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Reactivity
Gene Name
Isotype
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Application
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3095 results for "ubiquitin-binding-protein" - showing 50-100
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant SQSTM1 protein (red) or empty vector (blue) stained using SQSTM1 antibody)
SQSTM1, Monoclonal Antibody (Cat# AAA833418)
Full Name
SQSTM1 antibody
Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B; FTDALS3
Applications
FC/FACS, IF, WB
Purity
SQSTM1 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells, using Ubiquilin1 Antibody (Center). MBS9204638 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
Ubiquilin1, Polyclonal Antibody (Cat# AAA9204638)
Full Name
Ubiquilin1 Antibody (Center)
Gene Names
UBQLN1; DA41; DSK2; UBQN; XDRP1; PLIC-1
Reactivity
Reactivity: Human, Mouse
Predicted: Rat
Predicted: Rat
Applications
EIA, IHC-P, WB, IF
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of 293 cells, using Phospho-MDM2-S166 antibody. 293 cells were treated by Hydroxyurea (4mM) for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
MDM2-S166, Antibody (Cat# AAA125489)
Full Name
Phospho-MDM2-S166 Polyclonal Antibody
Gene Names
MDM2; HDMX; hdm2; ACTFS
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of VHL expression in whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
VHL, Polyclonal Antibody (Cat# AAA9601868)
Full Name
VHL Antibody
Gene Names
VHL; RCA1; VHL1; pVHL; HRCA1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant RPN1 protein (red) or empty vector (blue) stained using RPN1 antibody)
RPN1, Monoclonal Antibody (Cat# AAA833641)
Full Name
RPN1 antibody
Gene Names
RPN1; OST1; RBPH1
Applications
FC/FACS, IF, WB
Purity
RPN1 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Activity Data
(Figure. The binding activity of NOS2 with UCHL5.Nitric oxide synthase 2, inducible (NOS2) is a member of Nitric oxide synthases (NOSs) family. Nitric oxide synthases (NOSs) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. NO is an important cellular signaling molecule. It helps modulate vascular tone, insulin secretion, airway tone, and peristalsis, and is involved in angiogenesis and neural development. Besides, Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5) has been identified as an interactor of NOS2, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse NOS2 and recombinant mouse UCHL5. Briefly, NOS2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCHL5-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-NOS2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of NOS2 and UCHL5 was shown in Figure 1, and this effect was in a dose dependent manner.)
Nitric Oxide Synthase 2, Inducible (NOS2), Active Protein (Cat# AAA2105120)
Full Name
Active Nitric Oxide Synthase 2, Inducible (NOS2)
Gene Names
Nos2; iNOS; Nos-2; Nos2a; i-NOS; NOS-II; MAC-NOS
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$250/0.01 mg | $540/0.05 mg | $840/0.1 mg | $1,045/0.2 mg | $2,040/0.5 mg | $3,020/1 mg | $7,385/5 mg
Western Blot (WB)
(Western blot analysis of RBBP6 using HuvEc whole cell lysates)
RBBP6, Polyclonal Antibody (Cat# AAA9610169)
Full Name
RBBP6 Antibody
Gene Names
RBBP6; PACT; MY038; P2P-R; RBQ-1; SNAMA
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant SQSTM1 protein (red) or empty vector (blue) stained using SQSTM1 antibody)
SQSTM1, Monoclonal Antibody (Cat# AAA834815)
Full Name
SQSTM1 antibody
Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B; FTDALS3
Applications
FC/FACS, IF, WB
Purity
SQSTM1 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Immunohistochemistry (IHC)
(Sample Type :Mouse tongue tissuePrimary Antibody Dilution :1:100Secondary Antibody :Anti-rabbit-Cy3Secondary Antibody Dilution :1:500Color/Signal Descriptions :Red: POU2F3Gene Name :POU2F3Submitted by :Dr. Hong Wang, Monell Chemical Senses Center)
POU2F3, Polyclonal Antibody (Cat# AAA3201005)
Full Name
POU2F3 antibody - N-terminal region
Gene Names
POU2F3; PLA1; OCT11; PLA-1; Epoc-1; OCT-11; OTF-11; Skn-1a
Reactivity
Dog, Guinea Pig, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
IHC, WB
Purity
Protein A purified
Pricing
$390/0.1 mL | $1,750/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from A549 cell line and human plasma tissue lysate(from left to right), using CLU Antibody (N-term). MBS9215265 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
CLU, Polyclonal Antibody (Cat# AAA9215265)
Full Name
CLU Antibody (N-term)
Gene Names
CLU; CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2
Reactivity
Human, mouse, rat
Applications
FC, WB, IHC-P, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of BRAP using LOVO whole cell lysates)
BRAP, Polyclonal Antibody (Cat# AAA9609606)
Full Name
BRAP Antibody
Gene Names
BRAP; IMP; BRAP2; RNF52
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9610312 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
OTUB1, Polyclonal Antibody (Cat# AAA9610312)
Full Name
OTUB1 Antibody
Gene Names
OTUB1; OTB1; OTU1; HSPC263
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
SDS-Page
(SDS-PAGE Analysis Purified BAP1 Mouse Monoclonal Antibody (BAP1/2667). Confirmation of Purity and Integrity of Antibody.)
BAP1 (BRCA1 Associated Protein 1), Monoclonal Antibody (Cat# AAA4381358)
Full Name
BAP1 (BRCA1 Associated Protein 1)
Gene Names
BAP1; UCHL2; hucep-6; HUCEP-13
Reactivity
Human
Applications
EIA
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$375/0.1 mg (With BSA & Azide at 0.2mg/mL) | $375/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,625/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,625/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using OTUB2 Antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: Hela; )
OTUB2, Polyclonal Antibody (Cat# AAA9602190)
Full Name
OTUB2 Antibody
Gene Names
OTUB2; OTB2; OTU2; C14orf137
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot detection of KAP1 / TIF1 beta in 293T and HepG2 cell lysates using KAP1 / TIF1 beta mouse mAb (1:1000 diluted).Observed band size: 110KDa.)
KAP1/TIF1beta, Monoclonal Antibody (Cat# AAA475028)
Full Name
Anti-KAP1/TIF1beta Mouse mAb
Gene Names
TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
Reactivity
Human
Applications
WB, ICC, IHC, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with BAP1 Mouse Monoclonal Antibody (BAP1/2432).)
BAP1 (BRCA1 Associated Protein 1), Monoclonal Antibody (Cat# AAA4380726)
Full Name
BAP1 (BRCA1 Associated Protein 1)
Gene Names
BAP1; UCHL2; hucep-6; HUCEP-13
Reactivity
Human. Others not known.
Applications
Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot detection of UHRF1 in Mouse heart, 293T, HelaNE and Hela cell lysates using UHRF1 mouse mAb (1:1000 diluted).Predicted band size: 90KDa.Observed band size: 90KDa.)
UHRF1, Monoclonal Antibody (Cat# AAA475085)
Full Name
Anti-UHRF1 (N-term) Mouse mAb
Gene Names
UHRF1; Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-SQSTM1/p62 (Ser403) using HepG2 whole cell lysates)
SQSTM1/p62, Polyclonal Antibody (Cat# AAA9604449)
Full Name
Phospho-SQSTM1/p62 (Ser403) Antibody
Gene Names
SQSTM1; p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(Human Muscle )
POU2F3, Polyclonal Antibody (Cat# AAA3201004)
Full Name
POU2F3 antibody - N-terminal region
Gene Names
POU2F3; PLA1; OCT11; PLA-1; Epoc-1; OCT-11; OTF-11; Skn-1a
Reactivity
Dog, Guinea Pig, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunofluorescence (IF)
(MBS9601938 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
MDM2, Polyclonal Antibody (Cat# AAA9601938)
Full Name
MDM2 Antibody
Gene Names
MDM2; HDMX; hdm2; ACTFS
Reactivity
Human, Mouse, Monkey
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CBLC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
CBLC, Polyclonal Antibody (Cat# AAA9128673)
Full Name
CBLC Polyclonal Antibody
Gene Names
CBLC; CBL-3; RNF57; CBL-SL
Reactivity
Human, Mouse
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using MDM2 (Phospho-Ser166) antibody)
MDM2, Polyclonal Antibody (Cat# AAA5309638)
Full Name
MDM2 antibody
Gene Names
MDM2; HDMX; hdm2; ACTFS
Applications
IF, IHC, WB
Purity
Purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CDC34 Mouse Monoclonal Antibody (CPTC-CDC34-2).)
Cell Division Cycle 34 homolog, Monoclonal Antibody (Cat# AAA4380753)
Full Name
Cell Division Cycle 34 homolog
Gene Names
CDC34; UBC3; UBCH3; UBE2R1; E2-CDC34
Reactivity
Human. Others not tested.
Applications
Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot analysis of UBE2D3 expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
UBE2D3, Polyclonal Antibody (Cat# AAA9603797)
Full Name
UBE2D3 Antibody
Gene Names
UBE2D3; UBC4/5; UBCH5C; E2(17)KB3
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(Rabbit Anti-Lrp2bp antibodyFormalin Fixed Paraffin Embedded Tissue: Human KidneyPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)
Lrp2bp, Polyclonal Antibody (Cat# AAA3206425)
Full Name
Lrp2bp antibody - middle region
Gene Names
Lrp2bp; RGD1305896
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB)
(Western blot analysis of TRAF3 expression in NIH-3T3 cells.The lane on the left is treated with the antigen-specific peptide.)
TRAF3, Polyclonal Antibody (Cat# AAA9601552)
Full Name
TRAF3 Antibody
Gene Names
TRAF3; CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp; RNF118
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using UBFD1 antibody.)
UBFD1, Polyclonal Antibody (Cat# AAA9606050)
Full Name
UBFD1 Antibody
Gene Names
UBFD1; UBPH
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of TDP43 using HT-29 whole cell lysates)
TDP43, Polyclonal Antibody (Cat# AAA9604433)
Full Name
TDP43 Antibody
Gene Names
TARDBP; ALS10; TDP-43
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using UHRF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
UHRF1, Polyclonal Antibody (Cat# AAA127391)
Full Name
UHRF1 Polyclonal Antibody
Gene Names
UHRF1; Np95; hNP95; ICBP90; RNF106; hUHRF1; huNp95
Reactivity
Human
Applications
WB, IHC, IF, IP
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant RPN1 protein (red) or empty vector (blue) stained using RPN1 antibody)
RPN1, Monoclonal Antibody (Cat# AAA833396)
Full Name
RPN1 antibody
Gene Names
RPN1; OST1; RBPH1
Applications
FC/FACS, IHC, WB
Purity
RPN1 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Immunohistochemistry (IHC)
(MBS9608015 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
UBE2C, Polyclonal Antibody (Cat# AAA9608015)
Full Name
UBE2C Antibody
Gene Names
UBE2C; UBCH10; dJ447F3.2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Recommended SQSTM1 Antibody Titration: 0.2-1 ug/ml)
Sequestosome 1, Polyclonal Antibody (Cat# AAA5300080)
Full Name
Sequestosome 1 antibody
Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B; FTDALS3
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity purified
Pricing
$605/0.1 mL | $2,710/5x0.1 mL
Immunohistochemistry (IHC)
(Rabbit Anti-HNRNPU AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Testis TissueObserved Staining: Cytoplasm and nucleus in spermatogonia and spermatocytesPrimary Antibody Concentration: N/AOther Working Concentrations: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
HNRNPU, Polyclonal Antibody (Cat# AAA3205292)
Full Name
HNRNPU antibody - N-terminal region
Gene Names
HNRNPU; SAFA; HNRPU; SAF-A; U21.1; pp120; EIEE54; GRIP120; hnRNP U; HNRNPU-AS1
Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Rabbit, Rat
Applications
WB, IHC
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 47.7 kDa SQSTM1 protein using Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-637). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-637) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 47.7 kDa. Other Band(s): ~60 in Jurkat.)
SQSTM1, Polyclonal Antibody (Cat# AAA809653)
Full Name
SQSTM1 Antibody: ATTO 390
Gene Names
SQSTM1; p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
Reactivity
Human; Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Activity Data
(Figure. The apoptosis of A549 cells by TNFalpha was inhibited by TNFRSF1B.Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B), also known as tumor necrosis factor receptor 2 (TNFR2) and CD120b, is a membrane receptor that binds tumor necrosis factor-alpha (TNFalpha). This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. TNFRSF1B can inhibit cell apoptosis which induced by TNFalpha. Briefly, A549 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM including 1mug/mL TNFalpha and various concentrations of recombinant human TNFRSF1B. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate at 37 degree C for 1-4 hours. Apoptosis of A549 cells had been inhibit after incubation with TNFRSF1B for 96h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant TNFRSF1B for 96h. The result was shown in Figure 2. It was obvious that TNFRSF1B significantly suppress cell apoptosis induced by TNFalpha.(A) A549 cells cultured in DMEM contain 1mug/mL TNFalpha and 0.1ng/mL TNFRSF1B for 96h; (B) A549 cells cultured in DMEM only contain 1mug/mL TNFalpha for 96h.)
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A), Active Protein (Cat# AAA2105140)
Full Name
Active Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A)
Gene Names
TNFRSF1A; FPF; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$185/0.01 mg | $365/0.05 mg | $560/0.1 mg | $685/0.2 mg | $1,330/0.5 mg | $1,975/1 mg | $4,855/5 mg
Western Blot (WB)
(Western blot analysis of Cdc34 using HepG2 whole cell lysates)
CDC34, Polyclonal Antibody (Cat# AAA9608838)
Full Name
CDC34 Antibody
Gene Names
CDC34; UBC3; UBCH3; UBE2R1; E2-CDC34
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from HT29, Hela and 293 cells using MDM2(Ab-166) Antibody.)
MDM2, Polyclonal Antibody (Cat# AAA9405515)
Full Name
MDM2 (Ab-166) Antibody
Gene Names
MDM2; HDMX; hdm2; ACTFS
Reactivity
Human
Applications
WB, IHC, IF
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5 % SDS PAGE gel and TAP antibody at a dilution of 1:1000)
TAP, Polyclonal Antibody (Cat# AAA835674)
Full Name
TAP antibody
Applications
IF, ICC, WB
Purity
TAP antibody was purified by Protein A chromatography
Pricing
$700/0.1 mL | $3,130/5x0.1 mL
Immunohistochemistry (IHC)
(MBS9607830 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
TRAF3, Polyclonal Antibody (Cat# AAA9607830)
Full Name
TRAF3 Antibody
Gene Names
TRAF3; CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp; RNF118
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western Blot Analysis of human HepG2 cell lysate using MDM2 Mouse Monoclonal Antibody (SMP14).)
MDM2, Monoclonal Antibody (Cat# AAA4381084)
Full Name
MDM2
Gene Names
MDM2; HDMX; hdm2; ACTFS
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot analysis on COS7 cell lysate using MDM2 Antibody. The lane on the left is treated with the antigen-specific peptide.)
MDM2, Polyclonal Antibody (Cat# AAA9600146)
Full Name
MDM2 Antibody
Gene Names
MDM2; HDMX; hdm2; ACTFS
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, IF, ICC, IP, EIA
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis UBQLN2 using HeLa whole cell lysates)
UBQLN2, Polyclonal Antibody (Cat# AAA9610294)
Full Name
UBQLN2 Antibody
Gene Names
UBQLN2; DSK2; ALS15; CHAP1; N4BP4; PLIC2; HRIHFB2157
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of TOPRS expression in HEK293 cells lysate)
TOPRS, Polyclonal Antibody (Cat# AAA9603989)
Full Name
TOPRS Antibody
Gene Names
TOPORS; LUN; RP31; P53BP3; TP53BPL
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9606054 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
UBE2D2, Polyclonal Antibody (Cat# AAA9606054)
Full Name
UBE2D2 Antibody
Gene Names
UBE2D2; UBC4; PUBC1; UBCH4; UBC4/5; UBCH5B; E2(17)KB2
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of ZRANB1 Antibody (C-term) in K562 cell line lysates (35ug/lane). ZRANB1 (arrow) was detected using the purified Pab.)
ZRANB1, Polyclonal Antibody (Cat# AAA9203740)
Full Name
ZRANB1 Antibody (C-term)
Gene Names
ZRANB1; TRABID
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
WB, EIA, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Otubain 1 (OTUB1), Polyclonal Antibody (Cat# AAA2005817)
Full Name
Polyclonal Antibody to Otubain 1 (OTUB1)
Gene Names
Otub1; AI850305
Reactivity
Mouse
Applications
ICC, IHC, EIA, WB
Purity
Affinity Chromatography
Pricing
$180/0.1 mL | $220/0.2 mL | $385/0.5 mL | $475/1 mL | $1,320/5x1 mL
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant PSMC3 protein (red) or empty vector (blue) stained using PSMC3 antibody)
PSMC3, Monoclonal Antibody (Cat# AAA838383)
Full Name
PSMC3 antibody
Gene Names
PSMC3; TBP1
Applications
FC/FACS, IF, IHC, WB
Purity
PSMC3 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Activity Data
(Figure. The binding activity of TNFRSF1B with TNFa.Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B), also known as tumor necrosis factor receptor 2 (TNFR2) and CD120b, is a membrane receptor that binds tumor necrosis factor-alpha (TNFalpha). This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Besides, Tumor Necrosis Factor Alpha (TNFa) has been identified as an interactor of TNFRSF1B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNFRSF1B and recombinant human TNFa. Briefly, TNFRSF1B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF1B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF1B and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.)
Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B), Active Protein (Cat# AAA2105142)
Full Name
Active Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)
Gene Names
TNFRSF1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $650/0.2 mg | $1,260/0.5 mg | $1,870/1 mg | $4,595/5 mg
Western Blot (WB)
(Western blot analysis of extracts of 3T3-L1 cells, using SQSTM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
SQSTM1, Polyclonal Antibody (Cat# AAA9131400)
Full Name
SQSTM1 Polyclonal Antibody
Gene Names
SQSTM1; p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunohistochemistry (IHC)
(Anti-SQSTM1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
p62 / SQSTM1, Polyclonal Antibody (Cat# AAA244060)
Full Name
Rabbit Polyclonal to Human p62 / SQSTM1
Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B
Reactivity
Human, Mouse, Rat
Applications
IHC - Paraffin, WB, EIA
Purity
Immunoaffinity Purified
Pricing
$705/0.05 mg | $3,120/5x0.05 mg
