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Testing Data
(Figure A. A488 conjugated Hamster anti Mouse CD3 and RPE conjugated Rat IgG1 isotype control . Figure B. A488 conjugated Hamster anti Mouse CD3 and RPE conjugated Rat anti Mouse CD22 . Alll experiments performed on mouse splenocytes in the presence of murine Seroblock .)
IgG1 negative control
Rat IgG1 Negative Control: Amethyst Orange
Applications
Flow Cytometry, Functional Assay
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Synonyms
IgG1; Rat IgG1 Negative Control: Amethyst Orange; Rat IgG1 Negative Control antibody; IgG1 negative control
Host
Rat
Isotype
IgG1
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Form/Format
Purified IgG conjugated to Amethyst Orange - Liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Applicable Applications for IgG1 negative control
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: Use 10ul of the suggested working dilution to label 106 cells in 100ul
Immunogen
Horseradish peroxidase
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
1% Bovine Serum Albumin
Buffer Solution
Phosphate Buffered Saline
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Avoid repeated freezing and thawing as this may denature the antibody.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
This product contains a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
This product contains a precipitate we recommend microcentrifugation before use.
Testing Data
(Figure A. A488 conjugated Hamster anti Mouse CD3 and RPE conjugated Rat IgG1 isotype control . Figure B. A488 conjugated Hamster anti Mouse CD3 and RPE conjugated Rat anti Mouse CD22 . Alll experiments performed on mouse splenocytes in the presence of murine Seroblock .)
Testing Data
(Figure A. A488 conjugated Hamster anti Mouse CD3 and RPE conjugated Rat IgG1 isotype control . Figure B. A488 conjugated Hamster anti Mouse CD3 and RPE conjugated Rat anti Mouse CD22 . Alll experiments performed on mouse splenocytes in the presence of murine Seroblock .)
Testing Data
(Figure A. PE-A750 tandem-conjugated Rat anti Mouse CD45R and RPE conjugated Rat IgG1 isotype control . Figure B. PE-A750 tandem-conjugated Rat anti Mouse CD45R and RPE conjugated Rat anti Mouse CD150 . All experiments performed on mouse splenocytes in the presence of murine SeroBlock .)
Testing Data
(Figure A. PE-A750 tandem-conjugated Rat anti Mouse CD45R and RPE conjugated Rat IgG1 isotype control . Figure B. PE-A750 tandem-conjugated Rat anti Mouse CD45R and RPE conjugated Rat anti Mouse CD150 . All experiments performed on mouse splenocytes in the presence of murine SeroBlock .)
Testing Data
(FigureA.FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat IgG1 isotype control . Figure B. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat anti Canine CD8 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(FigureA.FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat IgG1 isotype control . Figure B. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat anti Canine CD8 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. Purified Mouse anti Canine CD45RA detected with Goat anti Mouse IgG1 DyLight 649 and Rat IgG1 FITC isotype control . Figure B. Purified Mouse anti Canine CD45RA detected with Goat anti Mouse IgG1 PE and Rat anti Canine CD8 FITC . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. Purified Mouse anti Canine CD45RA detected with Goat anti Mouse IgG1 DyLight 649 and Rat IgG1 FITC isotype control . Figure B. Purified Mouse anti Canine CD45RA detected with Goat anti Mouse IgG1 PE and Rat anti Canine CD8 FITC . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Related Product Information for IgG1 negative control
Rat IgG1 negative control, a rat monoclonal raised against horseradish peroxidase, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG1 test antibodies to the surface of human and mouse cells in flow cytometry.
Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.
Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.
Product Categories/Family for IgG1 negative control
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Product Notes
The IgG1 (Catalog #AAA225869) is a Negative Control produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IgG1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Researchers should empirically determine the suitability of the IgG1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG1, Negative Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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