Mouse anti-Human CD106 / VCAM1 Monoclonal Antibody | anti-VCAM1 antibody

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

Cat. Num. AAA439086

• Gene Names: VCAM1; CD106; INCAM-100
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD106 / VCAM1; Monoclonal Antibody; CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody; CD106; INCAM-100; L1CAM; Vascular Cell Adhesion Molecule 1 (VCAM1); Vascular cell adhesion protein 1; anti-VCAM1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: B-K9
• Specificity: Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This MAb inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 739

• Applicable Applications for anti-VCAM1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (1-2ug/ml)
• Cellular Localization: Cell Surface
• Immunogen: Activated human umbilical vein endothelial cells (HUVEC)
• Hu-Chromosome Location: 1q21.2
• Positive Control: Human placenta or tonsil
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Schlossman SF et al. eds. Leukocyte Typing V, p1764-1767, Oxford University Press, Oxford, 1995. Vermot-Desroches C et al. Heterogeneity of antigen expression among human umbilical cord vascular endothelial cells: identification of cell subsets by co-expression of haemopoietic antigens. Immunol Lett 1995, 48(1):1-9 Rice GE et al. Vascular and nonvascular expression of INCAM-110. A target for mononuclear leukocyte adhesion in normal and inflamed human tissues. Am J Pathol 1991, 138(2):385-393 Huang MJ et al. Expression of vascular cell adhesion molecule-1 by follicular dendritic cells. Leuk Lymphoma 1995, 18(3-4):259-264. Rosen GD et al. Roles for the integrin VLA-4 and its counter receptor VCAM-1 in myogenesis. Cell 1992, 69(7):1107-1119 Bevilacqua MP. Endothelial-leukocyte adhesion molecules. Annu Rev Immunol 1993;11:767-804 Foster CA. VCAM-1/alpha 4-integrin adhesion pathway: therapeutic target for allergic inflammatory disorders. J Allergy Clin Immunol 1996, 98(6 Pt 2):S270-277

NCBI and Uniprot Product Information

• NCBI GI #: 4507875
• NCBI GeneID: 7412
• NCBI Accession #: NP_001069.1
• NCBI GenBank Nucleotide #: NM_001078.3
• UniProt Accession #: P19320; Q6NUP8; A8K6R7; B4DKS4; E9PDD1
• Molecular Weight: 110kDa
• NCBI Official Full Name: vascular cell adhesion protein 1 isoform a
• NCBI Official Synonym Full Names: vascular cell adhesion molecule 1
• NCBI Official Symbol: VCAM1
• NCBI Official Synonym Symbols: CD106; INCAM-100
• NCBI Protein Information: vascular cell adhesion protein 1
• UniProt Protein Name: Vascular cell adhesion protein 1
• Protein Family: Vascular cell adhesion protein
• UniProt Gene Name: VCAM1
• UniProt Synonym Gene Names: L1CAM; V-CAM 1; VCAM-1
• UniProt Entry Name: VCAM1_HUMAN

NCBI Description

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: apical part of cell; cell surface; early endosome; endoplasmic reticulum; external side of plasma membrane; extracellular space; filopodium; Golgi apparatus; microvillus; plasma membrane; podosome

Molecular Function: amine oxidase activity; cell adhesion molecule binding; integrin binding

Biological Process: amine metabolic process; B cell differentiation; cell adhesion; cell-matrix adhesion; extracellular matrix organization and biogenesis; heterophilic cell adhesion; leukocyte adhesion; leukocyte tethering or rolling; membrane to membrane docking; positive regulation of T cell proliferation; regulation of immune response