Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse heart using MBS7001480 at dilution 1:50)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta using MBS7001480 at dilution 1:50)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human prostate using MBS7001480 at dilution 1:50)
Western Blot (WB) (Positive WB detected in: A549 whole cell lysateAll lanes: WNT3A antibody at 3.5g/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 40, 43 kDaObserved band size: 40 kDa)
Immunohistochemistry (IHC) (IHC image of MBS7001480 diluted at 1:260 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Western Blot (WB) (All lanes: Protein Wnt-2 antibody at 2ug/mlLane 1:HepG2 whole cell lysateLane 2:MCF-7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 40kDaObserved band size: 40kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human rectum using MBS1497402 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung using MBS1497402 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta using MBS1497402 at dilution 1:100)
SDS-PAGE
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using WNT3A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.)
Western Blot (WB) (Western blotAll lanes: WNT4 antibody at 8ug/ml+ MCF7 whole cell lysateGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 40,33 kDaObserved band size: 39 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis using MBS7004162 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human heart using MBS7004162 at dilution of 1:100)
Western Blot (WB) (Western blotAll lanes: Wnt3 antibody at 2ug/mlLane 1:A431 whole cell lysateLane 2:HepG2 whole cell lysateLane 3:MCF-7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 40kDaObserved band size: 40kDa)
Western Blot (WB) (Western BlotPositive WB detected in: Mouse kidney tissueAll lanes: Wnt3 antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 40 KDaObserved band size: 40 KDa)
SDS-PAGE
Western Blot (WB) (Western blot detection of Wnt3a in MCF-7, ZR75-1, MDA-MB-468 and T47D cell lysates using Wnt3a mouse mAb (1:1000 diluted).Predicted band size: 42KDa.Observed band size:42KDa.)
Immunofluorescence (IF) (MBS9606773 staining HEPG2 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Immunohistochemistry (IHC) (MBS9606773 at 1/100 staining Human lung cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of NCI-H292 lysate using WNT3A antibody. The lane on the left is treated with the antigen-specific peptide.)
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