Rabbit Major Basic Protein (MBP) Polyclonal Antibody | anti-MBP antibody
Polyclonal Antibody to Major Basic Protein (MBP)
Gene Names
Prg2; MBP; EMBP; mMBP; mMBP-1
Reactivity
Mus musculus (Mouse)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Major Basic Protein (MBP); Polyclonal Antibody; Polyclonal Antibody to Major Basic Protein (MBP); anti-MBP antibody
Host
Rabbit
Reactivity
Mus musculus (Mouse)
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol
Concentration
0.52mg/ml (varies by lot)
Applicable Applications for anti-MBP antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Rat
Immunogen
Recombinant MBP (Arg37~Lys215) expressed in E.coli (MBS2029772).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot;Sample: Recombinant MBP, Mouse.)
Western Blot (WB)
(Western Blot:Sample:Lane 1: Rat Liver Tissue;Lane 2: Mouse Brain Tissue; Lane 3: Rat Brain Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P.Samples: Mouse Tissue)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
bone marrow proteoglycan
NCBI Official Synonym Full Names
proteoglycan 2, bone marrow
NCBI Official Symbol
Prg2
NCBI Official Synonym Symbols
MBP; EMBP; mMBP; mMBP-1
NCBI Protein Information
bone marrow proteoglycan
UniProt Protein Name
Bone marrow proteoglycan
Protein Family
UniProt Gene Name
Prg2
UniProt Synonym Gene Names
Mbp-1; BMPG; EMBP; MBP
Uniprot Description
PRG2: Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.
Protein type: Secreted; Secreted, signal peptide; Vesicle
Chromosomal Location of Human Ortholog: 2 D|2 49.45 cM
Molecular Function: carbohydrate binding
Biological Process: defense response to bacterium; defense response to nematode; immune response; immune system process; negative regulation of interleukin-10 production; positive regulation of interleukin-4 production; regulation of cytokine biosynthetic process
