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EPS8 blocking peptide

EPS8 Antibody (N-term) Blocking Peptide

Gene Names
EPS8; DFNB102
Synonyms
EPS8; EPS8 Antibody (N-term) Blocking Peptide; Epidermal growth factor receptor kinase substrate 8; EPS8 blocking peptide
Ordering
Specificity
The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human EPS8. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Form/Format
The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
Sequence Length
822
Cellular Location
Cytoplasm, cell cortex. Cell projection, ruffle membrane. Cell projection, growth cone. Cell projection, stereocilium. Cell junction, synapse, synaptosome. Note: Localizes at the tips of the stereocilia of the inner and outer hair cells (By similarity). Localizes to the midzone of dividing cells.
Tissue Location
Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Related Product Information for EPS8 blocking peptide
Signaling adapter that controls various cellular protrusions by regulating actin cytoskeleton dynamics and architecture. Depending on its association with other signal transducers, can regulate different processes. Together with SOS1 and ABI1, forms a trimeric complex that participates in transduction of signals from Ras to Rac by activating the Rac- specific guanine nucleotide exchange factor (GEF) activity. Acts as a direct regulator of actin dynamics by binding actin filaments and has both barbed-end actin filament capping and actin bundling activities depending on the context. Displays barbed-end actin capping activity when associated with ABI1, thereby regulating actin-based motility process: capping activity is auto-inhibited and inhibition is relieved upon ABI1 interaction. Also shows actin bundling activity when associated with BAIAP2, enhancing BAIAP2- dependent membrane extensions and promoting filopodial protrusions. Involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration and cancer cell migration and invasion. Acts as a regulator of axonal filopodia formation in neurons: in the absence of neurotrophic factors, negatively regulates axonal filopodia formation via actin-capping activity. In contrast, it is phosphorylated in the presence of BDNF leading to inhibition of its actin-capping activity and stimulation of filopodia formation. Component of a complex with DFNB31 and MYO15A that localizes at stereocilia tips and is required for elongation of the stereocilia actin core. Indirectly involved in cell cycle progression; its degradation following ubiquitination being required during G2 phase to promote cell shape changes.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
62,871 Da
NCBI Official Full Name
Epidermal growth factor receptor kinase substrate 8
NCBI Official Synonym Full Names
epidermal growth factor receptor pathway substrate 8
NCBI Official Symbol
EPS8
NCBI Official Synonym Symbols
DFNB102
NCBI Protein Information
epidermal growth factor receptor kinase substrate 8
UniProt Protein Name
Epidermal growth factor receptor kinase substrate 8
UniProt Gene Name
EPS8
UniProt Entry Name
EPS8_HUMAN

NCBI Description

This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

EPS8: an adaptor protein that is an epidermal growth factor receptor (EGFR) substrate. Enhances EGF-dependent mitogenic signals. Member of a family of actin barbing and capping proteins. Promotes filopodial protrusions. Complexes containing Eps8/Abi1/PI3K/Sos-1 mediate Rac activation following EGFR signaling. Involved in endocytosis of EGFR by binding to the Rab5-specific GTPase activating protein USP6N. Binds to IRSp53, which binds small the small GTPases Rac and Cdc42, and which regulates actin dynamics. Involved in the actin-remodeling activity in cultured cerebellar neurons. Regulates the actin-remodeling activities of NMDA and ethanol in neurons. Eps8 knockout mice show behavioral resistance to ethanol. Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: cell cortex; stereocilium; vesicle

Molecular Function: actin binding; protein binding; Rac GTPase binding; SH3/SH2 adaptor activity

Biological Process: actin crosslink formation; actin filament bundle formation; barbed-end actin filament capping; cell proliferation; epidermal growth factor receptor signaling pathway; exit from mitosis; Rac protein signal transduction; regulation of actin filament length; regulation of cell shape; signal transduction

Disease: Deafness, Autosomal Recessive 102

Research Articles on EPS8

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Product Notes

The EPS8 eps8 (Catalog #AAA9227585) is a Blocking Peptide and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "EPS8, Blocking Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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