Western Blot (WB)
(Western blot analysis of APG8b (MAP1LC3B) Antibody (T93/Y99) in MCF-7 cell line lysates (35ug/lane). MAP1LC3B (arrow) was detected using the purified Pab.)
Rabbit anti-Human, mouse APG8b (MAP1LC3B)-T93/Y99 Polyclonal Antibody | anti-MAP1LC3B antibody
APG8b (MAP1LC3B)-T93/Y99 Antibody (Center)
Western Blot (WB)
(Western blot analysis of APG8b (MAP1LC3B) Antibody (T93/Y99) in MCF-7 cell line lysates (35ug/lane). MAP1LC3B (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of APG8b (MAP1LC3B) Antibody (T93/Y99) in MCF-7 cell line lysates (35ug/lane). MAP1LC3B (arrow) was detected using the purified Pab.)
Western Blot (WB)
(APG8b (MAP1LC3B)-T93/Y99 western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the MAP1LC3B antibody detected MAP1LC3B protein (arrow).)
Western Blot (WB)
(APG8b (MAP1LC3B)-T93/Y99 western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the MAP1LC3B antibody detected MAP1LC3B protein (arrow).)
Immunohistochemistry (IHC)
(APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
microtubule-associated MAP1A and MAP1B proteins, which are involved
in microtubule assembly and important for neurogenesis. Studies on
the rat homolog implicate a role for this gene in autophagy, a
process that involves the bulk degradation of cytoplasmic
component.
Othman, E.Q., et al. J. Clin. Lab. Anal. 23(4):249-258(2009)
Komatsu, M., et al. Cell 131(6):1149-1163(2007)
Stelzl, U., et al. Cell 122(6):957-968(2005)
Tanida, I., et al. J. Biol. Chem. 279(46):47704-47710(2004)
He, H., et al. J. Biol. Chem. 278(31):29278-29287(2003)
Tanida, I., et al. J. Biol. Chem. 277(16):13739-13744(2002)
Tanida, I., et al. Biochem. Biophys. Res. Commun. 292(1):256-262(2002)
Kabeya, Y., et al. EMBO J. 19(21):5720-5728(2000)
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
NCBI and Uniprot Product Information
NCBI Description
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
Uniprot Description
LC3B: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Recruits proteins such as SQSTM1 and NBR1 to the autophagosome.
Protein type: Autophagy; Ubiquitin-like modifier; Vesicle; Microtubule-binding
Chromosomal Location of Human Ortholog: 16q24.2
Cellular Component: microtubule; extrinsic to membrane; mitochondrion; endomembrane system; autophagic vacuole; intracellular; cytoplasmic vesicle; axoneme; cytosol
Molecular Function: protein binding; microtubule binding; GABA receptor binding
Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation
Research Articles on MAP1LC3B
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Product Notes
The MAP1LC3B map1lc3b (Catalog #AAA9211055) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 74-106. The APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's APG8b (MAP1LC3B)-T93/Y99 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the MAP1LC3B map1lc3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APG8b (MAP1LC3B)-T93/Y99, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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