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10000 results for " phosphorylation" - showing 500-550


Western Blot (WB) (Western Blot analysis of Hela cells using IGF-IR Polyclonal Antibody at dilution of 1:2000.)

IGF-IR, Polyclonal Antibody (Cat# AAA2536617)

Full Name
IGF-IR Polyclonal Antibody
Gene Names
IGF1R; IGFR; CD221; IGFIR; JTK13
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Western Blot (WB) (Western Blot analysis of Hela cells using p38 Polyclonal Antibody at dilution of 1:1000.)

p38, Polyclonal Antibody (Cat# AAA2534964)

Full Name
p38 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, EIA
Purity
Antigen Affinity Purified
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from COLO205 cells using cofilin(Phospho-Ser3) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

cofilin, Polyclonal Antibody (Cat# AAA9409461)

Full Name
cofilin (Phospho-Ser3) Antibody
Gene Names
CFL1; CFL; cofilin; HEL-S-15
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Immunofluorescence (IF) (MBS9600828 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

Lamin A/C, Polyclonal Antibody (Cat# AAA9600828)

Full Name
Phospho-Lamin A/C (Ser392) Antibody
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western Blot analysis of various cells using Cdc2 Polyclonal Antibody at dilution of 1:2000.)

Cdc2, Polyclonal Antibody (Cat# AAA2537516)

Full Name
Cdc2 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SYK (Phospho-Tyr348) Antibody. The picture on the right is blocked with the phospho peptide.)

SYK, Polyclonal Antibody (Cat# AAA9502397)

Full Name
SYK (Phospho-Tyr348) Antibody
Gene Names
SYK; p72-Syk
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts from Hela cells untreated or treated with EGF, IFN-a using p27Kip1(Phospho-Thr187) Antibody.)

p27Kip1, Polyclonal Antibody (Cat# AAA9406368)

Full Name
p27Kip1 (Phospho-Thr187) Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human
Applications
WB, IHC
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts from 293 cells untreated or treated with PMA using GSK3alpha/beta(Phospho-Tyr279/216) Antibody.)

GSK3alpha/beta, Polyclonal Antibody (Cat# AAA9409340)

Full Name
GSK3alpha/beta(Phospho-Tyr279/216) Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts from JK cells untreated or treated with starvation using Lck(Phospho-Tyr394) Antibody.)

Lck, Polyclonal Antibody (Cat# AAA9411597)

Full Name
Lck (Phospho-Tyr394) Antibody
Gene Names
LCK; LSK; YT16; IMD22; p56lck; pp58lck
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB) (Western Blot analysis of various cells using NF?B-p100 Polyclonal Antibody at dilution of 1:1000.)

NFkappaB-p100, Polyclonal Antibody (Cat# AAA2536361)

Full Name
NFkappaB-p100 Polyclonal Antibody
Gene Names
NFKB2; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IP, IF, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody.)

PTEN, Polyclonal Antibody (Cat# AAA9406752)

Full Name
PTEN (Phospho-Ser380/Thr382/Thr383) Antibody
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Immunohistochemisty (IHC) (MBS9601013 at 1/100 staining Rat colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

PI3-kinase p85 alpha/gamma, Polyclonal Antibody (Cat# AAA9601013)

Full Name
Phospho-PI3-kinase p85 alpha/gamma (Tyr467/199) Antibody
Gene Names
PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IF, ICC, EIA
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (At 1/100 staining rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

I kappaB alpha, Polyclonal Antibody (Cat# AAA9610638)

Full Name
Phospho-I kappaB alpha (Tyr42) Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI; EDAID2
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Sheep, Rabbit, Dog
Applications
WB, IHC, EIA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Immunohistochemistry (IHC) (MBS9600648 at 1/100 staining Mouse skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Atg14, Polyclonal Antibody (Cat# AAA9600648)

Full Name
Phospho-Atg14 (Ser29) Antibody
Gene Names
ATG14; ATG14L; BARKOR; KIAA0831
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Immunohistochemistry (IHC) (At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

DRP1, Polyclonal Antibody (Cat# AAA9610462)

Full Name
Phospho-DRP1 (Ser529) Antibody
Gene Names
DNM1L; DLP1; DRP1; DVLP; EMPF; OPA5; EMPF1; DYMPLE; HDYNIV
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Applications
WB, IHC, EIA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of MITF phosphorylation expression in COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

MITF, Polyclonal Antibody (Cat# AAA9600799)

Full Name
Phospho-MITF (Ser180/73) Antibody
Gene Names
MITF; MI; WS2; CMM8; WS2A; COMMAD; bHLHe32
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, IF, ICC, EIA
Purity
Peptide affinity puriification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

V5-TAG, Monoclonal Antibody (Cat# AAA214958)

Full Name
MOUSE ANTI V5-TAG:HRP
Applications
EIA, WB
Pricing
$490/0.1 mg | $2,060/5x0.1 mg
Western Blot (WB) (Western Blot analysis of 293T cells using PKD1 Polyclonal Antibody at dilution of 1:1000.)

PKD1, Polyclonal Antibody (Cat# AAA2535180)

Full Name
PKD1 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Immunofluorescence (IF) (MBS9606751 staining A2780 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

p27, Polyclonal Antibody (Cat# AAA9606751)

Full Name
p27 Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9600785 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IKK alpha/beta, Polyclonal Antibody (Cat# AAA9600785)

Full Name
Phospho-IKK alpha/beta (Ser180/181) Antibody
Gene Names
CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9600659 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

c-Kit, Polyclonal Antibody (Cat# AAA9600659)

Full Name
Phospho-c-Kit (Tyr721) Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117; MASTC
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB) (Western Blot analysis of various cells with Smad2 Polyclonal Antibody.)

Smad2, Polyclonal Antibody (Cat# AAA2536165)

Full Name
Smad2 Polyclonal Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Antigen Affinity Purified
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of HeLa cells, using Phospho-GSK3B-S9 antibody at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)

GSK3-S9, Antibody (Cat# AAA125636)

Full Name
Phospho-GSK3-S9 Polyclonal Antibody
Reactivity
Human, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Immunofluorescence (IF) (Immunofluorescence analysis of HepG2 cells, using AurB (Phospho-Thr232) Antibody. The picture on the right is blocked with the phospho peptide.)

AurB, Polyclonal Antibody (Cat# AAA9502809)

Full Name
AurB (Phospho-Thr232) Antibody
Gene Names
AURKB; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; ARK-2; STK-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB) (Western Blot analysis of various cells using Tak1 Polyclonal Antibody at dilution of 1:1000.)

Tak1, Polyclonal Antibody (Cat# AAA2537495)

Full Name
Tak1 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL

interleukin 22, Active Protein (Cat# AAA400082)

Full Name
Mouse interleukin 22 - pegylated
Gene Names
IL22; TIFa; IL-21; IL-22; ILTIF; IL-TIF; IL-D110; zcyto18; TIFIL-23
Purity
Greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Pricing
$315/0.01 mg | $1,025/0.05 mg | $1,490/0.1 mg | $5,650/1 mg
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embeddedhuman Colon Carcinoma stained with TACSTD2 / TROP2Mouse Monoclonal Antibody (TACSTD2/2153).)

TACSTD2/TROP2, Monoclonal Antibody (Cat# AAA4380458)

Full Name
TACSTD2/TROP2 (Epithelial Marker)
Gene Names
TACSTD2; EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1
Reactivity
Human. Others not known.
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Western blot analysis of Phospho-Aurora Kinase (Thr288) expression in various lysates)

Aurora Kinase, Polyclonal Antibody (Cat# AAA9600783)

Full Name
Phospho-Aurora Kinase (Thr288) Antibody
Gene Names
AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SGK (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.)

SGK, Polyclonal Antibody (Cat# AAA9502393)

Full Name
SGK (Phospho-Ser422) Antibody
Gene Names
SGK1; SGK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western blot (WB) (Western blot analysis of extracts from THP1 cells, using Phospho-p40phox (Thr154) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with nonphospho-blocking peptide.)

p40phox, Polyclonal Antibody (Cat# AAA9600704)

Full Name
Phospho-p40phox (Thr154) Antibody
Gene Names
NCF4; NCF; CGD3; P40PHOX; SH3PXD4
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB) (Western Blot analysis of Jurkat cells using FoxO3A Polyclonal Antibody at dilution of 1:1000.)

FoxO3A, Polyclonal Antibody (Cat# AAA2536615)

Full Name
FoxO3A Polyclonal Antibody
Gene Names
FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, EIA
Purity
Antigen Affinity Purified
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Western Blot (WB) (Western Blot analysis of 22RV1 cells using HSP27 Polyclonal Antibody at dilution of 1:1000.)

HSP27, Polyclonal Antibody (Cat# AAA2535595)

Full Name
HSP27 Polyclonal Antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Antigen Affinity Purified
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Immunohistochemistry (IHC) (MBS9600812 at 1/200 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Abl, Polyclonal Antibody (Cat# AAA9600812)

Full Name
Phospho-Abl (Tyr412) Antibody
Gene Names
ABL1; ABL; JTK7; p150; c-ABL; v-abl; CHDSKM; c-ABL1; bcr/abl
Reactivity
Human, Mouse, Monkey
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9601085 at 1/200 staining Human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

GATA4, Polyclonal Antibody (Cat# AAA9601085)

Full Name
Phospho-GATA4 (Ser262) Antibody
Gene Names
GATA4; TOF; ASD2; VSD1; TACHD
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9600626 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

JAK1, Polyclonal Antibody (Cat# AAA9600626)

Full Name
Phospho-JAK1 (Tyr1034/Tyr1035) Antibody
Gene Names
JAK1; JTK3; JAK1A; JAK1B
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9601065 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Calcium Sensing Receptor, Polyclonal Antibody (Cat# AAA9601065)

Full Name
Phospho-Calcium Sensing Receptor (Thr888) Antibody
Gene Names
CASR; CAR; FHH; FIH; HHC; EIG8; HHC1; NSHPT; PCAR1; hCasR; GPRC2A; HYPOC1
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9600965 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Stathmin 1, Polyclonal Antibody (Cat# AAA9600965)

Full Name
Phospho-Stathmin 1 (Ser24) Antibody
Gene Names
STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

V5-TAG, Monoclonal Antibody (Cat# AAA210664)

Full Name
MOUSE ANTI V5-TAG:FITC
Applications
IF
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Immunohistochemistry (IHC) (MBS9600864 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. )

c-Jun, Polyclonal Antibody (Cat# AAA9600864)

Full Name
Phospho-c-Jun (Thr91) Antibody
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9600952 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

CD19, Polyclonal Antibody (Cat# AAA9600952)

Full Name
Phospho-CD19 (Tyr531) Antibody
Gene Names
CD19; B4; CVID3
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9600624 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. )

NF kappaB p65, Polyclonal Antibody (Cat# AAA9600624)

Full Name
Phospho-NF kappaB p65 (Ser536) Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, IP, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Anti-c-KIT Antibody (N-term) at 1:1000dilution+ Mouse cerebellum tissue lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 110 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

c-KIT, Polyclonal Antibody (Cat# AAA9204642)

Full Name
c-KIT Antibody (N-term)
Gene Names
KIT; PBT; SCFR; C-Kit; CD117
Reactivity
Human, Monkey
Applications
WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western Blot analysis of 293T cells using XIAP Polyclonal Antibody at dilution of 1:1000.)

XIAP, Polyclonal Antibody (Cat# AAA2534750)

Full Name
XIAP Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL

TGF-alpha (Transforming Growth Factor alpha), Monoclonal Antibody (Cat# AAA4380249)

Full Name
TGF-alpha (Transforming Growth Factor alpha)
Gene Names
TGFA; TFGA
Reactivity
Human, Cow, Rat, Mouse. Others not known.
Applications
FC/FACS, IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunofluorescence (IF) (MBS9601257 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

DARPP-32, Polyclonal Antibody (Cat# AAA9601257)

Full Name
Phospho-DARPP-32 (Thr34) Antibody
Gene Names
PPP1R1B; DARPP32; DARPP-32
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of STAT1 phosphorylation expression in MCF7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

STAT1, Polyclonal Antibody (Cat# AAA9601069)

Full Name
Phospho-STAT1 (Tyr701) Antibody
Gene Names
STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF/ICC, IP, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western Blot analysis of various cells using Cofilin Polyclonal Antibody at dilution of 1:500.)

Cofilin, Polyclonal Antibody (Cat# AAA2538850)

Full Name
Cofilin Polyclonal Antibody
Gene Names
CFL1; CFL; cofilin; HEL-S-15
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from 3T3 cells untreated or treated with UV using Rb(Phospho-Ser780) Antibody.)

Rb, Polyclonal Antibody (Cat# AAA9413308)

Full Name
Rb (Phospho-Ser780) Antibody
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Sequence Information

Laminin Gamma 1 (LAMC1), Recombinant Protein (Cat# AAA2018823)

Full Name
Recombinant Laminin Gamma 1 (LAMC1)
Gene Names
LAMC1; LAMB2
Reactivity
Human
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
> 97%
Pricing
$155/0.01 mg | $280/0.05 mg | $430/0.1 mg | $525/0.2 mg | $1,005/0.5 mg | $1,480/1 mg | $3,625/5 mg | $16,310/5x5 mg

TGF-alpha, Monoclonal Antibody (Cat# AAA438162)

Full Name
TGF-alpha
Gene Names
TGFA; TFGA
Reactivity
Human, Cow, Rat, Mouse.
Applications
EIA, FC/FACS, IF, IP, WB, IH
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
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