Western Blot (WB)
(Western Blot using anti-CCRL2 antibody (MBS488610) Mouse thymus (A) and mouse brain (B) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of CCRL2 (MBS488610) at 0.5ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Rat anti-Mouse CCRL2 Monoclonal Antibody | anti-CCRL2 antibody
Anti-CCRL2 [BZ5B8]
Western Blot (WB)
(Western Blot using anti-CCRL2 antibody (MBS488610) Mouse thymus (A) and mouse brain (B) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of CCRL2 (MBS488610) at 0.5ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-CCRL2 antibody (MBS488610) Mouse thymus (A) and mouse brain (B) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of CCRL2 (MBS488610) at 0.5ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CCRL2 antibody BZ5B8 (B467) (MBS488610) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of BZ5B8 (MBS488610) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488610, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CCRL2 antibody BZ5B8 (B467) (MBS488610) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of BZ5B8 (MBS488610) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488610, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometry using the anti-CCRL2 antibody BZ5B8 (MBS488610). RAW264.7 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG1 version of BZ5B8 (MBS488610, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow cytometry using the anti-CCRL2 antibody BZ5B8 (MBS488610). RAW264.7 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG1 version of BZ5B8 (MBS488610, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide/KLH conjugate. The immunizing amino-terminal mCCRL2 peptide with the sequence NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1-24 of mCCRL2, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).
Note on publication: Describe the original generation of the anti-mCCRL2 mAbs BZ5B8 and BZ2E3 and verification of their binding specificity for murine CCRL2 by an ELISA-based assay. The antibodies were then used as part of the study which suggested that mast cell-expressed orphan receptor CCRL2 bound chemerin and was required for optimal induction of IgE-mediated passive cutaneous anaphylaxis.
NCBI and Uniprot Product Information
Uniprot Description
CCRL2: Receptor for CCL19 and chemerin/RARRES2. Does not appear to be a signaling receptor, but may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptors. Plays a critical role for the development of Th2 responses. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1
Cellular Component: integral to membrane; integral to plasma membrane; membrane; plasma membrane
Molecular Function: C-C chemokine receptor activity; CCR chemokine receptor binding; chemokine receptor activity; chemokine receptor binding; G-protein coupled receptor activity; signal transducer activity
Biological Process: chemotaxis; G-protein coupled receptor protein signaling pathway; inflammatory response; signal transduction
Research Articles on CCRL2
Similar Products
Product Notes
The CCRL2 ccrl2 (Catalog #AAA488611) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-CCRL2 [BZ5B8] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CCRL2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Functional Assay (FA). This antibody has been used in various FACS analyses, for instance, to confirm that it is only specific to mCCRL2-hemagglutinin (HA)/L1.2 transfectants and that peritoneal macrophages treated with LPS, TNFalpha, IFN-gamma, or poly:IC up-regulate mCCRL2 protein expression (Zabel et al., 2008). Of note, this antibody binding to mouse peritoneal mast cells could be inhibited by chemerin, a protein ligand for signaling receptor CMKLR1 (Zabel et al., 2008). This anti-CCRL2 antibody can be used in wide variety of assays to study leukocytes and immune response. For example, this antibody has been used to examine the expression, regulation, and function of atypical chemerin receptor CCRL2 on endothelial cells (Monnier et al., 2012) and to delineate how the chemoattractant chemerin suppresses melanoma by recruiting natural killer cell antitumor defenses (Pachynski et al., 2012). Researchers should empirically determine the suitability of the CCRL2 ccrl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCRL2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
