Rabbit anti-Human, Mouse TOPRS Polyclonal Antibody | anti-TOPRS antibody

TOPRS Antibody

Cat. Num. AAA9603989

• Gene Names: TOPORS; LUN; RP31; P53BP3; TP53BPL
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: TOPRS; Polyclonal Antibody; TOPRS Antibody; AW105885; E3 ubiquitin protein ligase Topors; E3 ubiquitin-protein ligase Topors; LUN; p53 binding protein 3; p53 BP3/LUN; p53-binding protein 3; p53BP3; p53BP3/LUN; Retinitis pigmentosa 31; RP31; SUMO1 protein E3 ligase Topors; SUMO1-protein E3 ligase Topors; Topoisomerase I binding arginine/serine rich; Topoisomerase I binding arginine/serine rich protein; Topoisomerase I binding protein; Topoisomerase I binding RING finger protein; Topoisomerase I-binding arginine/serine-rich protein; Topoisomerase I-binding RING finger protein; Topoisomerase I-binding RS protein; Topors; TOPRS_HUMAN; TP53BPL; Tumor protein p53 binding protein; Tumor suppressor p53 binding protein 3; Tumor suppressor p53-binding protein 3; anti-TOPRS antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TOPRS antibody detects endogenous levels of total TOPRS
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 980

• Applicable Applications for anti-TOPRS antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide
• Subcellular Location: Nucleus. Nucleus > PML Body. Localizes to discrete nuclear foci which partly overlap with PML nuclear bodies. Targeted to PML nuclear bodies upon DNA damage.
• Tissue Specificity: Expressed at highest levels in testis and at lower levels in adrenal gland, bone marrow, brain, colon, heart, kidney, liver, muscle, ovary, pancreas, placenta, prostate, skeletal muscle, skin, small intestine, spleen, stomach, testis, thymus, thyroid and uterus. Expressed in the alveolar epithelium of the lung. Expression is commonly decreased in colon adenocarcinomas and lung cancers.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Similarity: Pig (80%), Bovine (100%), Horse (80%), Sheep (100%), Rabbit (100%), Dog (90%), Chicken (88%)
• Conjugation: Unconjugated

Western Blot (WB)

(Western blot analysis of TOPRS expression in HEK293 cells lysate)

Related Product Information for anti-TOPRS antibody

Function: Functions as an E3 ubiquitin-protein ligase and as an E3 SUMO1-protein ligase. Probable tumor suppressor involved in cell growth, cell proliferation and apoptosis that regulates p53/TP53 stability through ubiquitin-dependent degradation. May regulate chromatin modification through sumoylation of several chromatin modification-associated proteins. May be involved in DNA damage-induced cell death through IKBKE sumoylation.
Subunit Structure: Interacts with PARK7/DJ-1 (By similarity). Interacts with TOP1. Interacts with p53/TP53; can both ubiquitinate and sumoylate p53/TP53. Interacts with the SUMO1 conjugating enzyme UBE2I. Interacts with SUMO1. Interacts with NKX3-1; polyubiquitinates NKX3-1 and induces its proteasomal degradation. Interacts with SIN3A; sumoylates SIN3A. Interacts with IKBKE; induced by DNA damage.
Post-translational Modifications: Phosphorylation at Ser-98 regulates the E3 ubiquitin-protein ligase activity but not the SUMO1-protein ligase activity. Phosphorylation at Ser-718 increases the E3 ubiquitin-protein ligase activity versus the SUMO1-protein ligase activity resulting in increased p53/TP53 ubiquitination and degradation. Sumoylated.

NCBI and Uniprot Product Information

• NCBI GI #: 307344673
• NCBI GeneID: 10210
• NCBI Accession #: NP_001182551.1
• NCBI GenBank Nucleotide #: NM_001195622.1
• UniProt Accession #: Q9NS56; O43273; Q6P987; Q9NS55; Q9UNR9
• Molecular Weight: Predicted: 120 kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase Topors isoform 2
• NCBI Official Synonym Full Names: TOP1 binding arginine/serine rich protein
• NCBI Official Symbol: TOPORS
• NCBI Official Synonym Symbols: LUN; RP31; P53BP3; TP53BPL
• NCBI Protein Information: E3 ubiquitin-protein ligase Topors
• UniProt Protein Name: E3 ubiquitin-protein ligase Topors
• UniProt Gene Name: TOPORS
• UniProt Synonym Gene Names: LUN; TP53BPL; p53-binding protein 3; p53BP3

NCBI Description

This gene encodes a nuclear protein which is serine and arginine rich, and contains a RING-type zinc finger domain. It is highly expressed in the testis, and functions as an ubiquitin-protein E3 ligase. Mutations in this gene are associated with retinitis pigmentosa type 31. Alternatively spliced transcript variants, encoding different isoforms, have been observed for this locus. [provided by RefSeq, Sep 2010]

Uniprot Description

Functions as an E3 ubiquitin-protein ligase and as an E3 SUMO1-protein ligase. Probable tumor suppressor involved in cell growth, cell proliferation and apoptosis that regulates p53/TP53 stability through ubiquitin-dependent degradation. May regulate chromatin modification through sumoylation of several chromatin modification-associated proteins. May be involved in DNA damage-induced cell death through IKBKE sumoylation.