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10000 results for "Various" - showing 850-900


Western Blot (WB) (Western blot analysis of extracts of various cell lines, using LPCAT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

LPCAT1, Polyclonal Antibody (Cat# AAA9127857)

Full Name
LPCAT1 Polyclonal Antibody
Gene Names
LPCAT1; AYTL2; lpcat; PFAAP3
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunohistochemistry (IHC) (MBS9608464 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

ORAI1, Antibody (Cat# AAA9608464)

Full Name
ORAI1 Antibody
Gene Names
ORAI1; IMD9; TAM2; ORAT1; CRACM1; TMEM142A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Flow Cytometry (FC/FACS) (Flow Cytometry of human Ep-CAM on HT29 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Ep-CAM Monoclonal Antibody (VU-1D9).)

Ep-CAM /CD326, Monoclonal Antibody (Cat# AAA438368)

Full Name
Ep-CAM /CD326 (Epithelial Marker) Mouse Monoclonal Antibody
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human.
Does not react with Rat and Ferret
Applications
FC/FACS, IF
Purity
Purified Ab conjugated to PE
Pricing
$390/100 Tests | $1,710/5x100 Tests
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Monoclonal Antibody (FOXA1/1515).)

FOXA1/HNF3A, Monoclonal Antibody (Cat# AAA4380022)

Full Name
FOXA1/HNF3A
Gene Names
FOXA1; HNF3A; TCF3A
Reactivity
Human.
Does not react with rat. Others not known.
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunofluorescence (IF) (MBS9600840 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

TK, Polyclonal Antibody (Cat# AAA9600840)

Full Name
Phospho-TK (Ser13) Antibody
Gene Names
TK1; TK2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TCAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

TCAP, Polyclonal Antibody (Cat# AAA9126105)

Full Name
TCAP Polyclonal Antibody
Gene Names
TCAP; TELE; CMD1N; T-cap; LGMD2G; telethonin
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines with CPT1A Polyclonal Antibody)

CPT1A, Polyclonal Antibody (Cat# AAA2520073)

Full Name
CPT1A Antibody
Gene Names
CPT1A; CPT1; CPT1-L; L-CPT1
Reactivity
Human, Rat
Applications
WB, IF
Purity
Affinity purification
Pricing
$395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ATF6 antibody.)

ATF6, Polyclonal Antibody (Cat# AAA2518389)

Full Name
ATF6 Antibody
Gene Names
ATF6; ATF6A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Antigen affinity purification
Pricing
$395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL

CD147 / EMMPRIN / Neurothelin, Monoclonal Antibody (Cat# AAA438425)

Full Name
CD147 / EMMPRIN / Neurothelin Mouse Monoclonal Antibody
Gene Names
BSG; OK; 5F7; TCSF; CD147; EMMPRIN
Reactivity
Human
Applications
FC/FACS, IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
Western Blot (WB) (Western blot analysis of IR phosphorylation expression in Heatshock treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IR, Polyclonal Antibody (Cat# AAA9600871)

Full Name
Phospho-IR (Tyr1361) Antibody
Gene Names
INSR; HHF5; CD220
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9601120 at 1/200 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

PTEN, Polyclonal Antibody (Cat# AAA9601120)

Full Name
Phospho-PTEN (Ser370) Antibody
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using Phospho-CDK4-T172 pAb (MBS9126724) at 1:1000 dilution. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.)

CDK4-T172, Polyclonal Antibody (Cat# AAA9126724)

Full Name
Phospho-CDK4-T172
Gene Names
CDK4; CMM3; PSK-J3
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using PTGIR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

PTGIR, Polyclonal Antibody (Cat# AAA127986)

Full Name
PTGIR Polyclonal Antibody
Gene Names
PTGIR; IP; PRIPR
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western BLot (WB) (Western blot analysis of extracts from various samples, using SHIP1 Antibody.Lane 1: Mouse brain lysates;Lane 2: Mouse lung lysates;Lane 3: Mouse lung lysates treated with blocking peptide;)

SHIP1, Polyclonal Antibody (Cat# AAA9600189)

Full Name
SHIP1 Antibody
Gene Names
INPP5D; SHIP; SHIP1; SHIP-1; hp51CN; SIP-145; p150Ship
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9604994 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

CADM3, Polyclonal Antibody (Cat# AAA9604994)

Full Name
CADM3 Antibody
Gene Names
CADM3; BIgR; NECL1; TSLL1; IGSF4B; Necl-1; synCAM3
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TRPV4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

TRPV4, Polyclonal Antibody (Cat# AAA9125407)

Full Name
TRPV4 Polyclonal Antibody
Gene Names
TRPV4; SMAL; VRL2; CMT2C; SPSMA; TRP12; VROAC; HMSN2C; OTRPC4; SSQTL1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Activity Data (Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. NGF is also involved in pathways besides those regulating the life cycle of neurons. a nerve growth factor (NGF)-dependent TF-1 subclone cell line suitable for determination of bioactivity of recombinant NGF(rhNGF), then TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 contain various concentrations of recombinant rat NGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of TF-1 cells after incubation with TF-1 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 assay after incubation with recombinant NGF for 72h. The result was shown in Figure 2. It was obvious that NGF significantly increased cell viability of TF-1 cells.(A) TF-1 cells cultured in 1640, stimulated with 0.1ng/mL NGF for 72h; (B) Unstimulated TF-1 cells cultured in 1640 for 72h.Figure. Cell proliferation of TF-1 cells after stimulated with NGF.)

Nerve Growth Factor (NGF), Active Protein (Cat# AAA2105076)

Full Name
Active Nerve Growth Factor (NGF)
Gene Names
Ngf; Ngfb; beta-NGF
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$150/0.01 mg | $255/0.05 mg | $385/0.1 mg | $470/0.2 mg | $895/0.5 mg | $1,315/1 mg | $3,210/5 mg | $14,445/5x5 mg
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Mouse Recombinant Monoclonal Antibody (rFOXA1/1515).)

FOXA1/HNF3A, Monoclonal Antibody (Cat# AAA4380019)

Full Name
FOXA1/HNF3A
Gene Names
FOXA1; HNF3A; TCF3A
Reactivity
Human, Rat. Others not known.
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunofluorescence (IF) (MBS9605365 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

BORG2, Polyclonal Antibody (Cat# AAA9605365)

Full Name
BORG2 Antibody
Gene Names
CDC42EP3; UB1; CEP3; BORG2
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9601556 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

P62/SQSTM1, Polyclonal Antibody (Cat# AAA9601556)

Full Name
P62/SQSTM1 Antibody
Gene Names
SQSTM1; p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA, IHC
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9607093 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IGF2BP1, Polyclonal Antibody (Cat# AAA9607093)

Full Name
IGF2BP1 Antibody
Gene Names
IGF2BP1; IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TIPARP antibody (MBS9134334) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

TIPARP, Polyclonal Antibody (Cat# AAA9134334)

Full Name
TIPARP Polyclonal Antibody
Gene Names
TIPARP; PARP7; ARTD14; pART14
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using FSHR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.)

FSHR, Polyclonal Antibody (Cat# AAA128093)

Full Name
FSHR Polyclonal Antibody
Gene Names
FSHR; LGR1; ODG1; FSHRO
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ACE antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

ACE1, Polyclonal Antibody (Cat# AAA126472)

Full Name
ACE1
Gene Names
ACE; DCP; ICH; ACE1; DCP1; CD143; MVCD3
Reactivity
Human, Mouse
Applications
WB
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using EDAR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

EDAR, Polyclonal Antibody (Cat# AAA129455)

Full Name
EDAR Polyclonal Antibody
Gene Names
EDAR; DL; ED3; ED5; ED1R; EDA3; HRM1; EDA1R; ECTD10A; ECTD10B; EDA-A1R
Reactivity
Human
Applications
WB, IHC, FC/FACS
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunohistochemistry (IHC) (Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

SLC22A5/OCTN2, Polyclonal Antibody (Cat# AAA2401502)

Full Name
Anti-SLC22A5/OCTN2 Antibody
Gene Names
SLC22A5; CDSP; OCTN2
Reactivity
Human
Predicted: Mouse, Rat
Applications
IF, IHC, IHC, WB
Purity
Affinity Purified
Pricing
$610/0.05 mL | $2,685/5x0.05 mL
Immunohistochemistry (IHC) (MBS9609445 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

RPL24, Polyclonal Antibody (Cat# AAA9609445)

Full Name
RPL24 Antibody
Gene Names
RPL24; L24; HEL-S-310
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL

CD106 / VCAM1, Monoclonal Antibody (Cat# AAA439086)

Full Name
CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody
Gene Names
VCAM1; CD106; INCAM-100
Reactivity
Human
Applications
FC/FACS, IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Flow Cytometry (FC/FACS) (Flow Cytometry of HT29 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Ep-CAM Monoclonal Antibody (SPM134).)

Ep-CAM /CD326, Monoclonal Antibody (Cat# AAA439785)

Full Name
Ep-CAM /CD326 (Epithelial Marker) Mouse Monoclonal Antibody
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human.
Does not react with Rat
Applications
FC/FACS, IF
Purity
Purified Ab conjugated to PE
Pricing
$390/100 Tests | $1,710/5x100 Tests
Activity Data (Regenerating Islet Derived Protein 3 Beta (REG3b) also known as PAP-I and HIP is bactericidal C-type lectin which acts against several intestinal Gram-positive bacteria and Gram-negative bacteria. The Reg family proteins have been implicated in a range of physiological processes including acting as acute phase reactants, lectins, survival/growth factors for insulin-producing pancreatic beta-cells, neural cells, and epithelial cells of the digestive system. To test the effect of REG3b on cell proliferation of SK-N-SH, SK-N-SH cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of REG3b. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C .Cell proliferation of SK-N-SH cells after incubation with REG3b for 72h observed by inverted microscope was shown in Figure 1.)

Regenerating Islet Derived Protein 3 Beta (REG3b), Active Protein (Cat# AAA2097321)

Full Name
Active Regenerating Islet Derived Protein 3 Beta (REG3b)
Gene Names
Reg3b; HIP; Pap; PAP1; REG-III
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$235/0.01 mg | $510/0.05 mg | $790/0.1 mg | $980/0.2 mg | $1,915/0.5 mg | $2,830/1 mg | $6,925/5 mg
Immunofluorescence (IF) (MBS9605273 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

DDX19B, Polyclonal Antibody (Cat# AAA9605273)

Full Name
DDX19B Antibody
Gene Names
DDX19B; DBP5; RNAh; DDX19
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of Tyrosine Hydroxylase phosphorylation expression in UV treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Tyrosine Hydroxylase, Polyclonal Antibody (Cat# AAA9600885)

Full Name
Phospho-Tyrosine Hydroxylase (Ser31) Antibody
Gene Names
TH; TYH; DYT14; DYT5b
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9601262 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

CaMK2, Polyclonal Antibody (Cat# AAA9601262)

Full Name
Phospho-CaMK2 (Thr286) Antibody
Gene Names
CAMK2A; CAMKA; MRD53; MRT63; CaMKIINalpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using Phospho-PDK1-S241 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

PDK1-S241, Antibody (Cat# AAA9125436)

Full Name
Phospho-PDK1-S241
Gene Names
PDPK1; PDK1; PDPK2; PDPK2P; PRO0461
Reactivity
Human
Applications
WB
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using GEM antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

GEM, Polyclonal Antibody (Cat# AAA9125260)

Full Name
GEM
Gene Names
GEM; KIR
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using PENK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 20s.)

PENK, Polyclonal Antibody (Cat# AAA9125144)

Full Name
PENK Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using REG4 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

REG4, Polyclonal Antibody (Cat# AAA9133177)

Full Name
REG4 Polyclonal Antibody
Gene Names
REG4; GISP; RELP; REG-IV
Reactivity
Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL

CD74, Monoclonal Antibody (Cat# AAA438349)

Full Name
CD74
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human.
Applications
EIA, FC/FACS, IF, WB, IP, IH
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Western blot analysis of Phospho-RhoA (Ser188) expression in various lysates)

RhoA, Polyclonal Antibody (Cat# AAA9601121)

Full Name
Phospho-RhoA (Ser188) Antibody
Gene Names
RHOA; ARHA; ARH12; RHO12; RHOH12
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, EIA
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NLRC4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

NLRC4, Polyclonal Antibody (Cat# AAA9126899)

Full Name
NLRC4
Gene Names
NLRC4; CLAN; IPAF; AIFEC; CLAN1; CLANA; CLANB; CLANC; CLAND; FCAS4; CARD12; CLR2.1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Activity Data (Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. It was reported that FNDC5 significantly decreased cell number, migration and viability through apoptosis in malignant MDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of FNDC5 for 48h, then MDA-MB-231 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC.Cell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observed by inverted microscope was shown in Figure 1.)

Fibronectin Type III Domain Containing Protein 5 (FNDC5), Active Protein (Cat# AAA2086089)

Full Name
Active Fibronectin Type III Domain Containing Protein 5 (FNDC5)
Gene Names
FNDC5; FRCP2; irisin
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $650/0.2 mg | $1,260/0.5 mg | $1,870/1 mg | $4,595/5 mg
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using EIF2AK1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

EIF2AK1, Polyclonal Antibody (Cat# AAA128920)

Full Name
EIF2AK1 Polyclonal Antibody
Gene Names
EIF2AK1; HCR; HRI
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CTLA4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 5s.)

CTLA4, Polyclonal Antibody (Cat# AAA126302)

Full Name
CTLA4 Polyclonal Antibody
Gene Names
CTLA4; CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
Reactivity
Human
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with pan Cytokeratin Monoclonal Antibody cocktail (SPM115 + SPM116).)

Cytokeratin, pan, Monoclonal Antibody (Cat# AAA438464)

Full Name
Cytokeratin, pan (Epithelial Marker) Mouse Monoclonal Antibody
Gene Names
KRT77; K1B; KRT1B
Reactivity
Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunofluorescence (IF) (MBS9600594 staining MCF-7 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

Histone H3, Polyclonal Antibody (Cat# AAA9600594)

Full Name
Histone H3 Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$190/0.05 mL | $245/0.1 mL | $305/0.2 mL | $855/1 mL | $3,695/5x1 mL

CD33, Monoclonal Antibody (Cat# AAA438512)

Full Name
CD33
Gene Names
CD33; p67; SIGLEC3; SIGLEC-3
Reactivity
Human.
Applications
FC/FACS
Purity
Purified Ab conjugated to PE
Pricing
$390/0.5 mL | $1,710/5x0.5 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using SAA3 Rabbit pAb (MBS9131885) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ( MBS128200 ) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5min.)

SAA3P, Polyclonal Antibody (Cat# AAA9131885)

Full Name
SAA3P Polyclonal Antibody
Gene Names
Saa3; l7R3; Saa-3; AV098916
Applications
WB
Purity
Affinity Purification
Pricing
$225/0.05 mL | $305/0.1 mL | $465/0.2 mL

CD11/CD18, Monoclonal Antibody (Cat# AAA375382)

Full Name
Mouse anti CD11/CD18 [24]: Fluorescein
Reactivity
Human
Applications
FC, ICC, IF
Purity
Purified on protein A from tissue culture supernatant.
Pricing
$500/0.05 mL | $2,110/5x0.05 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CLEC1B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

CLEC1B, Polyclonal Antibody (Cat# AAA9129243)

Full Name
CLEC1B Polyclonal Antibody
Gene Names
CLEC1B; CLEC2; CLEC2B; PRO1384; QDED721; 1810061I13Rik
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TONSL antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

TONSL, Polyclonal Antibody (Cat# AAA9133084)

Full Name
TONSL Polyclonal Antibody
Gene Names
TONSL; IKBR; NFKBIL2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
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