Typical Testing Data/Standard Curve (for reference only)
Wingless-Type MMTV Integration Site Family, Member 10B ELISA Kit | WNT10B elisa kit
Rat WNT10B ( Wingless-Type MMTV Integration Site Family, Member 10B) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VE-Cadherin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VE-Cadherin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VE-Cadherin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat VE-Cadherin. You can calculate the concentration of Rat VE-Cadherin in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]
Uniprot Description
WNT10B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Defects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6). SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder. Belongs to the Wnt family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: extracellular region; extracellular space; proteinaceous extracellular matrix
Molecular Function: frizzled binding; receptor agonist activity
Biological Process: cell cycle arrest; cell fate commitment; chondrocyte differentiation; G2/M transition of mitotic cell cycle; lipid metabolic process; myoblast cell differentiation involved in skeletal muscle regeneration; negative regulation of epithelial cell proliferation; negative regulation of fat cell differentiation; negative regulation of transcription from RNA polymerase II promoter; neuron differentiation; positive regulation of anagen; positive regulation of apoptosis; positive regulation of bone mineralization; positive regulation of cell proliferation; positive regulation of epithelial cell differentiation; positive regulation of osteoblast differentiation; positive regulation of transcription factor activity; positive regulation of transcriptional preinitiation complex assembly; protein stabilization; regulation of proteasomal ubiquitin-dependent protein catabolic process; regulation of skeletal muscle development; sensory perception of taste; skeletal muscle fiber development; smoothened signaling pathway; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin
Disease: Split-hand/foot Malformation 6
Research Articles on WNT10B
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Product Notes
The Rat WNT10B wnt10b (Catalog #AAA2533600) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533600 ELISA Kit recognizes Rat WNT10B. It is sometimes possible for the material contained within the vial of "Wingless-Type MMTV Integration Site Family, Member 10B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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