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Type
Reactivity
Gene Name
Isotype
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Application
Clone
10000 results for "Antibodies to Human Proteins" - showing 650-700
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808819)
Full Name
Methylglyoxal Antibody, Clone 9F11: Biotin
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,255/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Hexanoyl-Lysine adduct, Monoclonal Antibody (Cat# AAA808659)
Full Name
Hexanoyl-Lysine adduct Antibody, Clone 5D9: HRP
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$545/0.1 mg | $2,215/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
4-Hydroxy-2-hexenal, Monoclonal Antibody (Cat# AAA808683)
Full Name
4-Hydroxy-2-hexenal Antibody, Clone 6F10: ATTO 390
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA, FC/FACS
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,280/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808817)
Full Name
Methylglyoxal Antibody, Clone 9F11: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36. Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 um stacks.. Courtesy of: Francesco Paonessa.)
Tau, Monoclonal Antibody (Cat# AAA8005566)
Full Name
Tau Antibody (pSer202/ pThr205), Clone AH36: ATTO 390
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human
Applications
WB, DB, ICC, IF, EIA
Purity
Affinity Purified
Pricing
$625/0.1 mg | $2,585/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
VPS35, Monoclonal Antibody (Cat# AAA8005625)
Full Name
VPS35 Antibody, Clone 5A9: Biotin
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IP
Purity
Protein G Purified
Pricing
$535/0.1 mg | $2,170/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CORO2A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
CORO2A, Polyclonal Antibody (Cat# AAA9134472)
Full Name
CORO2A Polyclonal Antibody
Gene Names
CORO2A; IR10; WDR2; CLIPINB
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using HLA-DOA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90S.)
HLA-DOA, Polyclonal Antibody (Cat# AAA9135263)
Full Name
HLA-DOA Polyclonal Antibody
Gene Names
HLA-DOA; HLADZ; HLA-DNA; HLA-DZA
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of U-937 cells, using Phospho-PRKCD-T505 antibody at 1:2000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 60s.)
PRKCD-T505, Polyclonal Antibody (Cat# AAA9135629)
Full Name
Phospho-PRKCD-T505 pAb
Gene Names
PRKCD; MAY1; PKCD; ALPS3; CVID9; nPKC-delta
Reactivity
Human
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of APG8b (MAP1LC3B) Antibody (T93/Y99) in MCF-7 cell line lysates (35ug/lane). MAP1LC3B (arrow) was detected using the purified Pab.)
APG8b (MAP1LC3B)-T93/Y99, Polyclonal Antibody (Cat# AAA9211055)
Full Name
APG8b (MAP1LC3B)-T93/Y99 Antibody (Center)
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, mouse
Applications
FC/FACS, EIA, WB, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(All lanes : Anti-SNRPD3 Antibody (C-term) at 1:1000 dilutionLane 1: MCF-7 whole cell lysateLane 2: HL-60 whole cell lysateLane 3: Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
SNRPD3, Polyclonal Antibody (Cat# AAA9232107)
Full Name
SNRPD3 Antibody (C-term)
Gene Names
SNRPD3; SMD3; Sm-D3
Reactivity
Human
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of A431 cells, using Phospho-VASP-S157 antibody at 1:2000 dilution. A431 cells were treated by Forskolin (10uM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
VASP-S157, Polyclonal Antibody (Cat# AAA9135617)
Full Name
Phospho-VASP-S157 pAb
Reactivity
Human, Rat
Applications
WB, IP
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(APG8b(MAP1LC3B) Antibody (T29) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the APG8b(MAP1LC3B) antibody detected the APG8b(MAP1LC3B) protein (arrow).)
APG8b (MAP1LC3B), Polyclonal Antibody (Cat# AAA9201154)
Full Name
APG8b (MAP1LC3B) Antibody (N-term T29)
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat)
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunoprecipitation (IP)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 11H10 depletes VPS35 from the A549 cell extract..)
VPS35, Monoclonal Antibody (Cat# AAA8005703)
Full Name
VPS35 Antibody, Clone 11H10: FITC
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, IP
Purity
Protein G Purified
Pricing
$530/0.1 mg | $2,155/5x0.1 mg
Immunoprecipitation (IP)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 11H10 depletes VPS35 from the A549 cell extract..)
VPS35, Monoclonal Antibody (Cat# AAA8005704)
Full Name
VPS35 Antibody, Clone 11H10: HRP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, IP
Purity
Protein G Purified
Pricing
$520/0.1 mg | $2,135/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808791)
Full Name
Methylglyoxal Antibody, Clone 9E7: ATTO 390
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,280/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
VPS35, Monoclonal Antibody (Cat# AAA8005655)
Full Name
VPS35 Antibody, Clone 8A3: FITC
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IP
Purity
Protein G Purified
Pricing
$530/0.1 mg | $2,155/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CALCB antibody (MBS9128519) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
CALCB, Polyclonal Antibody (Cat# AAA9128519)
Full Name
CALCB Polyclonal Antibody
Gene Names
CALCB; CALC2; CGRP2; CGRP-II
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using Phospho-NR3C1-S211 antibody at 1:2000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 10s.)
NR3C1-S211, Polyclonal Antibody (Cat# AAA9135614)
Full Name
Phospho-NR3C1-S211 pAb
Gene Names
NR3C1; GR; GCR; GRL; GCCR; GCRST
Reactivity
Human
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
APRT, Polyclonal Antibody (Cat# AAA9232055)
Full Name
APRT Antibody (N-term)
Gene Names
APRT; AMP; APRTD
Reactivity
Human
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Dityrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Dityrosine, Monoclonal Antibody (Cat# AAA808882)
Full Name
Dityrosine Antibody, Clone 10A6: ATTO 488
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,275/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Tau Monoclonal Antibody, Clone AH36. Tissue: iPSC-derived cortical excitatory neurons. Species: Human. Primary Antibody: Rabbit Anti-Tau Monoclonal Antibody at 1:500 for Overnight. Secondary Antibody: Donkey anti-rabbit: Alexa Fluor 488 at 1:1000. Counterstain: DAPI. A) iPSC-derived neurons from non-demented control (NDC). B) iPSC-derived neurons from subject with P301L MAPT mutation. Images acquired using an automated Opera Phoenix system. Each field of view is a max projection from 10 planes of 1 um stacks.. Courtesy of: Francesco Paonessa.)
Tau, Monoclonal Antibody (Cat# AAA8005583)
Full Name
Tau Antibody (pSer202/ pThr205), Clone AH36: FITC
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human
Applications
WB, DB, ICC, IF, EIA
Purity
Affinity Purified
Pricing
$615/0.1 mg | $2,540/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
VPS35, Monoclonal Antibody (Cat# AAA8005614)
Full Name
VPS35 Antibody, Clone 5A9: ATTO 390
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IP
Purity
Protein G Purified
Pricing
$540/0.1 mg | $2,185/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with AP22330a at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).)
MKS1, Polyclonal Antibody (Cat# AAA9231277)
Full Name
MKS1 Antibody (N-Term)
Gene Names
MKS1; MES; MKS; BBS13; POC12; JBTS28
Reactivity
Human
Applications
IF, FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
VPS35, Monoclonal Antibody (Cat# AAA8005639)
Full Name
VPS35 Antibody, Clone 8A3: ATTO 488
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IP
Purity
Protein G Purified
Pricing
$535/0.1 mg | $2,180/5x0.1 mg
Western Blot (WB)
(All lanes : Anti-PLOD1 Antibody (N-term) at 1:2000 dilutionLane 1: A431 whole cell lysatesLane 2: MCF-7 whole cell lysatesLane 3: U-2OS whole cell lysatesLane 4: U-87 MG whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
PLOD1, Polyclonal Antibody (Cat# AAA9215038)
Full Name
PLOD1 Antibody (N-term)
Gene Names
PLOD1; LH; LH1; LLH; EDS6; PLOD
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Dityrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Dityrosine, Monoclonal Antibody (Cat# AAA808880)
Full Name
Dityrosine Antibody, Clone 10A6
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$505/0.1 mg | $2,050/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using S1PR1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
S1PR1, Polyclonal Antibody (Cat# AAA9132715)
Full Name
S1PR1 Polyclonal Antibody
Gene Names
S1PR1; EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of RCE1 (arrow) using rabbit polyclonal RCE1 Antibody (W57). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCE1 gene.)
RCE1, Polyclonal Antibody (Cat# AAA9200807)
Full Name
RCE1 Antibody (N-term W57)
Gene Names
RCE1; FACE2; RCE1A; RCE1B
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using Phospho-TBC1D4-S588 antibody at 1:2000 dilution. HeLa cells were treated by IGF-1 (50ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 30s.)
TBC1D4-S588, Polyclonal Antibody (Cat# AAA9135643)
Full Name
Phospho-TBC1D4-S588 pAb
Gene Names
TBC1D4; AS160; NIDDM5
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(All lanes : Anti-CBFB Antibody (Center) at 1:1000 dilutionLane 1: K562 whole cell lysatesLane 2: Jurkat whole cell lysatesLane 3: Raji whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
CBFB, Polyclonal Antibody (Cat# AAA9232041)
Full Name
CBFB Antibody (Center)
Gene Names
CBFB; PEBP2B
Reactivity
Human
Predicted: Mouse
Predicted: Mouse
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
VPS35, Monoclonal Antibody (Cat# AAA8005661)
Full Name
VPS35 Antibody, Clone 10A8
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IP
Purity
Protein G Purified
Pricing
$485/0.1 mg | $1,950/5x0.1 mg
Immunoprecipitation (IP)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 11H10 depletes VPS35 from the A549 cell extract..)
VPS35, Monoclonal Antibody (Cat# AAA8005706)
Full Name
VPS35 Antibody, Clone 11H10: PerCP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, IP
Purity
Protein G Purified
Pricing
$540/0.1 mg | $2,185/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808810)
Full Name
Methylglyoxal Antibody, Clone 9F11: ATTO 488
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,275/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Hexanoyl-Lysine adduct, Monoclonal Antibody (Cat# AAA808661)
Full Name
Hexanoyl-Lysine adduct Antibody, Clone 5D9: PerCP
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,270/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Dityrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Dityrosine, Monoclonal Antibody (Cat# AAA808884)
Full Name
Dityrosine Antibody, Clone 10A6: ATTO 594
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,275/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Acrolein, Monoclonal Antibody (Cat# AAA808583)
Full Name
Acrolein Antibody, Clone 2H2: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of HT-29 cells, using Phospho-TP53-S33 antibody at 1:2000 dilution. HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
TP53-S33, Polyclonal Antibody (Cat# AAA9135616)
Full Name
Phospho-TP53-S33 pAb
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
WB, IP
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Immunohistochemistry (IHC)-Paraffin
(ATP5H antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP5H antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
ATP5H, Polyclonal Antibody (Cat# AAA9232086)
Full Name
ATP5H Antibody (Center)
Gene Names
ATP5PD; ATPQ; APT5H; ATP5H
Reactivity
Human
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(All lanes : Anti-PYCR1 Antibody (C-term) at 1:1000 dilutionLane 1: HepG2 whole cell lysateLane 2: K562 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
PYCR1, Polyclonal Antibody (Cat# AAA9232094)
Full Name
PYCR1 Antibody (C-term)
Gene Names
PYCR1; P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B
Reactivity
Human
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(All lanes : Anti-NOVA1 Antibody (Center)(MBS9211519) at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 49 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NOVA1, Polyclonal Antibody (Cat# AAA9211519)
Full Name
NOVA1 Antibody (Center)
Gene Names
NOVA1; Nova-1
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
WB, EIA, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: mouse liver) using a 10 % SDS PAGE gel and PHLP antibody at a dilution of 1:2000)
PHLP, Polyclonal Antibody (Cat# AAA835693)
Full Name
PHLP antibody
Gene Names
PDCL; PhLP
Applications
IF, ICC, WB
Purity
PHLP antibody was purified by antigen-affinity chromatography
Pricing
$700/0.1 mL | $3,130/5x0.1 mL
Western Blot (WB)
(Anti-SIGLEC11 at 1:1000 dilution + HepG2 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 76 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
SIGLEC11, Polyclonal Antibody (Cat# AAA9217090)
Full Name
SIGLEC11 Antibody (N-term)
Reactivity
Human
Applications
WB, IHC, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
VPS35, Monoclonal Antibody (Cat# AAA8005670)
Full Name
VPS35 Antibody, Clone 10A8: Alkaline Phosphatase
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IP
Purity
Protein G Purified
Pricing
$535/0.1 mg | $2,165/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
VPS35, Monoclonal Antibody (Cat# AAA8005613)
Full Name
VPS35 Antibody, Clone 5A9
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IP
Purity
Protein G Purified
Pricing
$485/0.1 mg | $1,950/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808809)
Full Name
Methylglyoxal Antibody, Clone 9F11: ATTO 390
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,280/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Acrolein, Monoclonal Antibody (Cat# AAA808590)
Full Name
Acrolein Antibody, Clone 2H2: RPE
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,260/5x0.1 mg
Immunofluorescent (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma cell line) cells labeling HINT1 with MBS9200257 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue))
HINT1, Monoclonal Antibody (Cat# AAA9200257)
Full Name
HINT1 Antibody
Gene Names
HINT1; HINT; NMAN; PKCI-1; PRKCNH1
Reactivity
Human, mouse, rat
Applications
F, IHC-P, FC, WB,E
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of Raji cells, using Phospho-PLCG2-Y1217 antibody at 1:2000 dilution. Raji cells were treated by Pervanadate (1mM) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
PLCG2-Y1217, Polyclonal Antibody (Cat# AAA9135658)
Full Name
Phospho-PLCG2-Y1217 pAb
Gene Names
PLCG2; FCAS3; APLAID; PLC-IV; PLC-gamma-2
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of K562 cells, using Phospho-CRK-Y221 antibody at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 degree C for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 10s.)
CRK-Y221, Polyclonal Antibody (Cat# AAA9135675)
Full Name
Phospho-CRK-Y221 pAb
Gene Names
CRK; p38; CRKII
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
