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8524 results for " TISSUES AND CELLS" - showing 6900-6950


Western Blot (WB) (Western blot analysis of SIRT2 expression in SKBR-3 cell lysate (MBS179323).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SIRT2 )

SIRT2/Sirtuin 2, Monoclonal Antibody (Cat# AAA179323)

Full Name
Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody
Gene Names
SIRT2; SIR2; SIR2L; SIR2L2
Reactivity
Human, Rat
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunofluorescence (IF) (Confocal immunofluorescence analysis of Hela cells using INHA mouse mAb (green). Red)

INHA, Monoclonal Antibody (Cat# AAA475721)

Full Name
Anti-INHA (Inhibin alpha) Mouse mAb
Reactivity
Human
Applications
IHC, ICC
Pricing
$440/0.1 mL | $1,530/5x0.1 mL

S1PR2 / Sphingosine 1-Phosphate Receptor 2, Recombinant Protein (Cat# AAA2901743)

Full Name
Human S1PR2 / Sphingosine 1-Phosphate Receptor 2 Recombinant Protein
Gene Names
S1PR2; EDG5; H218; LPB2; S1P2; AGR16; EDG-5; DFNB68; Gpcr13
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
Western Blot (WB) (Western blot analysis of RUNX1/RUNX2/RUNX3 expression in MOLT4 cell lysate (MBS179090).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RUNX1 )

RUNX1/RUNX2/RUNX3, Monoclonal Antibody (Cat# AAA179090)

Full Name
Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of SIRT2 expression in SH-SY5Y cell lysate (MBS179322).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SIRT2 )

SIRT2/Sirtuin 2, Monoclonal Antibody (Cat# AAA179322)

Full Name
Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody
Gene Names
SIRT2; SIR2; SIR2L; SIR2L2
Reactivity
Human, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of MUC1 (Phospho-Ser1227) using 293 whole cell lysates.-/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide).)

CD227/MUC1, Polyclonal Antibody (Cat# AAA9614290)

Full Name
Phospho-CD227/MUC1 (Ser1227) Antibody
Gene Names
MUC1; EMA; PEM; PUM; KL-6; MAM6; PEMT; CD227; H23AG; MUC-1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from Mouse brain, using Phospho-Dnmt1 (Ser714) Antibody. The lane on the left was treated with blocking peptide.)

Dnmt1, Polyclonal Antibody (Cat# AAA9612760)

Full Name
Phospho-Dnmt1 (Ser714) Antibody
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
Peptide affinity purification
Pricing
$310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from COLO cells, using ARHGEF3 antibody.)

ARHGEF3, Antibody (Cat# AAA8515138)

Full Name
ARHGEF3 Antibody
Gene Names
ARHGEF3; GEF3; STA3; XPLN
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)

RELT / TNFRSF19L, Recombinant Protein (Cat# AAA2546198)

Full Name
Recombinant Human RELT / TNFRSF19L Protein (His & Fc tag)
Gene Names
RELT; TRLT; TNFRSF19L
Purity
> 90 % as determined by SDS-PAGE
Pricing
$440/0.05 mg | $675/0.1 mg | $3,030/5x0.1 mg
Western Blot (WB) (Western blot analysis using NPC1 mAb against human NPC1 recombinant protein. (Expected MW is 37.6 kDa) (Left). Western blot analysis using NPC1 mAb against HEK293 (1) and NPC1 (AA:34-174)-hIgGFc transfected HEK293 (2) cell lysate (Right).)

NPC1, Monoclonal Antibody (Cat# AAA8527785)

Full Name
Mouse Monoclonal Antibody to NPC1
Gene Names
Npc1; spm; lcsd; C85354; nmf164; D18Ertd139e; D18Ertd723e; A430089E03Rik
Reactivity
Human
Applications
WB, IHC, FC/FACS
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)

INHA, Blocking Peptide (Cat# AAA9226289)

Full Name
INHA Antibody (N-term) Blocking Peptide
Western Blot (WB) (Anti-KIAA1524 Antibody (Center) at 1:2000 dilution + Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 102 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

KIAA1524, Polyclonal Antibody (Cat# AAA9231250)

Full Name
KIAA1524 Antibody (Center)
Gene Names
CIP2A; p90; KIAA1524
Reactivity
Human
Applications
WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Flow Cytometry (FC/FACS) (Overlay histogram showing Hela cells stained with AP22343b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22343b, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

KCNH1, Polyclonal Antibody (Cat# AAA9231291)

Full Name
KCNH1 Antibody (C-Term)
Gene Names
KCNH1; EAG; EAG1; ZLS1; TMBTS; h-eag; hEAG1; Kv10.1
Reactivity
Human
Predicted: Human
Applications
FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL

CD173, Monoclonal Antibody (Cat# AAA6253453)

Full Name
CD173 (MaxLight 490)
Reactivity
Human, Porcine
Applications
FC/FACS
Purity
Purified
Pricing
$865/0.1 mL | $3,885/5x0.1 mL

MIIP, Blocking Peptide (Cat# AAA9225496)

Full Name
MIIP Antibody (C-term) Blocking Peptide
Gene Names
MIIP; IIP45

Heart And Neural Crest Derivatives Expressed Protein 1 (HAND1), Polyclonal Antibody (Cat# AAA2066386)

Full Name
FITC-Linked Polyclonal Antibody to Heart And Neural Crest Derivatives Expressed Protein 1 (HAND1)
Gene Names
HAND1; Hxt; eHand; Thing1; bHLHa27
Reactivity
Mouse
Applications
WB, IHC, ICC, IF
Purity
Antigen-specific affinity chromatography
Pricing
$210/0.1 mL | $265/0.2 mL | $480/0.5 mL | $590/1 mL | $1,655/5x1 mL

Ptk7, Blocking Peptide (Cat# AAA9229818)

Full Name
Mouse Ptk7 Blocking Peptide (C-term)
Gene Names
Ptk7; chz; mPTK7/CCK4; 8430404F20Rik
Immunofluorescence (IF)

NCAM / CD56, Antibody (Cat# AAA570443)

Full Name
Mouse anti NCAM / CD56
Reactivity
Human
Applications
FC/FACS, ICC, IHC, WB, EIA
Pricing
$475/0.1 mg | $1,995/5x0.1 mg

CFL1, Blocking Peptide (Cat# AAA9224933)

Full Name
CFL1 Antibody (Center) Blocking Peptide
Gene Names
CFL1; CFL; cofilin; HEL-S-15
Western Blot (WB) (Figure 1. Western blot analysis of BMP4 using anti-BMP4 antibody (MBS1750370).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant human BMP4 protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BMP4 at approximately 22KD. The expected band size for BMP4 is at 13KD. )

BMP4, Polyclonal Antibody (Cat# AAA1750370)

Full Name
Anti-BMP4 Picoband Antibody
Gene Names
BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Western blot analysis of CD31 expression in THP1 cell lysate (MBS179680).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PECAM1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PECAM1 )

CD31, Monoclonal Antibody (Cat# AAA179680)

Full Name
Anti-CD31 Rabbit Monoclonal Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human
Applications
FC/FACS, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Stathmin 1 expression in PC-12 cell lysate (MBS179585).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STMN1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STMN1 )

Stathmin 1, Monoclonal Antibody (Cat# AAA179585)

Full Name
Anti-Stathmin 1 Rabbit Monoclonal Antibody
Gene Names
STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL

GPC6, Blocking Peptide (Cat# AAA9218551)

Full Name
GPC6 Antibody (C-term) Blocking peptide
Gene Names
GPC6; OMIMD1
Western Blot (WB) (Western blot analysis of lysates from Jurkat cells, using RIT1 Antibody. The lane on the right is blocked with the synthesized peptide.)

RIT1, Polyclonal Antibody (Cat# AAA9504717)

Full Name
RIT1 Antibody
Gene Names
RIT1; NS8; RIT; RIBB; ROC1
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB) (Western blot analysis of CD59 expression in HUVEC cell lysate (MBS179488).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD59 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD59 )

CD59, Monoclonal Antibody (Cat# AAA179488)

Full Name
Anti-CD59 Rabbit Monoclonal Antibody
Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Reactivity
Human
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of HDAC8 expression in HeLa cell lysate (MBS179749).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC8 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HDAC8 )

HDAC8/Histone Deacetylase 8, Monoclonal Antibody (Cat# AAA179749)

Full Name
Anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal Antibody
Gene Names
HDAC8; HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts from Colo205, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody. The lane on the left was treated with blocking peptide.)

p38 MAPK, Polyclonal Antibody (Cat# AAA9613047)

Full Name
Phospho-p38 MAPK (Thr180/Tyr182) Antibody
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (91%)
Applications
WB, IHC, IF, ICC, IP, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$305/0.1 mL | $365/0.2 mL | $1,495/5x0.2 mL

EPS8, Blocking Peptide (Cat# AAA9227585)

Full Name
EPS8 Antibody (N-term) Blocking Peptide
Gene Names
EPS8; DFNB102
Immunofluorescence (IF) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AP22305c at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).)

BCL2L10, Polyclonal Antibody (Cat# AAA9231241)

Full Name
BCL2L10 Antibody (Center)
Gene Names
BCL2L10; Boo; Diva; BCL-B; bcl2-L-10
Reactivity
Human
Applications
FC/FACS, IF, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB) (Western blot analysis of BCL2A1 expression in Raji cell lysate (MBS179989).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2A1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BCL2A1 )

BCL2A1/Bcl 2 Related Protein A1, Monoclonal Antibody (Cat# AAA179989)

Full Name
Anti-BCL2A1/Bcl 2 Related Protein A1 Rabbit Monoclonal Antibody
Gene Names
BCL2A1; GRS; ACC1; ACC2; BFL1; ACC-1; ACC-2; HBPA1; BCL2L5
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Figure 1. Western blot analysis of Leptin using anti-Leptin antibody (MBS1750419). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant human Leptin protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Leptin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Leptin at approximately 16KD. The expected band size for Leptin is at 16KD. )

Leptin, Polyclonal Antibody (Cat# AAA1750419)

Full Name
Anti-Leptin Picoband antibody
Gene Names
LEP; OB; OBS; LEPD
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, WB
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Western blot analysis of HIF-2 alpha in (1)MCF-7 cell lysate;(2)HeLa cell lysate (MBS179608).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPAS1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EPAS1 )

HIF-2 alpha, Monoclonal Antibody (Cat# AAA179608)

Full Name
Anti-HIF-2 alpha Rabbit Monoclonal Antibody
Gene Names
EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Figure 1. Western blot analysis of HFE using anti-HFE antibody (MBS1750424).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: HELA whole Cell lysates,Lane 2: HEPG2 whole Cell lysates,Lane 3: A431 whole Cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HFE antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HFE at approximately 40KD. The expected band size for HFE is at 40KD. )

HFE, Polyclonal Antibody (Cat# AAA1750424)

Full Name
Anti-HFE Picoband Antibody
Gene Names
HFE; HH; HFE1; HLA-H; MVCD7; TFQTL2
Reactivity
Human
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts of various cells, using ARHGEF3 antibody.)

ARHGEF3, Polyclonal Antibody (Cat# AAA2541580)

Full Name
ARHGEF3 Polyclonal Antibody
Gene Names
ARHGEF3; GEF3; STA3; XPLN
Reactivity
Human
Applications
WB
Purity
Antigen Affinity Purification
Pricing
$405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Western Blot (WB) (Western blot analysis of Mouse liver lysate showing detection of ~71.3 kDa TCF4 protein using Rabbit Anti-TCF4 Polyclonal Antibody (SPC-723). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse liver lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF4 Polyclonal Antibody (SPC-723) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71.3 kDa. Other Band(s): ~45 kDa isoform.)

TCF4, Polyclonal Antibody (Cat# AAA8000458)

Full Name
TCF4 Antibody: HRP
Gene Names
TCF4; E2-2; ITF2; PTHS; SEF2; FECD3; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; bHLHb19
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB) (Western blot analysis of Cytokeratin 18 expression in HeLa cell lysate (MBS179638).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Krt18 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Krt18 )

Cytokeratin 18, Monoclonal Antibody (Cat# AAA179638)

Full Name
Anti-Cytokeratin 18 Rabbit Monoclonal Antibody
Gene Names
Krt18; K18; CK18; Krt1-18
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of HDAC8 expression in HeLa cell lysate (MBS179748).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC8 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HDAC8 )

HDAC8/Histone Deacetylase 8, Monoclonal Antibody (Cat# AAA179748)

Full Name
Anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal Antibody
Gene Names
HDAC8; HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Figure 1. Western blot analysis of MIP-3 Alpha/CCL20 using anti-MIP-3 Alpha/CCL20 antibody (MBS1750473).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat testis tissue lysates,Lane 2: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIP-3 Alpha/CCL20 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MIP-3 Alpha/CCL20 at approximately 14KD. The expected band size for MIP-3 Alpha/CCL20 is at 14KD. )

MIP-3 Alpha/CCL20, Polyclonal Antibody (Cat# AAA1750473)

Full Name
Anti-MIP-3 Alpha/CCL20 Picoband Antibody
Gene Names
Ccl20; CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Western blot analysis: HO-1 was detected using HO-1, pAb (lanes 1, 3, 5 and 7), and HO-2 was detected using HO-2, pAb (lanes 2, 4, 6 and 8). Both antibodies were used at a dilution of 1:1000. Luminograph (ECL, 15 sec. exposure, ref. FM020/054) showing HO-1 and HO-2 expression in lysates prepared from gIFN/LPS stimulated RAW264.7 mouse macrophage cells (lanes 1 and 2), HepG2 (lanes 3 and 4), rat testis (HP9312, lanes 5 and 6) and rat spleen (HP9313, lanes 7 and 8).)

Heme oxygenase 2, Polyclonal Antibody (Cat# AAA565778)

Full Name
Heme oxygenase 2 polyclonal antibody
Gene Names
HMOX2; HO-2
Reactivity
Human, Rat
Applications
IHC, WB
Purity
Protein G-affinity purified.
Pricing
$505/0.1 mL | $2,240/5x0.1 mL
Western Blot (WB) (Western blot analysis of CDH1 (Phospho-Ser844) using PI3-kina H2O2 treated COS7 whole cell lysates.-/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide).)

E-cadherin, Polyclonal Antibody (Cat# AAA9614365)

Full Name
Phospho-E-cadherin (Ser844) Antibody
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (100%), Bovine (91%), Horse (91%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (83%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of Dnmt3b expression in (1) A549 cell lysate; (2) A431 cell lysate (MBS179261).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT3B monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DNMT3B )

Dnmt3b, Monoclonal Antibody (Cat# AAA179261)

Full Name
Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody
Gene Names
DNMT3B; ICF; ICF1; M.HsaIIIB
Reactivity
Human, Mouse, Rat
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast cancer, using BMP4 Antibody(MBS179262)BMP4 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BMP4 Antibody (MBS179262)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

BMP4, Monoclonal Antibody (Cat# AAA179262)

Full Name
Anti-BMP4 Rabbit Monoclonal Antibody
Gene Names
BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts from various samples, using Bub1 Antibody.Lane 1: Hela cells(heat shock treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(heat shock treatment),Lane 3: HepG2 cells(serum starvation treatment).)

Bub1, Polyclonal Antibody (Cat# AAA9613789)

Full Name
Bub1 Antibody
Gene Names
BUB1; BUB1A; BUB1L; hBUB1
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL

P53, Polyclonal Antibody (Cat# AAA9612812)

Full Name
Acetyl-P53 (Lys382) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Applications
EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot detection of Interferon alpha/beta receptor 1 in K562,Hela cell lysates using Interferon alpha/beta receptor 1 Rabbit mAb(1:1000 diluted). Predicted band size:64kDa. Observed band size: 110-130kDa.)

Interferon alpha/beta receptor 1, Monoclonal Antibody (Cat# AAA4752840)

Full Name
Interferon alpha/beta receptor 1 Rabbit mAb
Gene Names
IFNAR1; AVP; IFRC; IFNAR; IFNBR; IFN-alpha-REC
Reactivity
Human
Applications
WB, IP
Purity
Affinity purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Western blot analysis of FMRP expression in K562 cell lysate (MBS179176).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMR1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FMR1 )

FMRP, Monoclonal Antibody (Cat# AAA179176)

Full Name
Anti-FMRP Rabbit Monoclonal Antibody
Gene Names
FMR1; POF; FMRP; POF1; FRAXA
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Calponin expression in Mouse stomach lysate (MBS1750105).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNN1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CNN1 )

Calponin, Monoclonal Antibody (Cat# AAA1750105)

Full Name
Anti-Calponin Rabbit Monoclonal Antibody
Gene Names
CNN1; SMCC; Sm-Calp; HEL-S-14
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts from various samples, using Acetyl-Histone H3 (Lys9/14/18/23/27) Antibody.Lane 1: Heat-shock treated EC304 cells, blocked with antigen-specific peptides,Lane 2: Heat-shock treated EC304 cells,Lane 3: UV treated A2780 cells,Lane 4: UV treated RAW264.7 cells.)

Histone H3, Polyclonal Antibody (Cat# AAA9613166)

Full Name
Acetyl-Histone H3 (Lys9/14/18/23/27) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
$245/0.05 mL | $305/0.1 mL | $365/0.2 mL | $1,495/5x0.2 mL
Western Blot (WB) (Western blot analysis of Myosin 2 expression in mouse skeletal muscle (A), rat skeletal muscle (B) whole cell lysates.)

Myosin 2, Polyclonal Antibody (Cat# AAA8307360)

Full Name
Anti-Myosin 2 Antibody
Gene Names
MYH2; IBM3; MYH2A; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC
Purity
Purified by Immunogen Affinity Chromatography
Pricing
$200/0.05 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL

RELT / TNFRSF19L, Recombinant Protein (Cat# AAA2546197)

Full Name
Recombinant Human RELT / TNFRSF19L Protein (His tag)
Gene Names
RELT; TRLT; TNFRSF19L
Purity
> 95 % as determined by SDS-PAGE
Pricing
$440/0.05 mg | $675/0.1 mg | $3,030/5x0.1 mg
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