Western Blot (WB)
(Western blot analysis using NPC1 mAb against human NPC1 recombinant protein. (Expected MW is 37.6 kDa) (Left). Western blot analysis using NPC1 mAb against HEK293 (1) and NPC1 (AA:34-174)-hIgGFc transfected HEK293 (2) cell lysate (Right).)
Mouse anti-Human NPC1 Monoclonal Antibody | anti-NPC1 antibody
Mouse Monoclonal Antibody to NPC1
Immunohistochemistry (IHC-P): 1/200 - 1/1000
Flow Cytometry (FCM): 1/200 - 1/400
Western Blot (WB):1/500 - 1/2000
Western Blot (WB)
(Western blot analysis using NPC1 mAb against human NPC1 recombinant protein. (Expected MW is 37.6 kDa) (Left). Western blot analysis using NPC1 mAb against HEK293 (1) and NPC1 (AA:34-174)-hIgGFc transfected HEK293 (2) cell lysate (Right).)
Western Blot (WB)
(Western blot analysis using NPC1 mAb against human NPC1 recombinant protein. (Expected MW is 37.6 kDa) (Left). Western blot analysis using NPC1 mAb against HEK293 (1) and NPC1 (AA:34-174)-hIgGFc transfected HEK293 (2) cell lysate (Right).)
Flow Cytometry (FC)
(Flow cytometric analysis of Hela cells using NPC1 mouse mAb (green) and negative control (red).)
Flow Cytometry (FC)
(Flow cytometric analysis of Hela cells using NPC1 mouse mAb (green) and negative control (red).)
Immunohistochemical (IHC)
(Immunohistochemical analysis of paraffinembedded liver cancer tissues using NPC1 mouse mAb with DAB staining.)
Immunohistochemical (IHC)
(Immunohistochemical analysis of paraffinembedded liver cancer tissues using NPC1 mouse mAb with DAB staining.)
ELISA
(Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng).)
ELISA
(Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng).)
NCBI and Uniprot Product Information
Uniprot Description
Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals.
Research Articles on NPC1
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Product Notes
The NPC1 npc1 (Catalog #AAA8527785) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal Antibody to NPC1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NPC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). ELISA (EIA): 1/10000 Immunohistochemistry (IHC-P): 1/200 - 1/1000 Flow Cytometry (FCM): 1/200 - 1/400 Western Blot (WB):1/500 - 1/2000. Researchers should empirically determine the suitability of the NPC1 npc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPC1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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