Flow Cytometry (FC/FACS)
(Balb/c peritoneal macrophages (left) or bone marrow (Gr-1 gate)(right) were stained with anti-C5a receptor (CD88)(clone: 20/70)(filled histogram) or rat IgG2b isotype control (open histogram).)
Rat anti-Mouse CD8a Monoclonal Antibody | anti-CD8a antibody
Anti-Mouse CD8a (Ly 2), APC (Clone CT-CD8a)(rat IgG2a)
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunohistochemistry
Synonyms
CD8a; Monoclonal Antibody; Anti-Mouse CD8a (Ly 2); APC (Clone CT-CD8a)(rat IgG2a); anti-CD8a antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a
Clone Number
CT-CD8a
Specificity
Mouse CD8a (Ly 2; Lyt 2)
Form/Format
APC conjugated IgG buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5mg/ml.
Applicable Applications for anti-CD8a antibody
Flow Cytometry (FC/FACS), Vivo Assay, Immunohistochemistry-Frozen (IHC-F), Immunohistochemistry-Paraffin (IHC-P)
Conjugation
Allophycocyanin (APC)
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE
Flow Cytometry (FC/FACS)
(Balb/c peritoneal macrophages (left) or bone marrow (Gr-1 gate)(right) were stained with anti-C5a receptor (CD88)(clone: 20/70)(filled histogram) or rat IgG2b isotype control (open histogram).)
Flow Cytometry (FC/FACS)
(Balb/c peritoneal macrophages (left) or bone marrow (Gr-1 gate)(right) were stained with anti-C5a receptor (CD88)(clone: 20/70)(filled histogram) or rat IgG2b isotype control (open histogram).)
Testing Data
(CL7588F (CD88 FITC) and CL8991PE (Gr-1 PE) staining on C57BL/6 mouse bone marrow.)
Testing Data
(CL7588F (CD88 FITC) and CL8991PE (Gr-1 PE) staining on C57BL/6 mouse bone marrow.)
Related Product Information for anti-CD8a antibody
anti-mouse CD8a antigen monoclonal antibody binds to a protein of approximately 30,000 daltons found on 85% of mouse thymocytes, mouse cytotoxic/suppressor T cells and their precursors. It does not bind to mouse helper/inducer T cells. This antibody is not strain specific and can be used with all commonly used laboratory mice.
References
1. Tomonari, K. and Spencer, S. 1990 Epitope-specific binding of CD8 regulates activation of T cells and induction of cytotoxicity. International Immunolgy 2(12):1189-1194.
2. Whiteland, Joanne L., et al. 1995 Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. Journal of Histochemistry & Cytochemistry 43.3: 313-320.
2. Whiteland, Joanne L., et al. 1995 Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. Journal of Histochemistry & Cytochemistry 43.3: 313-320.
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Product Notes
The CD8a (Catalog #AAA524523) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD8a (Ly 2), APC (Clone CT-CD8a)(rat IgG2a) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD8a can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Vivo Assay, Immunohistochemistry-Frozen (IHC-F), Immunohistochemistry-Paraffin (IHC-P). Researchers should empirically determine the suitability of the CD8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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