Rabbit anti-Human, Mouse ASIP Polyclonal Antibody | anti-ASIP antibody

ASIP, CT (Atypical PKC Isotype-specific-interacting Protein, Bazooka, Baz, CTCL Tumor Antigen se2-5, FLJ21015, Partitioning Defective 3 Homolog, PAR3, PAR-3, PARD3, PARD-3, PAR3alpha, PAR3-alpha, PAR3A, PARD3A, SE2-5L16, SE2-5LT1, SE2-5T2) (FITC)

Cat. Num. AAA6464256

• Gene Names: PARD3; Baz; ASIP; PAR3; PARD-3; PARD3A; SE2-5T2; PPP1R118; SE2-5L16; SE2-5LT1; PAR3alpha
• Reactivity: Human, Mouse
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ASIP; Polyclonal Antibody; CT (Atypical PKC Isotype-specific-interacting Protein; Bazooka; Baz; CTCL Tumor Antigen se2-5; FLJ21015; Partitioning Defective 3 Homolog; PAR3; PAR-3; PARD3; PARD-3; PAR3alpha; PAR3-alpha; PAR3A; PARD3A; SE2-5L16; SE2-5LT1; SE2-5T2) (FITC); Par-3 Partitioning Defective 3 Homolog (C. elegans); anti-ASIP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PARD3. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ASIP antibody: FLISA, Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1329-1356 in the C-terminal region of human PARD3, UniProt Accession #Q8TEW0. Species sequence homology: rat
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS626297 in mouse stomach tissue lysates (35ug/lane). PARD3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted withMBS626297, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of k562 cells using MBS626297 (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ASIP antibody

PARD proteins, which were first identified in C. elegans, are essential for asymmetric cell division and polarized growth, whereas CDC42 (MIM 116952) mediates the establishment of cell polarity. The CDC42 GTPase, which is controlled by nucleotide exchange factors (GEFs; see MIM 606057) and GTPase-activating proteins (GAPs; see MIM 604980), interacts with a large set of effector proteins that typically contain a CDC42/RAC (MIM 602048)-interactive binding (CRIB) domain.

Product Categories/Family for anti-ASIP antibody

Antibodies; Adapter Proteins

References

1. Noda, Y., et.al., Genes Cells 6(2), 107-119 (2001). 2. Beausoleil, S.A., et.al., Proc. Natl. Acad. Sci. USA 101(33), 12130-12135 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 21361831
• NCBI GeneID: 56288
• NCBI Accession #: NP_062565.2
• NCBI GenBank Nucleotide #: NM_019619.3
• UniProt Accession #: Q8TEW0; Q5T2U1; Q5VUA2; Q5VUA3; Q5VWV0; Q5VWV1; Q5VWV3; Q5VWV4; Q5VWV5; Q6IQ47; Q8TCZ9; F5H5T0
• Molecular Weight: 149,695 Da
• NCBI Official Full Name: partitioning defective 3 homolog isoform 1
• NCBI Official Synonym Full Names: par-3 family cell polarity regulator
• NCBI Official Symbol: PARD3
• NCBI Official Synonym Symbols: Baz; ASIP; PAR3; PARD-3; PARD3A; SE2-5T2; PPP1R118; SE2-5L16; SE2-5LT1; PAR3alpha
• NCBI Protein Information: partitioning defective 3 homolog; bazooka; PAR3-alpha; CTCL tumor antigen se2-5; par-3 partitioning defective 3 homolog; par-3 family cell polarity regulator alpha; protein phosphatase 1, regulatory subunit 118; atypical PKC isotype-specific interacting p
• UniProt Protein Name: Partitioning defective 3 homolog
• Protein Family: Agouti-signaling protein
• UniProt Gene Name: PARD3
• UniProt Synonym Gene Names: PAR3; PAR3A; PAR-3; PARD-3; ASIP
• UniProt Entry Name: PARD3_HUMAN

NCBI Description

This gene encodes a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

PAR3-alpha: an adapter protein involved in asymmetrical cell division, and cell polarization processes. Polarity complex proteins, including PARD3, are essential for PIP3-induced receptor tyrosine kinase recycling. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Association with PARD6B may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons. PDZ 1 domain modulates interactions with JAM1, PARD6A and PARD6B; PDZ 2 domain mediates interaction with membranes containing phosphoinositol lipids; PDZ 3 domain regulates interactions with PTEN (via C-terminus). Belongs to the PAR3 family. 10 isoforms of the human protein are produced by alternative splicing. Isoform 2, but not at least isoform 3 interacts with PRKCZ.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p11.21

Cellular Component: internode region of axon; cell-cell adherens junction; protein complex; tight junction; cell soma; apical part of cell; plasma membrane; endomembrane system; spindle; intercellular junction; cell cortex; cytosol; cell junction

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; protein kinase C binding; protein phosphatase binding; phosphatidylinositol 3-phosphate binding

Biological Process: positive regulation of myelination; intercellular junction assembly and maintenance; regulation of actin filament-based process; establishment and/or maintenance of cell polarity; microtubule cytoskeleton organization and biogenesis; cell cycle; myelination in the peripheral nervous system; centrosome localization; axonogenesis; asymmetric cell division; transforming growth factor beta receptor signaling pathway; protein kinase C activation; protein complex assembly; protein targeting to membrane