Mouse TGF-alpha (Transforming Growth Factor alpha) Monoclonal Antibody | anti-TGFA antibody
TGF-alpha (Transforming Growth Factor alpha)
Gene Names
TGFA; TFGA
Reactivity
Human, Cow, Rat, Mouse. Others not known.
Applications
Flow Cytometry, Functional Assay, Immunofluorescence
Synonyms
TGF-alpha (Transforming Growth Factor alpha); Monoclonal Antibody; EGF-like TGF; ETGF; TFGA; TGF Type 1; TGFA; Wa1; Waved 1; anti-TGFA antibody
Host
Mouse
Reactivity
Human, Cow, Rat, Mouse. Others not known.
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
SPM357
Specificity
This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGFª +/- (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
159
Applicable Applications for anti-TGFA antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
FC/FACS: 0.5-1ug/million cells in 0.1ml
IF: 1-2ug/ml
IF: 1-2ug/ml
Immunogen
A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGFa
Cellular Localization
Cytoplasmic and Extracellular (secreted)
Hu-chromosome Location
2p13.3
Positive Control
Jurkat or Ramos cells. Testicular Carcinoma
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
References
Kobrin MS et. al. J Bio Chem, 1986, 261:14414-9. Kudlow JE et. al. Endocrinology, 1987, 121(4):1577-9. Kobrin MS et. al. Endocrinology, 1987, 121(4):1412-6. Kudlow JE et. al. J Bio Chem, 1989, 264(7):3880-3
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~6kDa
NCBI Official Full Name
protransforming growth factor alpha isoform 2 preproprotein
NCBI Official Synonym Full Names
transforming growth factor alpha
NCBI Official Symbol
TGFA
NCBI Official Synonym Symbols
TFGA
NCBI Protein Information
protransforming growth factor alpha
UniProt Protein Name
Protransforming growth factor alpha
Protein Family
UniProt Gene Name
TGFA
UniProt Synonym Gene Names
TGF-alpha; ETGF
NCBI Description
This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Research Articles on TGFA
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Product Notes
The TGFA tgfa (Catalog #AAA4380249) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TGF-alpha (Transforming Growth Factor alpha) reacts with Human, Cow, Rat, Mouse. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's TGF-alpha (Transforming Growth Factor alpha) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF). FC/FACS: 0.5-1ug/million cells in 0.1ml IF: 1-2ug/ml. Researchers should empirically determine the suitability of the TGFA tgfa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGF-alpha (Transforming Growth Factor alpha), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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