RPMI 1640 Medium w/o L-Alanine, L-Glutamine, Folic acid, Riboflavin Culture Media
RPMI 1640 Medium w/o L-Alanine, L-Glutamine, Folic acid, Riboflavin (Powder)
Purity
Cell Culture Grade
Synonyms
RPMI 1640 Medium w/o L-Alanine; L-Glutamine; Folic acid; Riboflavin; Riboflavin (Powder); Riboflavin culture media
Purity/Purification
Cell Culture Grade
Form/Format
Light pink, homogenous, free flowing powder
Endotoxin Level
<=1EU/ml
pH
8.6
Solubility
Pink. clear, complete
Directions per Liter
Dissolve 10.092g in 900ml of ddH2O, stirring gently until completely solubilized. Do NOT heat. If required, add 2.0g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22 micron filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.
Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.
Media Formulation
Components shown as g/L
Inorganic Salts
Calcium Nitrate*4H2O: 0.100
Magnesium Sulfate: 0.04884
Potassium Chloride: 0.400
Sodium Chloride: 6.000
Sodium Phosphate Dibasic: 0.800
Amino: Acids
L-Alanine: Absent
L-Arginine: 0.200
L-Asparagine: 0.050
L-Aspartic Acid: 0.020
L-Cystine*2HCl: 0.065
L-Glutamic Acid: 0.020
L-Glutamine: Absent
Glycine: 0.010
L-Histidine: 0.015
Hydroxy-L-proline: 0.020
L-Isoleucine: 0.050
L-Leucine: 0.050
L-Lysine*HCl: 0.040
L-Methionine: 0.015
L-Phenylalanine: 0.015
L-Proline: 0.020
L-Serine: 0.030
L-Threonine: 0.020
L-Tryptophan: 0.005
L-Tyrosine*HCl: 0.028
L-Valine: 0.020
Vitamins
p-Aminobenzoic Acid: 0.001
D-Biotin: 0.0002
Choline Chloride: 0.003
Cyanocobalamin (Vit.B12): 0.000005
Folic Acid: Absent
myo-Inositol: 0.035
Niacinamide: 0.001
D-Pantothenic Acid, Ca: 0.00025
Pyridoxal*HCl: 0.001
Riboflavin: Absent
Thiamine*HCl: 0.001
Other
D-Glucose: 2.000
Glutathione, reduced: 0.001
Phenol Red, Sodium: 0.0053
Total: 10.092g/L
Inorganic Salts
Calcium Nitrate*4H2O: 0.100
Magnesium Sulfate: 0.04884
Potassium Chloride: 0.400
Sodium Chloride: 6.000
Sodium Phosphate Dibasic: 0.800
Amino: Acids
L-Alanine: Absent
L-Arginine: 0.200
L-Asparagine: 0.050
L-Aspartic Acid: 0.020
L-Cystine*2HCl: 0.065
L-Glutamic Acid: 0.020
L-Glutamine: Absent
Glycine: 0.010
L-Histidine: 0.015
Hydroxy-L-proline: 0.020
L-Isoleucine: 0.050
L-Leucine: 0.050
L-Lysine*HCl: 0.040
L-Methionine: 0.015
L-Phenylalanine: 0.015
L-Proline: 0.020
L-Serine: 0.030
L-Threonine: 0.020
L-Tryptophan: 0.005
L-Tyrosine*HCl: 0.028
L-Valine: 0.020
Vitamins
p-Aminobenzoic Acid: 0.001
D-Biotin: 0.0002
Choline Chloride: 0.003
Cyanocobalamin (Vit.B12): 0.000005
Folic Acid: Absent
myo-Inositol: 0.035
Niacinamide: 0.001
D-Pantothenic Acid, Ca: 0.00025
Pyridoxal*HCl: 0.001
Riboflavin: Absent
Thiamine*HCl: 0.001
Other
D-Glucose: 2.000
Glutathione, reduced: 0.001
Phenol Red, Sodium: 0.0053
Total: 10.092g/L
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Related Product Information for RPMI 1640 Medium w/o L-Alanine, L-Glutamine, Folic acid, Riboflavin culture media
Description: RPMI-1640 was developed by Moore, et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.
Product Categories/Family for RPMI 1640 Medium w/o L-Alanine, L-Glutamine, Folic acid, Riboflavin culture media
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Product Notes
The RPMI 1640 Medium w/o L-Alanine, L-Glutamine, Folic acid, Riboflavin (Catalog #AAA653004) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RPMI 1640 Medium w/o L-Alanine, L-Glutamine, Folic acid, Riboflavin, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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