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Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
1205 results for "Rat IgG Isotype Control" - showing 1000-1050
Flow Cytometry (FC/FACS)
(Con A-stimulated (3 days) C57/BL6 splenocytes were stained with anti-CD25 (clone: PC61.5.3)(filled histogram) or rat IgG1 isotype control (open histogram).)
CD44, Monoclonal Antibody (Cat# AAA524554)
Full Name
Anti-Human CD44, Biotin, (clone: BU75)(Mouse IgG2a)
Reactivity
Mouse
Applications
FC/FACS, IHC, IF, WB, FA
Pricing
$305/0.1 mg | $1,160/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of human peripheral blood monocytes using anti-CD40L antibody (MBS488580) AT161-10 Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of AT161-10 (MBS488580) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488580, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
CD40L, Monoclonal Antibody (Cat# AAA488579)
Full Name
Anti-CD40L [AT161-10]
Reactivity
Human
Applications
EIA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of RAW 264.7 cells using anti-Transferrin receptor antibody (MBS488646) R17 217.1.3. Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of R17 217.1.3 (MBS488646) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488646, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Transferrin receptor, Monoclonal Antibody (Cat# AAA488647)
Full Name
Anti-Transferrin receptor [R17 217.1.3]
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Tspan1 using anti-Tspan1 antibody (MBS1753785).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat small intestine tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Tspan1 antigen affinity purified polyclonal antibody (Catalog # MBS1753785) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Tspan1 at approximately 28-30KD. The expected band size for Tspan1 is at 28-30KD. )
Tspan1, Polyclonal Antibody (Cat# AAA1753785)
Full Name
Anti-Tspan1 Antibody
Reactivity
Rat
Applications
WB, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Flow Cytometry (FC/FACS)
(Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-THRB1 antibody (bs-11440R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20 degree C. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. )
THRB1, Polyclonal Antibody (Cat# AAA9234076)
Full Name
THRB1 Polyclonal Antibody
Reactivity
Rat, Cow
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Western Blot (WB)
(Western Blot using anti-CD266 antibody 10E5 (MBS488668) Mouse spleen tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 10E5 () at 2ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
CD226, Monoclonal Antibody (Cat# AAA488668)
Full Name
Anti-CD226 [10E5]
Gene Names
Cd226; Pta1; DNAM1; DNAM-1; TLiSA1; BC051526
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of RNase L/Rnasel using anti-RNase L/Rnasel antibody (MBS1753534).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: mouse lung tissue lysatesLane 2: mouse Raw264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-RNase L/Rnasel antigen affinity purified polyclonal antibody (Catalog # MBS1753534) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for RNase L/Rnasel at approximately 83KD. The expected band size for RNase L/Rnasel is at 83KD. )
RNase L/Rnasel, Polyclonal Antibody (Cat# AAA1753534)
Full Name
Anti-RNase L/Rnasel Antibody
Gene Names
Rnasel; E230029I04Rik
Reactivity
Mouse, Rat
Applications
WB, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody , green in A and Rat anti Mouse CD8 antibody , red in B, Merged image in C)
CD11b, Monoclonal Antibody (Cat# AAA226102)
Full Name
Rat anti Mouse CD11b:Amethyst Orange
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$500/0.1 mg | $2,090/5x0.1 mg
Immunohistochemistry (IHC)-Paraffin
(Tissue/cell: Mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min; Incubation: Anti-Caspase-1 p20 Polyclonal Antibody, Unconjugated(bs-10442R) 1:500, overnight at 4?C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining )
Caspase-1 p20, Polyclonal Antibody (Cat# AAA9233839)
Full Name
Caspase-1 p20 Polyclonal Antibody
Reactivity
Rat
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Immunohistochemistry (IHC)-Paraffin
(Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min; Incubation: Anti-MCKD2/UMOD Polyclonal Antibody, Unconjugated(bs-2189R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining )
Uromucoid, Polyclonal Antibody (Cat# AAA9237549)
Full Name
Uromucoid Polyclonal Antibody
Gene Names
Umod; THP; Urehd1; urehr4
Reactivity
Rat, Cow
Applications
WB, IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Western Blot (WB)
(Figure 1. Western blot analysis of SHMT1 using anti-SHMT1 antibody (MBS1754016).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: monkey liver tissue lysatesLane 2: rat liver tissue lysatesLane 3: mouse liver tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- SHMT1 antigen affinity purified monoclonal antibody (Catalog # MBS1754016) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for SHMT1 at approximately 53KD. The expected band size for SHMT1 is at 53KD. )
SHMT1, Monoclonal Antibody (Cat# AAA1754016)
Full Name
Anti-SHMT1 Antibody (monoclonal, 9C7)
Gene Names
SHMT1; SHMT; CSHMT
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of NOXA2/p67phox/NCF2 using anti-NOXA2/p67phox/NCF2 antibody (MBS1753525).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human THP-1 whole cell lysatesLane 2: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-NOXA2/p67phox/NCF2 antigen affinity purified polyclonal antibody (Catalog # MBS1753525) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for NOXA2/p67phox/NCF2 at approximately 60KD. The expected band size for NOXA2/p67phox/NCF2 is at 60KD. )
NOXA2/p67phox/NCF2, Polyclonal Antibody (Cat# AAA1753525)
Full Name
Anti-NOXA2/p67phox/NCF2 Antibody
Gene Names
NCF2; NCF-2; NOXA2; P67PHOX; P67-PHOX
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescence (IF)
(Figure 1. IF analysis of PAR4/Pawr using anti-PAR4/Pawr antibody (MBS1753603).PAR4/Pawr was detected in immunocytochemical section of MFC cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- PAR4/Pawr Antibody (MBS1753603) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
PAR4/Pawr, Polyclonal Antibody (Cat# AAA1753603)
Full Name
Anti-PAR4/Pawr Antibody
Gene Names
Pawr; PAR4; Par-4; 2310001G03Rik
Reactivity
Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Glycine decarboxylase/GLDC using anti-Glycine decarboxylase/GLDC antibody (MBS1753673).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEPG2 whole cell lysatesLane 2: human placenta tissue lysatesLane 3: human HEK293 whole cell lysatesLane 4: human T47D whole cell lysatesLane 5: human A549 whole cell lysatesLane 6: rat liver tissue lysatesLane 7: rat kidney tissue lysatesLane 8: mouse liver tissue lysatesLane 9: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Glycine decarboxylase/GLDC antigen affinity purified polyclonal antibody (Catalog # MBS1753673) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Glycine decarboxylase/GLDC at approximately 113KD. The expected band size for Glycine decarboxylase/GLDC is at 113KD. )
Glycine decarboxylase/GLDC, Polyclonal Antibody (Cat# AAA1753673)
Full Name
Anti-Glycine decarboxylase/GLDC Antibody
Gene Names
GLDC; GCE; GCSP; HYGN1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Synaptopodin/SYNPO using anti- Synaptopodin/SYNPO antibody (MBS1753575).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysatesLane 3: human SH-SY5Y whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Synaptopodin/SYNPO antigen affinity purified polyclonal antibody (Catalog # MBS1753575) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Synaptopodin/SYNPO at approximately 99KD. The expected band size for Synaptopodin/SYNPO is at 99KD. )
Synaptopodin/SYNPO, Polyclonal Antibody (Cat# AAA1753575)
Full Name
Anti-Synaptopodin/SYNPO Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescence (IF)
(Figure 2. IF analysis of Golm1/GOLPH2 using anti- Golm1/GOLPH2 antibody (MBS1753566).Golm1/GOLPH2 was detected in immunocytochemical section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- Golm1/GOLPH2 Antibody (MBS1753566) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Golm1/GOLPH2, Polyclonal Antibody (Cat# AAA1753566)
Full Name
Anti-Golm1/GOLPH2 Antibody
Gene Names
Golm1; GP73; Golph2; AW125446; PSEC0257; 2310001L02Rik; D030064E01Rik
Reactivity
Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, ICC, IF, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of DDX1 using anti-DDX1 antibody (MBS1754028).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human CACO-2 whole cell lysatesLane 3: human U20S whole cell lysatesLane 4: human HEK293 whole cell lysatesLane 5: human U87 whole cell lysatesLane 6: human HELA whole cell lysatesLane 7: human A549 whole cell lysatesLane 8: rat kidney tissue lysatesLane 9: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- DDX1 antigen affinity purified monoclonal antibody (Catalog # MBS1754028) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for DDX1 at approximately 86KD. The expected band size for DDX1 is at 86KD. )
DDX1, Monoclonal Antibody (Cat# AAA1754028)
Full Name
Anti-DDX1 Antibody (monoclonal, 2D4)
Gene Names
DDX1; DBP-RB; UKVH5d
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of IRF3 using anti-IRF3 antibody (MBS1753962).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse lung tissue lysatesLane 3: mouse brain tissue lysatesLane 4: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- IRF3 antigen affinity purified monoclonal antibody (Catalog # MBS1753962) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for IRF3 at approximately 50-55KD. The expected band size for IRF3 is at 50-55KD. )
IRF3, Monoclonal Antibody (Cat# AAA1753962)
Full Name
Anti-IRF3 Antibody (monoclonal, 7B10)
Gene Names
Irf3; IRF-3; C920001K05Rik
Reactivity
Mouse, Rat
Applications
WB, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of IRF3 using anti-IRF3 antibody (MBS1753960).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat lung tissue lysatesLane 2: rat brain tissue lysatesLane 3: rat C6 whole cell lysatesLane 4: rat RH-35 whole cell lysatesLane 5: mouse lung tissue lysatesLane 6: mouse brain tissue lysatesLane 7: mouse NIH/3T3 whole cell lysatesLane 8: mouse SP2/0 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- IRF3 antigen affinity purified monoclonal antibody (Catalog # MBS1753960) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for IRF3 at approximately 50-55KD. The expected band size for IRF3 is at 50-55KD. )
IRF3, Monoclonal Antibody (Cat# AAA1753960)
Full Name
Anti-IRF3 Antibody (monoclonal, 11H2)
Gene Names
Irf3; IRF-3; C920001K05Rik
Reactivity
Mouse, Rat
Applications
WB, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Profilin 2/PFN2 using anti-Profilin 2/PFN2 antibody (MBS1753737).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human U87 whole cell lysatesLane 2: human PC-3 whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: human Hela whole cell lysatesLane 5: human HEK293 whole cell lysatesLane 6: rat brain tissue lysatesLane 7: mouse brain tissue lysatesLane 8: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Profilin 2/PFN2 antigen affinity purified polyclonal antibody (Catalog # MBS1753737) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Profilin 2/PFN2 at approximately 15KD. The expected band size for Profilin 2/PFN2 is at 15KD. )
Profilin 2/PFN2, Polyclonal Antibody (Cat# AAA1753737)
Full Name
Anti-Profilin 2/PFN2 Antibody
Gene Names
PFN2; PFL; D3S1319E
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PDIA6 using anti-PDIA6 antibody (MBS1753620).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human HepG2 whole cell lysatesLane 3: human HT1080 whole cell lysatesLane 4: human Raji whole cell lysatesLane 5: human Hela whole cell lysatesLane 6: human HEK293 whole cell lysatesLane 7: human THP-1 whole cell lysatesLane 8: human SH-SY5Y whole cell lysatesLane 9: rat kidney tissue lysatesLane 10: rat heart tissue lysatesLane 11: rat liver tissue lysatesLane 12: rat lung tissue lysatesLane 13: mouse kidney tissue lysatesLane 14: mouse heart tissue lysatesLane 15: mouse liver tissue lysatesLane 16: mouse lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PDIA6 antigen affinity purified polyclonal antibody (Catalog # MBS1753620) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PDIA6 at approximately 50KD. The expected band size for PDIA6 is at 50KD. )
PDIA6, Polyclonal Antibody (Cat# AAA1753620)
Full Name
Anti-PDIA6 Antibody
Gene Names
PDIA6; P5; ERP5; TXNDC7
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Transketolase/TKT using anti-Transketolase/TKT antibody (MBS1754007).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human SW620 whole cell lysatesLane 4: human Raji whole cell lysatesLane 5: rat liver tissue lysatesLane 6: rat RH35 whole cell lysatesLane 7: mouse liver tissue lysatesLane 8: mouse HEPA1-6 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Transketolase/TKT antigen affinity purified monoclonal antibody (Catalog # MBS1754007) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for Transketolase/TKT at approximately 68KD. The expected band size for Transketolase/TKT is at 68KD. )
Transketolase/TKT, Monoclonal Antibody (Cat# AAA1754007)
Full Name
Anti-Transketolase/TKT Antibody (monoclonal, 3E5)
Gene Names
TKT; TK; TKT1; HEL107
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of XPC using anti-XPC antibody (MBS1753329).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human MCF-7whole cell lysatesLane 2: human A549 whole cell lysatesLane 3: rat liver tissue lysatesLane 4: mouse lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-XPC antigen affinity purified polyclonal antibody (Catalog # MBS1753329) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for XPC at approximately 106KD. The expected band size for XPC is at 106KD. )
XPC, Polyclonal Antibody (Cat# AAA1753329)
Full Name
Anti-XPC Antibody
Gene Names
XPC; XP3; RAD4; XPCC; p125
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of SBCAD/ACADSB using anti-SBCAD/ACADSB antibody (MBS1753792).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A431 whole cell lysatesLane 2: human T47D whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: monkey Cos-7 whole cell lysatesLane 5: rat liver tissue lysatesLane 6: mouse liver tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SBCAD/ACADSB antigen affinity purified polyclonal antibody (Catalog # MBS1753792) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SBCAD/ACADSB at approximately 40-44KD. The expected band size for SBCAD/ACADSB is at 47KD. )
SBCAD/ACADSB, Polyclonal Antibody (Cat# AAA1753792)
Full Name
Anti-SBCAD/ACADSB Antibody
Gene Names
ACADSB; ACAD7; SBCAD; 2-MEBCAD
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of hnRNP D/AUF1/HNRNPD using anti-hnRNP D/AUF1/HNRNPD antibody (MBS1754046).hnRNP D/AUF1/HNRNPD was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-hnRNP D/AUF1/HNRNPD Antibody (MBS1754046) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
hnRNP D/AUF1/HNRNPD, Monoclonal Antibody (Cat# AAA1754046)
Full Name
Anti-hnRNP D/AUF1/HNRNPD Antibody (monoclonal, 2B12)
Gene Names
HNRNPD; P37; AUF1; AUF1A; HNRPD; hnRNPD0
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Calpain 1 using anti-Calpain 1 antibody (MBS1754003).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: human PC-3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Calpain 1 antigen affinity purified monoclonal antibody (Catalog # MBS1754003) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for Calpain 1 at approximately 82KD. The expected band size for Calpain 1 is at 82KD. )
Calpain 1, Monoclonal Antibody (Cat# AAA1754003)
Full Name
Anti-Calpain 1 Antibody (monoclonal, 2E3)
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human
Applications
WB, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CCRL2 antibody BZ2E3 (MBS488612) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of BZ2E3 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
CCRL2, Monoclonal Antibody (Cat# AAA488612)
Full Name
Anti-CCRL2 [BZ2E3]
Gene Names
Ccrl2; E01; Ackr5; CCR11; L-CCR; Cmkbr1l2; 1810047I05Rik
Reactivity
Mouse
Applications
FC/FACS, WB, EIA, IP, IHC, ICC, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of DCTN1/p150-glued using anti-DCTN1/p150-glued antibody (MBS1753498).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human placenta tissue lysatesLane 4: human U-87MG whole cell lysatesLane 5: human Caco-2 whole cell lysatesLane 6: human U20S whole cell lysatesLane 7: human HEK293 whole cell lysatesLane 8: human HepG2 whole cell lysatesLane 9: rat brain tissue lysatesLane 10: rat stomach tissue lysatesLane 11: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-DCTN1/p150-glued antigen affinity purified polyclonal antibody (Catalog # MBS1753498) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for DCTN1/p150-glued at approximately 150KD. The expected band size for DCTN1/p150-glued is at 150KD. )
DCTN1/p150-glued, Polyclonal Antibody (Cat# AAA1753498)
Full Name
Anti-DCTN1/p150-glued Antibody
Gene Names
DCTN1; P135; DP-150; DAP-150
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Aconitase 1/ACO1 using anti-Aconitase 1/ACO1 antibody (MBS1753551).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human HepG2 whole cell lysatesLane 3: human U20S wholel cell lysatesLane 4: monkey liver tissue lysatesLane 5: rat liver tissue lysatesLane 6: rat kidney tissue lysatesLane 7: mouse liver tissue lysatesLane 8: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Aconitase 1/ACO1 antigen affinity purified polyclonal antibody (Catalog # MBS1753551) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Aconitase 1/ACO1 at approximately 98KD. The expected band size for Aconitase 1/ACO1 is at 98KD. )
Aconitase 1/ACO1, Polyclonal Antibody (Cat# AAA1753551)
Full Name
Anti-Aconitase 1/ACO1 Antibody
Gene Names
ACO1; IRP1; ACONS; IREB1; IREBP; IREBP1
Reactivity
Human, Mouse, Monkey, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of HOXD11 using anti-HOXD11 antibody (MBS1753818).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human Hek293 whole cell lysatesLane 3: human U87 whole cell lysatesLane 4: human A431 whole cell lysatesLane 5: human A549 whole cell lysatesLane 6: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-HOXD11 antigen affinity purified polyclonal antibody (Catalog # MBS1753818) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for HOXD11 at approximately 45KD. The expected band size for HOXD11 is at 45KD. )
HOXD11, Polyclonal Antibody (Cat# AAA1753818)
Full Name
Anti-HOXD11 Antibody
Gene Names
HOXD11; HOX4; HOX4F
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western Blot using anti-CCRL2 antibody (MBS488610) Mouse thymus (A) and mouse brain (B) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of CCRL2 (MBS488610) at 0.5ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
CCRL2, Monoclonal Antibody (Cat# AAA488611)
Full Name
Anti-CCRL2 [BZ5B8]
Gene Names
Ccrl2; E01; Ackr5; CCR11; L-CCR; Cmkbr1l2; 1810047I05Rik
Reactivity
Mouse
Applications
FC/FACS, WB, EIA, IP, IHC, ICC, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of UBE2D1/2/3/4 using anti-UBE2D1/2/3/4 antibody (MBS1753670).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat heart tissue lysatesLane 2: rat lung tissue lysatesLane 3: rat liver tissue lysatesLane 4: human HEK293 whole cell lysatesLane 5: human Jurkat whole cell lysatesLane 6: human CACO-2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-UBE2D1/2/3/4 antigen affinity purified polyclonal antibody (Catalog # MBS1753670) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for UBE2D1/2/3/4 at approximately 17KD. The expected band size for UBE2D1/2/3/4 is at 17KD. )
UBE2D1/2/3/4, Polyclonal Antibody (Cat# AAA1753670)
Full Name
Anti-UBE2D1/2/3/4 Antibody
Gene Names
UBE2D1; SFT; UBCH5; UBC4/5; UBCH5A; E2(17)KB1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of CISD2 using anti-CISD2 antibody (MBS1753731).CISD2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CISD2 Antibody (MBS1753731) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
CISD2, Polyclonal Antibody (Cat# AAA1753731)
Full Name
Anti-CISD2 Antibody
Gene Names
CISD2; ERIS; WFS2; ZCD2; NAF-1; Miner1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of EGFR using anti-EGFR antibody (MBS1753263).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human U87 whole cell lysatesLane 3: human A431 whole cell lysatesLane 4: human A549 whole cell lysatesLane 5: human Hela whole cell lysatesLane 6: rat liver tissue lysatesLane 7: mouse liver tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-EGFR antigen affinity purified polyclonal antibody (Catalog # MBS1753263) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for EGFR at approximately 180KD. The expected band size for EGFR is at 180KD. )
EGFR, Polyclonal Antibody (Cat# AAA1753263)
Full Name
Anti-EGFR Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD40 antibody 3/23 (MBS488634) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 3/23 (MBS488634) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488634, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
CD40, Monoclonal Antibody (Cat# AAA488635)
Full Name
Anti-CD40 [3/23]
Gene Names
Cd40; IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
Reactivity
Mouse
Applications
FC/FACS, IHC, IP
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Calpain 1 using anti-Calpain 1 antibody (MBS1754002).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: human PC-3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Calpain 1 antigen affinity purified monoclonal antibody (Catalog # MBS1754002) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for Calpain 1 at approximately 82KD. The expected band size for Calpain 1 is at 82KD. )
Calpain 1, Monoclonal Antibody (Cat# AAA1754002)
Full Name
Anti-Calpain 1 Antibody (monoclonal, 2I10)
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human
Applications
WB, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Claudin 3/CLDN3 using anti-Claudin 3/CLDN3 antibody (MBS1753659).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysatesLane 2: human Caco-2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Claudin 3/CLDN3 antigen affinity purified polyclonal antibody (Catalog # MBS1753659) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Claudin 3/CLDN3 at approximately 23KD. The expected band size for Claudin 3/CLDN3 is at 23KD. )
Claudin 3/CLDN3, Polyclonal Antibody (Cat# AAA1753659)
Full Name
Anti-Claudin 3/CLDN3 Antibody
Gene Names
CLDN3; RVP1; HRVP1; C7orf1; CPE-R2; CPETR2
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
IgG, Polyclonal Isotype Control (Cat# AAA1564650)
Full Name
Rat IgG Isotype Control Antibody
Applications
FC/FACS/FCM
Purity
>95%; Protein A or G purified.
Flow Cytometry (FC/FACS)
(Blank control (blue line): U251 (fixed with 70% ethanol for 5 min at -20 degree C). Primary Antibody (green line): Rabbit Anti- Neuroligin 2 antibody (bs-11098R), Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1 µg /test. )
NLGN2, Polyclonal Antibody (Cat# AAA9233945)
Full Name
NLGN2 Polyclonal Antibody
Reactivity
Rat, Pig, Dog, Cow
Applications
IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Balb/c peritoneal macrophages (left) or bone marrow (Gr-1 gate)(right) were stained with anti-C5a receptor (CD88)(clone: 20/70)(filled histogram) or rat IgG2b isotype control (open histogram).)
CD8a, Monoclonal Antibody (Cat# AAA524523)
Full Name
Anti-Mouse CD8a (Ly 2), APC (Clone CT-CD8a)(rat IgG2a)
Reactivity
Mouse
Applications
FC/FACS, IHC-F, IHC-P
Pricing
$565/0.1 mg | $2,320/5x0.1 mg
Flow Cytometry (FC/FACS)
(C57BL/6 mouse splenic T-cells (left) or thymocytes (right) were stained with anti-CD8a (clone: CT-CD8a)(filled histogram) or rat IgG2a isotype control (open histogram).)
C5a Receptor, Monoclonal Antibody (Cat# AAA524538)
Full Name
Anti-Mouse C5a Receptor (CD88), PE (Clone 20/70)(rat IgG2b)
Reactivity
Mouse
Applications
FC/FACS, IF, BL
Pricing
$265/0.05 mg | $975/5x0.05 mg
Flow Cytometry (FC/FACS)
(Blank control: Hela. Primary Antibody (green line): Rabbit Anti-LRFN4 antibody (bs-11089R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 0.2 µg /test. Protocol The cells were incubated in 5 %BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. )
LRFN4, Polyclonal Antibody (Cat# AAA9233940)
Full Name
LRFN4 Polyclonal Antibody
Gene Names
LRFN4; SALM3; SALM3.; FIGLER6
Reactivity
Rat, Pig, Dog, Cow
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Flow Cytometric Analysis of PFA-fixed HeLa cells. GRAMD4 Mouse Monoclonal Antibody (PCRP-GRAMD4-1A10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red). )
GRAMD4, Monoclonal Antibody (Cat# AAA4381897)
Full Name
GRAMD4 (Transcription Factor) Mouse Monoclonal Antibody [Clone PCRP-GRAMD4-1A10]
Gene Names
GRAMD4; DIP; dA59H18.1; dJ439F8.1
Reactivity
Human
Predicted to react in Rat.
Predicted to react in Rat.
Applications
FC/FACS, IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Flow Cytometry (FC/FACS)
(Blank control:Hela. Primary Antibody (green line): Rabbit Anti- Synaptotagmin XII antibody (bs-11398R) Dilution: 2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1 µg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. )
Synaptotagmin XII, Polyclonal Antibody (Cat# AAA9234060)
Full Name
Synaptotagmin XII Polyclonal Antibody
Gene Names
SYT12; SYT11; sytXII
Reactivity
Rat, Pig, Dog
Applications
WB, IHC-P, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Blank control(blue):mouse spleen cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti- A4GALT antibody(bs-7588R), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. )
A4GALT, Polyclonal Antibody (Cat# AAA9238258)
Full Name
A4GALT Polyclonal Antibody
Gene Names
A4GALT; P1; PK; Gb3S; P(k); P1PK; A14GALT; A4GALT1
Reactivity
Rat, Pig, Dog
Applications
IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Wester Blot (WB)
(Sample: Embryo (Mouse) Lysate at 40 ug Primary: Anti- MC4 Receptor (bs-11417R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 52 kD )
MC4 Receptor, Polyclonal Antibody (Cat# AAA9234066)
Full Name
MC4 Receptor Polyclonal Antibody
Reactivity
Rat
Applications
WB, IHC-F, IF, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-IFN-gamma receptor 1 antibody GR20 (MBS488632). Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of GR20 (MBS488632, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IFN-gamma receptor 1, Monoclonal Antibody (Cat# AAA488633)
Full Name
Anti-IFN-gamma receptor 1 [GR20]
Gene Names
Ifngr1; Ifgr; CD119; Ifngr; Nktar; IFN-gammaR
Reactivity
Mouse
Applications
BL, NEU, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-IL2R beta antibody 5H4 (MBS488672) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 5H4 (MBS488672) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488672, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
IL2R beta, Monoclonal Antibody (Cat# AAA488673)
Full Name
Anti-IL2R beta [5H4]
Gene Names
Il2rb; p70; CD122; IL15Rbeta; Il-2Rbeta; IL-15Rbeta; Il-2/15Rbeta
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-9 antibody RM9A4 (MBS488654). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of RM9A4 (, pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with . After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.)
IL-9, Monoclonal Antibody (Cat# AAA488654)
Full Name
Anti-IL-9 [RM9A4]
Gene Names
Il9; P40; Il-9
Reactivity
Mouse
Applications
EIA, FC/FACS
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CCR9 antibody 9B1 (MBS488766) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 9B1 (MBS488766) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488766, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
CCR9, Monoclonal Antibody (Cat# AAA488767)
Full Name
Anti-CCR9 [9B1]
Gene Names
Ccr9; Cmkbr10; GPR-9-6; A130091K22Rik
Reactivity
Mouse
Applications
FC/FACS, NEU
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
