Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded malignant cells of human lung tissue (Fig. 1 & 2) was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0.)
Rabbit anti-Mouse, Human NOS II Polyclonal Antibody | anti-NOS2 antibody
NOS II (iNOS, Nitric Oxide Synthase, NOS 2) (APC)
WB: 0.5ug/ml
IP: 10ug per 500ug treated cell lysate
Applications are based on unconjugated antibody.
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded malignant cells of human lung tissue (Fig. 1 & 2) was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0.)
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded malignant cells of human lung tissue (Fig. 1 & 2) was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0.)
Immunoprecipitation (IP)
(Immunoprecipitation Analysis: Representative lot data. 10 ug of this antibody immunoprecipitated iNOS/NOSII from 0.5 mg of IFNgamma/LPS treated RAW264.7 cell lysate)
Immunoprecipitation (IP)
(Immunoprecipitation Analysis: Representative lot data. 10 ug of this antibody immunoprecipitated iNOS/NOSII from 0.5 mg of IFNgamma/LPS treated RAW264.7 cell lysate)
Western Blot (WB)
(Western blot analysis: IFNgamma/LPS untreated (lane 1) and treated (lane 2) RAW264.7 cell lysates were probed with Anti-iNOS/NOS II, NT (0.5ug/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.)
Western Blot (WB)
(Western blot analysis: IFNgamma/LPS untreated (lane 1) and treated (lane 2) RAW264.7 cell lysates were probed with Anti-iNOS/NOS II, NT (0.5ug/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.)
NCBI and Uniprot Product Information
NCBI Description
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Uniprot Description
iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase
Cellular Component: cortical cytoskeleton; extracellular space; perinuclear region of cytoplasm; cytoplasm; peroxisome; intracellular; vesicle membrane; cytosol; nucleus
Molecular Function: protein homodimerization activity; FAD binding; nitric-oxide synthase activity; metal ion binding; beta-catenin binding; cAMP-dependent protein kinase regulator activity; oxidoreductase activity; Hsp90 protein binding; actin binding; protein kinase binding; calmodulin binding; protein binding; NADPH-hemoprotein reductase activity; FMN binding; cadherin binding; iron ion binding; heme binding; nitric-oxide synthase binding; NADP binding; receptor binding
Biological Process: superoxide metabolic process; response to lipopolysaccharide; positive regulation of guanylate cyclase activity; positive regulation of killing of cells of another organism; positive regulation of vasodilation; ovulation from ovarian follicle; regulation of cell proliferation; defense response to bacterium; response to hypoxia; peptidyl-cysteine S-nitrosylation; arginine catabolic process; negative regulation of blood pressure; negative regulation of protein catabolic process; nitric oxide biosynthetic process; inflammatory response; regulation of protein kinase activity; regulation of insulin secretion; nitric oxide mediated signal transduction
Research Articles on NOS2
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Product Notes
The NOS2 nos2 (Catalog #AAA6490298) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NOS II (iNOS, Nitric Oxide Synthase, NOS 2) (APC) reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's NOS II can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunoprecipitation (IP). IHC-P: 1:50-1:2:00 WB: 0.5ug/ml IP: 10ug per 500ug treated cell lysate Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NOS2 nos2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOS II, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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