Rabbit GSK3alpha/beta Polyclonal Antibody | anti-GSK3alpha/beta antibody

GSK3alpha/beta(Phospho-Tyr279/216) Antibody

Cat. Num. AAA9409340

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: GSK3alpha/beta; Polyclonal Antibody; GSK3alpha/beta(Phospho-Tyr279/216) Antibody; GSK3a/b(Phospho-Tyr279/216) Antibody; Factor A; GSK-3 alpha/beta; kinase GSK3-alpha/beta; anti-GSK3alpha/beta antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of GSK3alpha/beta only when phosphorylated at tyrosine 279/216.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 483

• Applicable Applications for anti-GSK3alpha/beta antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:200
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of tyrosine 279/216 (V-S-Y(p)-I-C) derived from Human GSK3alpha/beta.
• Modification: Phospho-Tyr279/216
• Target Name: GSK3alpha/beta
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells untreated or treated with PMA using GSK3alpha/beta(Phospho-Tyr279/216) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3alpha/beta(Phospho-Tyr279/216) Antibody (left) or the same antibody preincubated with blocking peptide #51301 (right).)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using GSK3alpha/beta(Phospho-Tyr279/216) Antibody.)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed MEF cells using GSK3alpha/beta(Phospho-Tyr279/216) Antibody.)

Related Product Information for anti-GSK3alpha/beta antibody

Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.

Product Categories/Family for anti-GSK3alpha/beta antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 49574532
• NCBI GeneID: 2931
• NCBI Accession #: NP_063937.2
• NCBI GenBank Nucleotide #: NM_019884.2
• UniProt Accession #: P49840; O14959
• Molecular Weight: 50,981 Da
• NCBI Official Full Name: glycogen synthase kinase-3 alpha
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 alpha
• NCBI Official Symbol: GSK3A
• NCBI Protein Information: glycogen synthase kinase-3 alpha
• UniProt Protein Name: Glycogen synthase kinase-3 alpha
• UniProt Gene Name: GSK3A
• UniProt Synonym Gene Names: GSK-3 alpha
• UniProt Entry Name: GSK3A_HUMAN

NCBI Description

This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011]

Uniprot Description

GSK3A: a proline-directed protein kinase of the GSK family. Implicated in the control of several regulatory proteins including glycogen synthase, Myb, and c-Jun. GSK3 and GSK3 have similar functions. GSK3 phophorylates tau, the principal component of neurofibrillary tangles in Alzheimer disease and is required for maximal production of amyloid plaque peptides by secretase. A GSK3 promoter SNP effects progression of bipolar disorder. The GSK3 inhibitor, lithium, is used to treat bipolar disorder and is seen to block plaque formation. GSK3 generally opposes the action of insulin, and GSK3 hyperactivity is thought to contribute to insulin resistant (type II) diabetes. GSK3 also negatively regulates cardiac hypertrophy. A tumor suppressor role is indicated by the oncogenic potential of stabilized -catenin mutants that lack GSK3 phosphorylation sites. Inhibitor: AR-A014418

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; EC 2.7.11.1; EC 2.7.11.26; Kinase, protein; CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: beta-catenin destruction complex; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; tau-protein kinase activity; ATP binding

Biological Process: glycogen metabolic process; epidermal growth factor receptor signaling pathway; nervous system development; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of TOR signaling pathway; cell migration; phosphoinositide-mediated signaling; Wnt receptor signaling pathway; genetic imprinting; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; unfolded protein response; negative regulation of insulin receptor signaling pathway; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; negative regulation of glucose import; negative regulation of transferase activity; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; cellular response to insulin stimulus; positive regulation of cAMP biosynthetic process; positive regulation of protein catabolic process; insulin receptor signaling pathway; regulation of systemic arterial blood pressure; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of heart contraction; negative regulation of glycogen biosynthetic process