anti-Human KAP1/TIF1beta Monoclonal Antibody | anti-KAP1/TIF1beta antibody

Anti-KAP1/TIF1beta Mouse mAb

Cat. Num. AAA475028

• Gene Names: TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
• Purity: Affinity Purified
• Synonyms: KAP1/TIF1beta; Monoclonal Antibody; Anti-KAP1/TIF1beta Mouse mAb; E3 SUMO protein ligase TRIM28; E3 SUMO-protein ligase TRIM28; FLJ29029; KAP 1; KAP-1; KRAB associated protein 1; KRAB interacting protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP 1; KRIP-1; KRIP1; Nuclear corepressor KAP 1; Nuclear corepressor KAP-1; RING finger protein 96; RNF96; TF1B; TIF1 beta; TIF1-beta; TIF1B; TIF1B_HUMAN; Transcription intermediary factor 1 beta; Transcription intermediary factor 1-beta; TRIM28; Tripartite motif containing 28; tripartite motif containing protein 28; Tripartite motif-containing protein 28; anti-KAP1/TIF1beta antibody

• Host: Mouse; Source: Human
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4E1-D12-F8
• Purity/Purification: Affinity Purified
• Form/Format: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%, glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 835

• Applicable Applications for anti-KAP1/TIF1beta antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000; ICC: 1:100
• Immunogen: Purified recombinant human KAP1 / TIF1 beta protein fragments expressed in E Coli.
• Sensitivity: This antibody detects endogenous levels of KAP1 / TIF1 beta and does not cross-react with related proteins.
• Sequence Similarities: Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 bromo domain. Contains 1 PHD-type zinc finger. Contains 1 RING-type zinc finger.
• Subunit Structure: Oligomer; the RBCC domain homotrimerizes and interacts with one molecule of KRAB to form the KRAB-KAP1 corepressor complex. Binding to a KRAB domain is an absolute requirement for silencing gene expression. Interacts with CEBPB and NR3C1 By similarity. Interacts with a number of KRAB-ZFP proteins including ZNF10, ZFP53, ZFP68, ZNF382 and ZNF256. Interacts with NCOR1, NR3C1 and CHD3. Interacts with CEBPB (via the RING-type and PHD-type zinc fingers). Component of a ternary complex that includes TRIM28, a HP1 protein (CBX1, CBX3 OR CBX5), a KRAB domain-containing protein, and DNA. Interacts with CBX5 (via the PxVxL motif); the interaction occurs in interphase nuclei and competes for binding POGZ. Interacts with POGZ; the interaction competes for interaction with CBX5. Interacts with SETDB1; the interaction is enhanced by KAP1 sumoylation, stimulates SETB1 histone methyltransferase activity and gene silencing. Interacts (via the PHD-type zinc finger) with UBE2I; the interaction is required for sumoylation and repressor activity. Component of the TRIM28/KAP1-ERBB4-MDM2 complex involved in connecting growth factor and DNA damage responses. Interacts directly with ERBB4; the interaction represses ERBB4-mediated transcription activity. Interacts with MDM2; the interaction contributes to p53/TP53 inactivation. Component of the TRIM28/KAP1-MDM2-p53/TP53; involved in regulating p53/TP53 stabilization and activity. Interacts (via the leucine zipper alpha helical coiled-coil) with E2F1 (central region); the interaction inhibits E2F1 acetylation and transcriptional activity. Interacts with PPP1CA; the interaction dephosphorylates TRIM28 at Ser-824 and forms a complex at the p21 promoter site. Interacts with PPP1CB; the interaction is weak but is increased on dephophorylation at Ser-824. Interacts with FES/FPS. Interacts with SMARCAD1. Interacts with, and sumoylates IRF7.
• Subcellular Location: Nucleus

Western Blot (WB)

(Western blot detection of KAP1 / TIF1 beta in 293T and HepG2 cell lysates using KAP1 / TIF1 beta mouse mAb (1:1000 diluted).Observed band size: 110KDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-KAP1 / TIF1 beta mouse mAb (dilution 1:100).)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using KAP1 / TIF1 beta mouse mAb.)

Immunohistochemistry (IHC)

(IHC of paraffin-embedded human Spleen using anti-KAP1 / TIF1 beta diluted 1/500-1/1000.)

Related Product Information for anti-KAP1/TIF1beta antibody

Entrez Summary: The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

NCBI and Uniprot Product Information

• NCBI GI #: 5032179
• NCBI GeneID: 10155
• NCBI Accession #: NP_005753.1
• NCBI GenBank Nucleotide #: NM_005762
• UniProt Accession #: Q13263; O00677; Q7Z632; Q93040; Q96IM1
• Molecular Weight: 110
• NCBI Official Full Name: transcription intermediary factor 1-beta
• NCBI Official Synonym Full Names: tripartite motif containing 28
• NCBI Official Symbol: TRIM28
• NCBI Official Synonym Symbols: KAP1; TF1B; RNF96; TIF1B; PPP1R157
• NCBI Protein Information: transcription intermediary factor 1-beta
• UniProt Protein Name: Transcription intermediary factor 1-beta
• UniProt Gene Name: TRIM28
• UniProt Synonym Gene Names: KAP1; RNF96; TIF1B; TIF1-beta; KAP-1; KRIP-1

NCBI Description

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

Uniprot Description

TIF1B: an atypical protein kinase that functions as a transcriptional coactivator. Mediates transcriptional regulation by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors. Binds to the negative regulatory domain of c-Myb and negatively regulates the c-Myb-dependent trans-activation. Localizes to the nucleus and is thought to associate with specific chromatin regions. Phosphorylated in an ATM-dependent manner following double-strand breaks. Its phosphorylation in response to DNA damage leads to chromatin relaxation. A member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Protein type: ATYPICAL group; EC 6.3.2.-; Kinase, protein; Ligase; Nuclear receptor co-regulator; Protein kinase, atypical; TIF1 family; Transcription, coactivator/corepressor; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 19q13.43

Cellular Component: nucleoplasm; nucleus

Molecular Function: chromatin binding; DNA binding; protein binding; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: DNA repair; epithelial to mesenchymal transition; innate immune response; negative regulation of transcription, DNA-dependent; positive regulation of DNA repair; positive regulation of transcription factor import into nucleus; positive regulation of transcription, DNA-dependent; protein oligomerization; protein sumoylation; Ras protein signal transduction; transcription initiation from RNA polymerase II promoter