Mouse anti-Human. CD74 Monoclonal Antibody | anti-CD74 antibody

CD74

Cat. Num. AAA438349

• Gene Names: CD74; II; DHLAG; HLADG; Ia-GAMMA
• Reactivity: Human.
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry
• Synonyms: CD74; Monoclonal Antibody; CLIP; DHLAG; Gamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; HLADR-gamma (HLADG); Ia antigen-associated invariant chain; la-gamma; Major histocompatibility complex class II invariant chain; MHC HLA-DR gamma chain; anti-CD74 antibody

• Host: Mouse
• Reactivity: Human.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: BU45
• Specificity: Recognizes proteins of 33, 35 and 41kDa, which are identified as various isoforms of CD74. Its epitope is localized in the extracellular domain of CD74. CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinomas. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-CD74 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Immunohistology (IH)
• Application Notes: ELISA (For coating, order Ab without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Western Blotting (0.5-1ug/ml); Immunoprecipitation (1-2ug/500ug protein lysate); Immunohistology (Frozen) (0.5-1ug/ml for 30 minutes at RT) Optimal dilution for a specific application should be determined.
• Localization: Cell surface and paranuclear globular
• Positive Control: Daudi Cells. Tonsil or lymph node.
• Human Chromosomal Location: 5q33.1
• Immunogen: B lymphoblastoid cell line HFB1
• Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide
• Preparation and Storage: Store at 2 to 8 degree C. Antibody is stable for 24 months.

References

1. Wraight, C.J. et al. (1990) Human major histocompatibility complex class II invariant chain is expressed on the cell surface. J Biol Chem.265:5787.

NCBI and Uniprot Product Information

• NCBI GeneID: 972
• UniProt Accession #: P044233; P04233
• Molecular Weight: 33-41kDa
• NCBI Official Synonym Full Names: CD74 molecule, major histocompatibility complex, class II invariant chain
• NCBI Official Symbol: CD74
• NCBI Official Synonym Symbols: II; DHLAG; HLADG; Ia-GAMMA
• NCBI Protein Information: HLA class II histocompatibility antigen gamma chain; p33; HLA-DR-gamma; MHC HLA-DR gamma chain; Ia-associated invariant chain; gamma chain of class II antigens; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
• Protein Family: H-2 class II histocompatibility antigen

NCBI Description

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]