Filters
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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
1205 results for "Rat IgG Isotype Control" - showing 550-600
Western Blot (WB)
(Figure 1. Western blot analysis of LY6C using anti-LY6C antibody (MBS1753853).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse RAW264. 7 whole cell lysatesLane 2: mouse ANA-1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-LY6C antigen affinity purified polyclonal antibody (Catalog # MBS1753853) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for LY6C at approximately 14KD. The expected band size for LY6C is at 14KD. )
LY6C, Polyclonal Antibody (Cat# AAA1753853)
Full Name
Anti-LY6C Antibody
Gene Names
Ly6c1; Ly6c; Ly-6C; Ly-6C1; AA682074; AA959465
Reactivity
Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of A549 cells using anti-Human POR antibody.
Overlay histogram showing A549 cells stained with A02166-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human POR Antibody for 30 min at 20 degree C. DyLight® 488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
)
POR, Polyclonal Antibody (Cat# AAA1751406)
Full Name
Anti-Human POR DyLight 488 conjugated Antibody
Gene Names
POR; CPR; CYPOR; P450R
Reactivity
Human
Applications
FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$400/0.05 mg | $1,745/5x0.05 mg
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of K562 cells using anti-Human CYP1A1 antibody.
Overlay histogram showing K562 cells stained with A00471-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human CYP1A1 Antibody for 30 min at 20 degree C. DyLight® 488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
)
CYP1A1, Polyclonal Antibody (Cat# AAA1751247)
Full Name
Anti-Human CYP1A1 DyLight 488 conjugated Antibody
Gene Names
CYP1A1; AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX
Reactivity
Human
Applications
FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$400/0.05 mg | $1,745/5x0.05 mg
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of K562 cells using anti-Human Caspase-3 antibody.
Overlay histogram showing K562 cells stained with A00334-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human Caspase-3 Antibody for 30 min at 20 degree C. DyLight® 488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
)
Caspase-3, Polyclonal Antibody (Cat# AAA1751232)
Full Name
Anti-Human Caspase-3 DyLight 488 conjugated Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human
Applications
FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$400/0.05 mg | $1,745/5x0.05 mg
Flow Cytometry (FC/FACS)
(Staining of C57Bl/6 splenocytes with 0.06 ug of Rat IgG2b Isotype Control (open histogram) or 0.06 ug of CD44 antibody (filled histogram) followed by Anti-Rat IgG (FITC). Total viable cells were used for analysis.)
CD44, Monoclonal Antibody (Cat# AAA5309245)
Full Name
CD44 antibody
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified by affinity chromatography.
Pricing
$240/0.05 mg | $1,075/5x0.05 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PARP using anti-PARP antibody (MBS1753958).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysatesLane 2: human HELA whole cell lysatesLane 3: human DAUDI whole cell lysatesLane 4: human HEK293 whole cell lysatesLane 5: rat testis tissue lysatesLane 6: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- PARP antigen affinity purified monoclonal antibody (Catalog # MBS1753958) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for PARP at approximately 113KD. The expected band size for PARP is at 113KD. )
PARP, Monoclonal Antibody (Cat# AAA1753958)
Full Name
Anti-PARP Antibody (monoclonal, 10E11)
Gene Names
PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of MAG using anti-MAG antibody (MBS1754017).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- MAG antigen affinity purified monoclonal antibody (Catalog # MBS1754017) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for MAG at approximately 100KD. The expected band size for MAG is at 100KD. )
MAG, Monoclonal Antibody (Cat# AAA1754017)
Full Name
Anti-MAG Antibody (monoclonal, 2G11)
Gene Names
MAG; GMA; S-MAG; SIGLEC4A; SIGLEC-4A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of A549 cells using anti-Human Bax antibody.
Overlay histogram showing A549 cells stained with A00183-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human Bax Antibody for 30 min at 20 degree C. DyLight® 488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Bax, Polyclonal Antibody (Cat# AAA1751220)
Full Name
Anti-Human Bax DyLight 488 conjugated Antibody
Gene Names
BAX; BCL2L4
Reactivity
Human
Applications
FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$400/0.05 mg | $1,745/5x0.05 mg
Western Blot (WB)
(Figure 1. Western blot analysis of DR1 using anti-DR1 antibody (MBS1753503).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Sw620 whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human A375 whole cell lysatesLane 4: rat testis tissue lysatesLane 5: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-DR1 antigen affinity purified polyclonal antibody (Catalog # MBS1753503) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for DR1 at approximately 19KD. The expected band size for DR1 is at 19KD. )
DR1, Polyclonal Antibody (Cat# AAA1753503)
Full Name
Anti-DR1 Antibody
Gene Names
DR1; NC2; NC2-BETA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CGAS using anti-CGAS antibody (MBS1753849).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human Caco-2 whole cell lysatesLane 3: human A431 whole cell lysatesLane 4: rat lung tissue lysatesLane 5: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CGAS antigen affinity purified polyclonal antibody (Catalog # MBS1753849) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for CGAS at approximately 62KD. The expected band size for CGAS is at 62KD. )
CGAS, Polyclonal Antibody (Cat# AAA1753849)
Full Name
Anti-CGAS Antibody
Gene Names
MB21D1; cGAS; h-cGAS; C6orf150
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of K562 cells using anti-Human CRM1 antibody (M01180-Dyl488). Overlay histogram showing K562 cells stained with M01180-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Human CRM1 Antibody (M01180-Dyl488, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CRM1, Monoclonal Antibody (Cat# AAA1752066)
Full Name
Anti-Human CRM1 DyLight 488 conjugated Antibody (monoclonal, 5G3)
Gene Names
XPO1; emb; CRM1; exp1
Reactivity
Human
Applications
FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$400/0.05 mg | $1,745/5x0.05 mg
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD31 and A647 conjugated Mouse IgG2a isotype control . Figure B. FITC conjugated Mouse anti Human CD31 and A647 conjugated Mouse CD14 . All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock .)
IgG2a, Negative Control (Cat# AAA225867)
Full Name
Mouse IgG2a Negative Control: Amethyst Orange
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
$385/0.1 mg | $1,575/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PCK1/PEPC using anti-PCK1/PEPC antibody (MBS1753485).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysatesLane 2: rat liver tissue lysatesLane 3: rat kidney tissue lysatesLane 4: mouse liver tissue lysatesLane 5: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PCK1/PEPC antigen affinity purified polyclonal antibody (Catalog # MBS1753485) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PCK1/PEPC at approximately 69KD. The expected band size for PCK1/PEPC is at 69KD. )
PCK1/PEPC, Polyclonal Antibody (Cat# AAA1753485)
Full Name
Anti-PCK1/PEPC Antibody
Gene Names
PCK1; PEPCK1; PEPCKC; PEPCK-C
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of lysates from mouse kidney, mouse thymus, rat lung tissue lysate (from left to right, 20ug/lane), using 302389 (1:1000/lane). A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Dlk1, Polyclonal Antibody (Cat# AAA6455515)
Full Name
Dlk1 (Protein delta Homolog 1, DLK-1, Adipocyte Differentiation inhibitor Protein, Preadipocyte Factor 1, Pref-1, Fetal antigen 1, FA1, Dlk1, Dlk, Pref1, Scp-1) (Biotin)
Gene Names
Dlk1; FA1; ZOG; pG2; DlkI; Peg9; SCP1; DLK-1; Ly107; pref-1; AW742678
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, WB
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$900/0.2 mL | $4,035/5x0.2 mL
Western Blot (WB)
(Western Blot analysis in Ramos cell line lysates (35ug/lane) usingMBS642483. This demonstrates that MBS642483 detected the GUCY2D protein (arrow).)
GUCY2D, Polyclonal Antibody (Cat# AAA6305116)
Full Name
GUCY2D, ID (GUCY2D, CORD6, GUC1A4, GUC2D, RETGC, RETGC1, Retinal guanylyl cyclase 1, Guanylate cyclase 2D, retinal, Rod outer segment membrane guanylate cyclase) (MaxLight 650)
Gene Names
GUCY2D; LCA; CYGD; LCA1; RCD2; CORD5; CORD6; GUC2D; ROSGC; retGC; GUC1A4; RETGC-1; ROS-GC1
Reactivity
Human, Rat
Applications
IHC, WB, FC/FACS
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$880/0.1 mL | $3,945/5x0.1 mL
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of HEPG2 cells using anti-N Cadherin/CDH2 antibody (MBS1753444).Overlay histogram showing HEPG2 cells stained with MBS1753444 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-N Cadherin/CDH2 Antibody (MBS1753444, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
N Cadherin/CDH2, Polyclonal Antibody (Cat# AAA1753444)
Full Name
Anti-N Cadherin/CDH2 Antibody
Gene Names
CDH2; CDHN; NCAD; CD325; CDw325
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of non-muscle Myosin IIB/MYH10 using anti-non-muscle Myosin IIB/MYH10 antibody (MBS1753544).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysatesLane 3: mouse NIH/3T3 whole cell lysatesLane 4: human SKOV3 whole cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-non-muscle Myosin IIB/MYH10 antigen affinity purified polyclonal antibody (Catalog # MBS1753544) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for non-muscle Myosin IIB/MYH10 at approximately 229KD. The expected band size for non-muscle Myosin IIB/MYH10 is at 229KD. )
non-muscle Myosin IIB/MYH10, Polyclonal Antibody (Cat# AAA1753544)
Full Name
Anti-non-muscle Myosin IIB/MYH10 Antibody
Gene Names
MYH10; NMMHCB; NMMHC-IIB
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of TGFBR2 using anti-TGFBR2 antibody (MBS1753978).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysatesLane 2: human A549 whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: human Caco-2 whole cell lysatesLane 5: human Hela whole cell lysatesLane 6: human T-47D whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- TGFBR2 antigen affinity purified monoclonal antibody (Catalog # MBS1753978) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for TGFBR2 at approximately 70-85KD. The expected band size for TGFBR2 is at 70-85KD. )
TGFBR2, Monoclonal Antibody (Cat# AAA1753978)
Full Name
Anti-TGFBR2 Antibody (monoclonal, 2F11)
Gene Names
TGFBR2; AAT3; FAA3; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TGFR-2; TGFbeta-RII
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of SHANK3 using anti-SHANK3 antibody (MBS1753413).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SHANK3 antigen affinity purified polyclonal antibody (Catalog # MBS1753413) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SHANK3 at approximately 180-190KD. The expected band size for SHANK3 is at 185KD. )
SHANK3, Polyclonal Antibody (Cat# AAA1753413)
Full Name
Anti-SHANK3 Antibody
Gene Names
SHANK3; PSAP2; SCZD15; PROSAP2; SPANK-2; DEL22q13.3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Daudi cells with anti-CD74 antibody LN-2 (MBS488743) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of LN-2 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
CD74, Monoclonal Antibody (Cat# AAA488743)
Full Name
Anti-CD74 [LN-2]
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, FC, EIA, IP
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow Cytometry evaluation of CD11c expression on dendritic cells using C2262-57D1)
CD11c, Monoclonal Antibody (Cat# AAA6255481)
Full Name
CD11c (Monocyte/Macrophage, CD11 Antigen-like Family Member C, Complement Component 3 Receptor 4 Subunit, Integrin alphaX, ITGAX, ITAX, Leukocyte Adhesion Glycoprotein p150,95 alpha Chain, Leukocyte Adhesion Receptor p150,95, Leu M5, Leukocyte Surface Ant
Gene Names
Itgax; Cr4; N418; Cd11c; AI449405
Reactivity
Mouse
Applications
FC/FACS, IF, IHC, IP
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
$740/0.1 mL | $3,320/5x0.1 mL
Western Blot (WB)
(Western Blot Positive WB detected in: K562 whole cell lysate, Rabbit Skeletal Muscle tissue, Rabbit Kidney lysate All lanes ENO1 antibody at 1:10000 Secondary Goat polyclonal to mouse IgG at 0.261ug/ml Predicted band size: 47 KDa Observed band size: 47 KDa Exposure time: 1min)
ENO1, Monoclonal Antibody (Cat# AAA7136003)
Full Name
ENO1 Monoclonal Antibody
Gene Names
ENO1; NNE; PPH; MPB1; ENO1L1
Reactivity
Human, Mouse, Rat, Rabbit
Applications
EIA, WB, IF, FC/FACS, IP
Purity
>95%, Protein G purified
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of PFA-fixed HeLa cells. EIF2S1 Mouse Monoclonal Antibody (PCRP-EIF2S1-1C11) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red). )
EIF2S1/EIF-2 alpha (EIF2A), Monoclonal Antibody (Cat# AAA4381844)
Full Name
EIF2S1/EIF-2 alpha (EIF2A) Mouse Monoclonal Antibody [Clone PCRP-EIF2S1-1C11]
Gene Names
EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
Reactivity
Human
Predicted to work in Rat and Zebrafish.
Predicted to work in Rat and Zebrafish.
Applications
FC/FACS
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Flow Cytometry (FC/FACS)
(Flow Cytometry Analysis: (representative lot data) Staining of HeLa cells with 2ug of MSX2 or with 2ug of Mouse IgG2b isotype control.)
MSX2, Monoclonal Antibody (Cat# AAA6242529)
Full Name
MSX2 (Homeobox Protein MSX-2, Homeobox Protein Hox-8, HOX8) (APC)
Gene Names
MSX2; FPP; MSH; PFM; CRS2; HOX8; PFM1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Purified by Protein G Affinity Chromatography
Pricing
$895/0.1 mL | $4,010/5x0.1 mL
Testing Data
(Figure A. A488 conjugated Hamster anti Mouse CD3 and RPE conjugated Rat IgG1 isotype control . Figure B. A488 conjugated Hamster anti Mouse CD3 and RPE conjugated Rat anti Mouse CD22 . Alll experiments performed on mouse splenocytes in the presence of murine Seroblock .)
IgG1, Negative Control (Cat# AAA225869)
Full Name
Rat IgG1 Negative Control: Amethyst Orange
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Pricing
$310/0.1 mg | $1,255/5x0.1 mg
Testing Data
(Figure A. FITC conjugated mouse anti human CD3 and Alexa 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD3 and Alexa 647 conjugated mouse anti human CD19 . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock )
CD19, Monoclonal Antibody (Cat# AAA225849)
Full Name
Mouse Anti Human CD19: Amethyst Orange
Gene Names
CD19; B4; CVID3
Reactivity
Human
Applications
FC/FACS
Purity
Purified IgG from ascites prepared by precipitation and ion exchange chromatography.
Pricing
$500/0.1 mg | $2,090/5x0.1 mg
Western Blot (WB)
(Western Blot detection of COX IV antibody in Goat muscle, CHO-k1, COS7, 3T3, Hela, C6 and K562 cell lysates using COX IV antibody (1:5000 diluted).)
Cox-4, Monoclonal Antibody (Cat# AAA3014279)
Full Name
Cox-4(C-terminus) Monoclonal Antibody
Gene Names
COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1
Reactivity
Human, Mouse, Rat, Monkey, Goat, Hamster
Applications
WB, FC/FACS, ICC, IP
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Pricing
$310/0.05 mL | $435/0.1 mL | $1,695/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of lysates from mouse kidney, mouse thymus, rat lung tissue lysate (from left to right, 20ug/lane), using 302389 (1:1000/lane). A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.)
Dlk1, Polyclonal Antibody (Cat# AAA6455514)
Full Name
Dlk1 (Protein delta Homolog 1, DLK-1, Adipocyte Differentiation inhibitor Protein, Preadipocyte Factor 1, Pref-1, Fetal antigen 1, FA1, Dlk1, Dlk, Pref1, Scp-1) (APC)
Gene Names
Dlk1; FA1; ZOG; pG2; DlkI; Peg9; SCP1; DLK-1; Ly107; pref-1; AW742678
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$900/0.2 mL | $4,035/5x0.2 mL
Western Blot (WB)
(Western Blot analysis of lysates from mouse kidney, mouse thymus, rat lung tissue lysate (from left to right, 20ug/lane), using 302389 (1:1000/lane). A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Dlk1, Polyclonal Antibody (Cat# AAA6455521)
Full Name
Dlk1 (Protein delta Homolog 1, DLK-1, Adipocyte Differentiation inhibitor Protein, Preadipocyte Factor 1, Pref-1, Fetal antigen 1, FA1, Dlk1, Dlk, Pref1, Scp-1) (MaxLight 650)
Gene Names
Dlk1; FA1; ZOG; pG2; DlkI; Peg9; SCP1; DLK-1; Ly107; pref-1; AW742678
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$900/0.1 mL | $4,035/5x0.1 mL
Western Blot (WB)
(Western Blot analysis in Ramos cell line lysates (35ug/lane) usingMBS642483. This demonstrates that MBS642483 detected the GUCY2D protein (arrow).)
GUCY2D, Polyclonal Antibody (Cat# AAA6305111)
Full Name
GUCY2D, ID (GUCY2D, CORD6, GUC1A4, GUC2D, RETGC, RETGC1, Retinal guanylyl cyclase 1, Guanylate cyclase 2D, retinal, Rod outer segment membrane guanylate cyclase) (FITC)
Gene Names
GUCY2D; LCA; CYGD; LCA1; RCD2; CORD5; CORD6; GUC2D; ROSGC; retGC; GUC1A4; RETGC-1; ROS-GC1
Reactivity
Human, Rat
Applications
IHC, WB, FC/FACS
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Western BlotPositive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, Rat spleen tissue, Rabbit kidney tissueAll lanes: ACTB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 42 KDaObserved band size: 42 KDaExposure time: 5min)
ACTB, Monoclonal Antibody (Cat# AAA9018372)
Full Name
ACTB Monoclonal Antibody
Reactivity
Human, Mouse, Rat, Rabbit
Applications
EIA, WB, IHC, IF, FC/FACS, IP
Purity
>95%, Protein A purified
Pricing
$150/0.05 mL | $200/0.1 mL | $900/5x0.1 mL
IgG, Polyclonal Isotype Control (Cat# AAA560998)
Full Name
Rat IgG Purified
Reactivity
Rat
Applications
EIA
Purity
Purified Protein
Pricing
$245/5 mg | $960/5x5 mg
Flow Cytometry (FC/FACS)
(Flow Cytometry Analysis: (representative lot data) Staining of HeLa cells with 2ug of MSX2 or with 2ug of Mouse IgG2b isotype control.)
MSX2, Monoclonal Antibody (Cat# AAA6243594)
Full Name
MSX2 (Homeobox Protein MSX-2, Homeobox Protein Hox-8, HOX8) (FITC)
Gene Names
MSX2; FPP; MSH; PFM; CRS2; HOX8; PFM1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Purified by Protein G Affinity Chromatography
Pricing
$895/0.1 mL | $4,010/5x0.1 mL
Western Blot (WB)
(Western Blot analysis in Ramos cell line lysates (35ug/lane) usingMBS642483. This demonstrates that MBS642483 detected the GUCY2D protein (arrow).)
GUCY2D, Polyclonal Antibody (Cat# AAA6305115)
Full Name
GUCY2D, ID (GUCY2D, CORD6, GUC1A4, GUC2D, RETGC, RETGC1, Retinal guanylyl cyclase 1, Guanylate cyclase 2D, retinal, Rod outer segment membrane guanylate cyclase) (MaxLight 550)
Gene Names
GUCY2D; LCA; CYGD; LCA1; RCD2; CORD5; CORD6; GUC2D; ROSGC; retGC; GUC1A4; RETGC-1; ROS-GC1
Reactivity
Human, Rat
Applications
IHC, WB, FC/FACS
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$880/0.1 mL | $3,945/5x0.1 mL
Western Blot (WB)
(Western Blot analysis in Ramos cell line lysates (35ug/lane) usingMBS642483. This demonstrates that MBS642483 detected the GUCY2D protein (arrow).)
GUCY2D, Polyclonal Antibody (Cat# AAA6305110)
Full Name
GUCY2D, ID (GUCY2D, CORD6, GUC1A4, GUC2D, RETGC, RETGC1, Retinal guanylyl cyclase 1, Guanylate cyclase 2D, retinal, Rod outer segment membrane guanylate cyclase) (Biotin)
Gene Names
GUCY2D; LCA; CYGD; LCA1; RCD2; CORD5; CORD6; GUC2D; ROSGC; retGC; GUC1A4; RETGC-1; ROS-GC1
Reactivity
Human, Rat
Applications
IHC, WB, FC/FACS, EIA
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Western Blot analysis in Ramos cell line lysates (35ug/lane) usingMBS642483. This demonstrates that MBS642483 detected the GUCY2D protein (arrow).)
GUCY2D, Polyclonal Antibody (Cat# AAA6305114)
Full Name
GUCY2D, ID (GUCY2D, CORD6, GUC1A4, GUC2D, RETGC, RETGC1, Retinal guanylyl cyclase 1, Guanylate cyclase 2D, retinal, Rod outer segment membrane guanylate cyclase) (MaxLight 490)
Gene Names
GUCY2D; LCA; CYGD; LCA1; RCD2; CORD5; CORD6; GUC2D; ROSGC; retGC; GUC1A4; RETGC-1; ROS-GC1
Reactivity
Human, Rat
Applications
IHC, WB, FC/FACS
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$880/0.1 mL | $3,945/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of DR6/TNFRSF21 using anti-DR6/TNFRSF21 antibody (MBS1753655).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human Raji whole cell lysatesLane 3: human Sw620 whole cell lysatesLane 4: rat brain tissue lysatesLane 5: rat C6 whole cell lysatesLane 6: mouse brain tissue lysatesLane 7: mouse Raw164. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-DR6/TNFRSF21antigen affinity purified polyclonal antibody (Catalog # MBS1753655) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for DR6/TNFRSF21 at approximately 80-120KD. The expected band size for DR6/TNFRSF21 is at 72KD. )
DR6/TNFRSF21, Polyclonal Antibody (Cat# AAA1753655)
Full Name
Anti-DR6/TNFRSF21 Antibody
Gene Names
TNFRSF21; DR6; CD358; BM-018
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Tubulin alpha using anti-Tubulin alpha antibody (MBS1754034).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human HEK293 whole cell lysatesLane 4: human K562 whole cell lysatesLane 5: rat brain tissue lysatesLane 6: rat C6 whole cell lysatesLane 7: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Tubulin alpha antigen affinity purified monoclonal antibody (Catalog # MBS1754034) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for Tubulin alpha at approximately 56KD. The expected band size for Tubulin alpha is at 56KD. )
Tubulin alpha, Monoclonal Antibody (Cat# AAA1754034)
Full Name
Anti-Tubulin alpha Antibody (monoclonal, 7B12)
Gene Names
TUBA1A; LIS3; TUBA3; B-ALPHA-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of DDX6 using anti-DDX6 antibody (MBS1754031).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human HepG2 whole cell lysatesLane 3: human Sw620 whole cell lysatesLane 4: human Raji whole cell lysatesLane 5: human THP-1 whole cell lysatesLane 6: human Jurkat whole cell lysatesLane 7: rat PC-12 whole cell lysatesLane 8: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- DDX6 antigen affinity purified monoclonal antibody (Catalog # MBS1754031) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for DDX6 at approximately 54KD. The expected band size for DDX6 is at 54KD. )
DDX6, Monoclonal Antibody (Cat# AAA1754031)
Full Name
Anti-DDX6 Antibody (monoclonal, 8G6)
Gene Names
DDX6; P54; RCK; HLR2
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Figure A. RPE conjugated rat anti mouse TER-119 and FITC conjugated Rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse TER-119 and FITC conjugated rat anti mouse CD71 . All experiments performed on mouse bone marrow. Data acquired on the ZE5 Cell Analyzer)
CD71, Monoclonal Antibody (Cat# AAA225662)
Full Name
Rat Anti Mouse CD71: RPE
Gene Names
Tfrc; TR; TFR; p90; CD71; TFR1; Trfr; Mtvr1; 2610028K12Rik; E430033M20Rik
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$500/100 Tests | $2,090/5x100 Tests
Immunohistochemistry (IHC)
(Immunohistochemistry of a mouse lymph node cryosection with C2255-05B followed by IgG (HRP) Goat anti Rat IgG. Low power)
CD4, Monoclonal Antibody (Cat# AAA6252522)
Full Name
CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4, L3T4, LY-4, Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (MaxLight 405)
Reactivity
Human
Applications
FC/FACS, IF, IHC, IP, WB
Purity
Purified by Protein G Affinity Chromatography
Pricing
$865/0.1 mL | $3,885/5x0.1 mL
Testing Data
(Figure A. FITC conjugated mouse anti human CD61 and RPE conjugated mouse IgG1 isotype control Figure B. FITC conjugated mouse anti human CD61 and RPE conjugated mouse anti human CD49b . All experiments performed on human blood gated on platelets. Data acquired on the ZE5 Cell Analyzer)
CD49b, Monoclonal Antibody (Cat# AAA225640)
Full Name
Mouse Anti Human CD49b: RPE
Gene Names
ITGA2; BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
Reactivity
Human
Does not react with: Rat
Does not react with: Rat
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$500/100 Tests | $2,090/5x100 Tests
Western Blot (WB)
(Western Blot analysis of lysates from mouse kidney, mouse thymus, rat lung tissue lysate (from left to right, 20ug/lane), using 302389 (1:1000/lane). A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Dlk1, Polyclonal Antibody (Cat# AAA6455522)
Full Name
Dlk1 (Protein delta Homolog 1, DLK-1, Adipocyte Differentiation inhibitor Protein, Preadipocyte Factor 1, Pref-1, Fetal antigen 1, FA1, Dlk1, Dlk, Pref1, Scp-1) (MaxLight 750)
Gene Names
Dlk1; FA1; ZOG; pG2; DlkI; Peg9; SCP1; DLK-1; Ly107; pref-1; AW742678
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$900/0.1 mL | $4,035/5x0.1 mL
Immunohistochemistry (IHC)
(Figure 1. IHC analysis of CD229 using anti-CD229 antibody. CD229 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD229 antibody. CD229 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD229 antibody. CD229 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD229 antibody. CD229 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD229 antibody. Overlay histogram showing Jurkat cells stained with A05830-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- CD229 antibody. Overlay histogram showing Daudi cells stained with A05830-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- CD229 antibody. Overlay histogram showing U937 cells stained with A05830-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- CD229 Antibody for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CD229/LY9, Polyclonal Antibody (Cat# AAA1751494)
Full Name
Anti-CD229/LY9 Antibody
Gene Names
LY9; hly9; mLY9; CD229; SLAMF3
Reactivity
Human, Mouse, Rat
Applications
IHC, ICC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of MBS626297 in mouse stomach tissue lysates (35ug/lane). PARD3 (arrow) was detected using the purified Pab.)
ASIP, Polyclonal Antibody (Cat# AAA6464256)
Full Name
ASIP, CT (Atypical PKC Isotype-specific-interacting Protein, Bazooka, Baz, CTCL Tumor Antigen se2-5, FLJ21015, Partitioning Defective 3 Homolog, PAR3, PAR-3, PARD3, PARD-3, PAR3alpha, PAR3-alpha, PAR3A, PARD3A, SE2-5L16, SE2-5LT1, SE2-5T2) (FITC)
Gene Names
PARD3; Baz; ASIP; PAR3; PARD-3; PARD3A; SE2-5T2; PPP1R118; SE2-5L16; SE2-5LT1; PAR3alpha
Reactivity
Human, Mouse
Applications
WB, FC/FACS, IHC
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of TSG101 using anti-TSG101 antibody (MBS1753414).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human Colo320 whole cell lysatesLane 3: human Sw620 whole cell lysatesLane 4: human Caco-2 whole cell lysatesLane 5: human Hek293 whole cell lysatesLane 6: human K562 whole cell lysatesLane 7: human HepG2 whole cell lysatesLane 8: human THP-1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-TSG101 antigen affinity purified polyclonal antibody (Catalog # MBS1753414) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for TSG101 at approximately 44KD. The expected band size for TSG101 is at 44KD. )
TSG101, Polyclonal Antibody (Cat# AAA1753414)
Full Name
Anti-TSG101 Antibody
Gene Names
TSG101; TSG10; VPS23
Reactivity
Human, Mouse, Rat
Applications
WB, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of INHA using anti-INHA antibody (MBS1753521).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: rat brain tissue lysatesLane 4: mouse spleen tissue lysatesLane 5: mouse sp2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-INHA antigen affinity purified polyclonal antibody (Catalog # MBS1753521)at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for INHA at approximately 45KD. The expected band size for INHA is at 45KD. )
Inhibin alpha/INHA, Polyclonal Antibody (Cat# AAA1753521)
Full Name
Anti-Inhibin alpha/INHA Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Mouse spleen tissue, Rat spleen tissue, Mouse heart tissue, Rat heart tissue All lanes HSPA8 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 10s)
HSPA8, Monoclonal Antibody (Cat# AAA7136004)
Full Name
HSPA8 Monoclonal Antibody
Gene Names
HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; NIP71; HSPA10
Reactivity
Human, Rat, Mouse
Applications
EIA, WB, IHC, FC/FACS, IP
Purity
>95%, Protein A purified
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of HP1 alpha/CBX5 using anti-HP1 alpha/CBX5 antibody (MBS1754014).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human U87 whole cell lysatesLane 2: human K562 whole cell lysatesLane 3: human Jurkat whole cell lysatesLane 4: rat brain tissue lysatesLane 5: rat PC-12 whole cell lysatesLane 6: mouse brain tissue lysatesLane 7: mouse SP2/0 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- HP1 alpha/CBX5 antigen affinity purified monoclonal antibody (Catalog # MBS1754014) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for HP1 alpha/CBX5 at approximately 22KD. The expected band size for HP1 alpha/CBX5 is at 22KD. )
HP1 alpha/CBX5, Monoclonal Antibody (Cat# AAA1754014)
Full Name
Anti-HP1 alpha/CBX5 Antibody (monoclonal, 8G6)
Gene Names
CBX5; HP1; HP1A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of HDAC5 using anti-HDAC5 antibody (MBS1753412).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human PC-3 whole cell lysatesLane 3: rat liver tissue lysatesLane 4: mouse liver tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-HDAC5 antigen affinity purified polyclonal antibody (Catalog # MBS1753412) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for HDAC5 at approximately 150KD. The expected band size for HDAC5 is at 150KD. )
HDAC5, Polyclonal Antibody (Cat# AAA1753412)
Full Name
Anti-HDAC5 Antibody
Gene Names
HDAC5; HD5; NY-CO-9
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
