) at 30 degree C. (C) Phosphorylation of T566 is important for degradation of Bub1 during anaphase but not during G1. cdc15-2 cells with HA-tagged Bub1 or Bub1-T566A expressed by the GAL1 promoter (GAL-BUB1 and GAL-BUB1-T566A) were arrested in anaphase (cdc15-2) or in G1 (alpha-factor) and then transferred to medium containing galactose for 2 h to induce Bub1 expression. Glucose was added to shut-off Bub1 expression; samples were taken at the indicated times for Western blot analyses with antibody to the HA epitope. Tubulin was used as a loading control. (D) The Bub1-T566A protein is more stable than wild-type in the presence of nocodazole. Wild-type and BUB1-T566A mutant cells (Bub1 and Bub1-T566A are tagged with myc) were incubated in the presence of nocodazole (10 ug/mL) at 30 degree C; at the indicated times, samples were taken for Western blot analyses with antibody to the myc epitope. Tubulin was used as a loading control.From: Goto GH, Mishra A, Abdulle R, Slaughter CA, Kitagawa K (2011) Bub1-Mediated Adaptation of the Spindle Checkpoint. PLoS Genet 7(1): e1001282.)
Testing Data (Published customer image:Analysis of the Co-localization of Tubulin and the Nucleolar Protein BN46/51 During Prophase. Single 0.25 um optical sections at 1.0 um intervals are presented for two different cells. Columns A and D; raw data. Columns B and E; tubulin pixels are only displayed if the corresponding nucleolar protein pixel equaled or exceeded 25% of the maximum possible intensity. Columns C and F; tubulin pixels are only displayed if the corresponding nucleolar protein pixel equaled or exceeded 50% of maximum possible intensity. Numerical values are the summed fraction of tubulin intensity remaining in a given section after the subtraction. Red, BN 46/51; Green, a-tubulin. Bar = 10 um.From: Walsh CJ (2012) The Structure of the Mitotic Spindle and Nucleolus during Mitosis in the Amebo-Flagellate Naegleria. PLoS ONE 7(4): e34763.)
Testing Data (Published customer image:Stages of Mitosis in Naegleria Revealed by Staining for a-Tubulin, BN46/51 and DAPI. Images were obtained by conventional epifluorescence microscopy. A. Interphase; B. Prophase; C. Metaphase; D. Anaphase; E. Telophase. Bar = 10 um. Red, BN46/51; Green, a-tubulin; Blue, DAPI.Walsh CJ (2012) The Structure of the Mitotic Spindle and Nucleolus during Mitosis in the Amebo-Flagellate Naegleria. PLoS ONE 7(4): e34763.)
Testing Data (HeLa cells stained with Rat anti tubulin apha green, nuclei are counterstained with DAPI)
Testing Data (Western blot analysis of CD107b on J774 cell lysate probed with Rat anti Mouse CD107b (MBS212662) at 1/500, 1/1000, 1/2000. Rat anti tubulin alpha is included as a loading control. Detection is with Goat anti Rat IgG:Dylight® (MBS224270))
Western Blot (WB) (Western blot analysis of extracts from TSA treated HeLa cells, using Acetyl-FKHR(K294) Ab.Lane 1: Heat-shock treated COS-7, blocked with antigen-specific peptidesLane 2: Heat-shock treated COS-7Lane 3: LPS treated MBS127101 cells. Observed bands: 90 kDa)
Immunohistochemistry (IHC) (MBS9600633 at 1/100 staining Human lung cancer by IHC-P. The
sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF alpha Monoclonal Antibody (P/T2).)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human pancreas stained with TNF alpha Mouse Monoclonal Antibody (P/T2).)
Immunofluorescence (IF) (Immunofluorescence staining of HePG2 cells using TNF alpha Mouse Monoclonal Antibody (P/T2) followed by goat anti-mouse IgG-CF488. Counterstained with RedDot.)
Western Blot (WB) (The anti-ACE2 C-term Pab (MBS9212149) is used in Western blot to detect ACE2 in 293 cell lysate.)
Western Blot (WB) (Western blot analysis of anti-ACE2 C-term Pab (MBS9212149)in K562 cell line lysates (35ug/lane). ACE2(arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human testis tissue reacted with ACE2 (SARS Receptor) Antibody (C-term) (MBS9212149), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB) (Western blot analysis on JK using alpha-SMA antibody, The lane on the left is blocked with the antigen-specific peptide.)
Immunohistochemistry (IHC) (MBS9600621 at 1/100 staining mouse pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) (MBS9600621 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) (MBS9600621 at 1/100 staining mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) (MBS9600621 at 1/100 staining human skin tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) (MBS9600621 at 1/100 staining human appendiceal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) (MBS9600621 at 1/100 staining human seminoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) (MBS9600621 at 1/100 staining rat uterine tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) (MBS9600621 at 1/100 staining rat gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) (MBS9600621 at 1/100 staining rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (IHC) ( The expression of alpha-SMA and alpha-Ac-Tub in lung tissue measured by immunohistochemistry)
Immunofluorescence (IF) (AF1032 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF1032) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)
Immunohistochemistry (IHC) (AF1032 at 1/100 staining Rat colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of E Cadherin expression in HEK293T (A), mouse lung (B), mouse kidney (C), rat lung (D), rat kidney (E) whole cell lysates. )
Immunohistochemistry (IHC) (Immunohistochemical analysis of E Cadherin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF) (Immunofluorescent analysis of E Cadherin staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunoprecipitation (IP) (Immunoprecipitation of E Cadherin from 0.5mg HEK293F whole cell extract lysate, using 5ug of Anti-E Cadherin Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, HEK293F whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with Anti-E Cadherin Antibody. )
Western Blot (WB) (Western blot analysis of extracts from MCF7 cells using Estrogen Receptor-a(Phospho-Ser167) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Phospho-Ser167) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-a(Phospho-Ser167) Antibody.)
Western Blot (WB) (Western blot analysis of extracts from HepG2, using ENaC yAntibody.)
Immunohistochemistry (IHC) (MBS9608857 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody)
Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using TGF beta Antibody. The lane on the left is treated with the synthesized peptide.)
Immunohistochemistry (IHC) (MBS9600620 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600620 at 1/100 staining mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600620 at 1/100 staining mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600620 at 1/100 staining human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600620 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600620 at 1/100 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600620 at 1/100 staining rat tumor tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600620 at 1/100 staining rat gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9600620 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (TGF beta1 Antibody for IHC in human kidney tissue.)
Immunohistochemistry (IHC) (MBS9600620 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunofluorescence (IF) (MBS9600620 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9600620 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)