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149 results for " ubiquitin e3 Enzymes" - showing 1-50


Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with SUMO-1 Monoclonal Antibody (SPM571))

SUMO-1, Monoclonal Antibody (Cat# AAA438020)

Full Name
SUMO-1 Mouse Monoclonal Antibody
Gene Names
SUMO1; DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3
Reactivity
Human.
Shows broad species reactivity
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Tonsil stained with SUMO-2 Monoclonal Antibody (SUMO2/1199))

SUMO-2, Monoclonal Antibody (Cat# AAA438780)

Full Name
SUMO-2 Mouse Monoclonal Antibody
Gene Names
SUMO3; SMT3A; Smt3B; SMT3H1; SUMO-3
Reactivity
Human, Rat.
Predicted to show a broad species reactivity
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with SUMO-1 Monoclonal Antibody (SUMO1/1188))

SUMO-1, Monoclonal Antibody (Cat# AAA438958)

Full Name
SUMO-1 Mouse Monoclonal Antibody
Gene Names
SUMO1; DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3
Reactivity
Human, Rat.
Predicted to show a broad species reactivity
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with SUMO-2 Monoclonal Antibody (SPM572))

SUMO-2/3, Monoclonal Antibody (Cat# AAA438142)

Full Name
SUMO-2/3 Mouse Monoclonal Antibody
Gene Names
SUMO2; HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
Reactivity
Human.
Shows broad species reactivity
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with SUMO-1 Monoclonal Antibody (SM1/495))

SUMO-1, Monoclonal Antibody (Cat# AAA438277)

Full Name
SUMO-1 Mouse Monoclonal Antibody
Gene Names
SUMO1; DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3
Reactivity
Human, Rat.
Predicted to show a broad species reactivity
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with SUMO-2 Monoclonal Antibody (SM23/496))

SUMO-2/3, Monoclonal Antibody (Cat# AAA438155)

Full Name
SUMO-2/3 Mouse Monoclonal Antibody
Gene Names
SUMO2; HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
Reactivity
Human.
Predicted to show a broad species reactivity
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Tonsil stained with SUMO-2 Monoclonal Antibody (SPM621))

SUMO-2, Monoclonal Antibody (Cat# AAA439221)

Full Name
SUMO-2 Mouse Monoclonal Antibody
Gene Names
SUMO3; SMT3A; Smt3B; SMT3H1; SUMO-3
Reactivity
Human, Rat.
Predicted to show a broad species reactivity
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CDC34 Mouse Monoclonal Antibody (CPTC-CDC34-2).)

Cell Division Cycle 34 homolog, Monoclonal Antibody (Cat# AAA4380753)

Full Name
Cell Division Cycle 34 homolog
Gene Names
CDC34; UBC3; UBCH3; UBE2R1; E2-CDC34
Reactivity
Human. Others not tested.
Applications
Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RNF144B protein (red) or empty vector (blue) stained using RNF144B antibody)

RNF144B, Monoclonal Antibody (Cat# AAA838138)

Full Name
RNF144B antibody
Applications
FC/FACS, IF, WB
Purity
RNF144B antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant MGRN1 protein (red) or empty vector (blue) stained using MGRN1 antibody)

MGRN1, Monoclonal Antibody (Cat# AAA832521)

Full Name
MGRN1 antibody
Gene Names
MGRN1; RNF156
Applications
FC/FACS, IF, WB
Purity
MGRN1 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RNF144B protein (red) or empty vector (blue) stained using RNF144B antibody)

RNF144B, Monoclonal Antibody (Cat# AAA838027)

Full Name
RNF144B antibody
Applications
FC/FACS, IF, WB
Purity
RNF144B antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant MGRN1 protein (red) or empty vector (blue) stained using MGRN1 antibody)

MGRN1, Monoclonal Antibody (Cat# AAA833776)

Full Name
MGRN1 antibody
Gene Names
MGRN1; RNF156
Applications
FC/FACS, IF, WB
Purity
MGRN1 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant MGRN1 protein (red) or empty vector (blue) stained using MGRN1 antibody)

MGRN1, Monoclonal Antibody (Cat# AAA833379)

Full Name
MGRN1 antibody
Gene Names
MGRN1; RNF156
Applications
FC/FACS, IF, WB
Purity
MGRN1 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RNF144B protein (red) or empty vector (blue) stained using RNF144B antibody)

RNF144B, Monoclonal Antibody (Cat# AAA838163)

Full Name
RNF144B antibody
Applications
FC/FACS, IF, WB
Purity
RNF144B antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RNF144B protein (Right) or empty vector (Left) detected with RNF144B antibody)

RNF144B, Monoclonal Antibody (Cat# AAA838533)

Full Name
RNF144B antibody
Applications
FC/FACS, IF, IHC, WB
Purity
RNF144B antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL

Siah E3 Ubiquitin Protein Ligase 1, Recombinant Protein (Cat# AAA145061)

Full Name
Recombinant Human Siah E3 Ubiquitin Protein Ligase 1
Gene Names
SIAH1; SIAH1A
Purity
Greater than 95% as determined by SDS-PAGE.
Pricing
$250/0.01 mg | $5,300/1 mg | $180/0.002 mg | $23,620/5x1 mg
Western Blot (WB) (Western blot using Rockland Immunochemicals affinity purified anti-Ube2j2 antibody shows detection of Ube2j2 in 293 cells over- expressing Myc-Ube2j2 (Lane 1). Lane 2 contains lysate from mock-transfected 293 cells.)

Ube2 J2, Polyclonal Antibody (Cat# AAA619701)

Full Name
Ube2 J2 (Ubiquitin-Conjugating Enzyme E2 J2)
Applications
EL/EIA, WB, IP
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Pricing
$855/0.1 mg | $3,845/5x0.1 mg
SDS-Page

CDC34, Recombinant Protein (Cat# AAA203737)

Full Name
CDC34, 1-236aa, Human, His tag, E Coli
Gene Names
CDC34; UBC3; UBCH3; UBE2R1; E2-CDC34
Applications
SDS-PAGE
Purity
> 95% by SDS-PAGE
Pricing
$215/0.02 mg | $340/0.1 mg | $765/0.5 mg | $3,195/5x0.5 mg
Western Blot (WB) (Western blot using Immunochemicals affinity purified anti-Ube2j1 antibody shows detection of Ube2j1 in 293 cells over- expressing Myc-Ube2j1 (Lane 2). Lane 1 contains lysate from mock- transfected 293 cells.)

Ube2 J1, Polyclonal Antibody (Cat# AAA622985)

Full Name
Ube2 J1 (Ubiquitin-Conjugating Enzyme E2 J1)
Applications
EL/EIA, WB, IP
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Pricing
$855/0.1 mg | $3,845/5x0.1 mg
Western Blot (WB)

ITCH/AIP4, Polyclonal Antibody (Cat# AAA540776)

Full Name
ITCH/AIP4 Antibody Affinity Purified
Gene Names
ITCH; AIF4; AIP4; ADMFD; NAPP1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant UBE2E3 protein (Right) or empty vector (Left) detected with UBE2E3 antibody)

UBE2E3, Monoclonal Antibody (Cat# AAA834862)

Full Name
UBE2E3 antibody
Gene Names
UBE2E3; UBCH9; UbcM2
Applications
WB
Purity
UBE2E3 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (Western Blot analysis using SKP2 antibodyWestern Blot showing SKP2 antibody used against truncated Trx-SKP2 recombinant protein (1) and GST-SKP2 (aa1-130) recombinant protein (2).)

SKP2, Monoclonal Antibody (Cat# AAA830155)

Full Name
SKP2 antibody
Gene Names
SKP2; p45; FBL1; FLB1; FBXL1
Applications
EIA, WB
Pricing
$605/0.1 mL | $2,715/5x0.1 mL
Western Blot (Western Blot analysis using KLHL12 antibodyWestern Blot showing KLHL12 antibody used against Hela (1) cell lysate.)

KLHL12, Monoclonal Antibody (Cat# AAA830473)

Full Name
KLHL12 antibody
Gene Names
KLHL12; DKIR; C3IP1
Applications
EIA, WB
Pricing
$565/0.1 mL | $2,540/5x0.1 mL
Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant UBE2T protein using UBE2T antibody)

UBE2T, Monoclonal Antibody (Cat# AAA838564)

Full Name
UBE2T antibody
Applications
IF, WB
Purity
UBE2T antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL

HECT And RLD Domain Containing E3 Ubiquitin Protein Ligase 5, Recombinant Protein (Cat# AAA146297)

Full Name
Recombinant Human HECT And RLD Domain Containing E3 Ubiquitin Protein Ligase 5
Purity
Greater than 90.0% as determined by SDS-PAGE.
Pricing
$250/0.02 mg | $2,830/1 mg | $180/0.005 mg | $12,520/5x1 mg
Western Blot (WB) (Western blot analysis of Fbx32 using rat kidney tissue lysate.)

Fbx32, Polyclonal Antibody (Cat# AAA837485)

Full Name
Fbx32 antibody
Gene Names
FBXO32; Fbx32; MAFbx
Applications
WB
Purity
Fbx32 antibody was purified by affinity chromatography
Pricing
$480/0.1 mg | $2,145/5x0.1 mg
Immunofluorescence (IF) (Immunofluorescence analysis of HUVEC cells using BAF250B antibody)

BAF250B, Polyclonal Antibody (Cat# AAA5300703)

Full Name
BAF250B antibody
Gene Names
ARID1B; OSA2; 6A3-5; DAN15; MRD12; P250R; BRIGHT; BAF250B; ELD/OSA1
Applications
WB, IF, EIA
Pricing
$405/0.1 mg | $1,805/5x0.1 mg
Western Blot (WB)

MURF1, Polyclonal Antibody (Cat# AAA540507)

Full Name
MURF1 Antibody Affinity Purified
Gene Names
TRIM63; IRF; SMRZ; MURF1; MURF2; RNF28
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Western Blot (Western Blot analysis using HAUSP antibodyWestern Blot showing HAUSP antibody used against MCF-7 (1) cell lysate.)

HAUSP, Monoclonal Antibody (Cat# AAA830027)

Full Name
HAUSP antibody
Gene Names
USP7; TEF1; HAUSP
Applications
EIA, WB
Pricing
$605/0.1 mL | $2,715/5x0.1 mL
Western Blot (WB) (Western Blot showing RNF8 antibody used at a concentration of 1.25 ug/ml against Jurkat Cell Lysate)

RNF8, Polyclonal Antibody (Cat# AAA832873)

Full Name
RNF8 antibody
Gene Names
RNF8; hRNF8
Applications
IHC, WB
Pricing
$535/0.1 mL | $2,395/5x0.1 mL
Western Blot (WB) (Western blot of HEK293 cells transfected with Parkin WT (Phospho) and Parkin S378 mutant (non-phospho) showing the phospho-specific immunolabeling of ~ 52 k parkinprotein)

PARK2, Polyclonal Antibody (Cat# AAA837624)

Full Name
PARK2 antibody (Ser378)
Gene Names
PARK2; PDJ; PRKN; AR-JP; LPRS2
Applications
WB
Purity
PARK2 antibody was purified by affinity chromatography
Pricing
$640/0.1 mL | $2,870/5x0.1 mL
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant MGRN1 protein (red) or empty vector (blue) stained using MGRN1 antibody)

MGRN1, Monoclonal Antibody (Cat# AAA832563)

Full Name
MGRN1 antibody
Gene Names
MGRN1; RNF156
Applications
FC/FACS, WB
Purity
MGRN1 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL

FBXW4 / SHFM3, Polyclonal Antibody (Cat# AAA541576)

Full Name
FBXW4 / SHFM3 Antibody Affinity Purified
Gene Names
FBXW4; DAC; FBW4; FBWD4; SHFM3; SHSF3
Reactivity
Human, Monkey, Mouse, Rat
Applications
DB, EIA, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts from K562 cells and HT-29 cells, using RNF125 antibody.)

RNF125, Polyclonal Antibody (Cat# AAA5301795)

Full Name
RNF125 antibody
Applications
WB, EIA
Pricing
$405/0.1 mg | $1,805/5x0.1 mg

HACE1, Monoclonal Antibody (Cat# AAA6005929)

Full Name
HACE1 (E3 Ubiquitin-protein Ligase HACE1, HECT Domain and Ankyrin Repeat-containing E3 Ubiquitin-protein Ligase 1, KIAA1320)
Reactivity
Human
Applications
EL/EIA
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Pricing
$620/0.1 mg | $2,775/5x0.1 mg
Western Blot (WB) (Western blot of HEK293 cells transfected with Parkin WT (Phospho) and Parkin S101 mutant (non-phospho) showing the phospho-specific immunolabeling of ~ 52 k parkin protein)

PARK2, Polyclonal Antibody (Cat# AAA835520)

Full Name
PARK2 antibody (Ser101)
Gene Names
PARK2; PDJ; PRKN; AR-JP; LPRS2
Applications
WB
Purity
PARK2 antibody was purified by affinity chromatography
Pricing
$640/0.1 mL | $2,870/5x0.1 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of USP5 protein in paraffin embedded Adenocarcinoma of Human colon tissue using USP5 antibody)

USP5, Monoclonal Antibody (Cat# AAA834204)

Full Name
USP5 antibody
Gene Names
USP5; ISOT
Applications
IHC
Purity
USP5 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB) (UBE3B antibody (MBS839805) used at 1 ug/ml to detect target protein.)

UBE3B, Polyclonal Antibody (Cat# AAA839805)

Full Name
UBE3B antibody
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity purified
Pricing
$605/0.1 mL | $2,710/5x0.1 mL
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RNF144B protein (red) or empty vector (blue) stained using RNF144B antibody)

RNF144B, Monoclonal Antibody (Cat# AAA837891)

Full Name
RNF144B antibody
Applications
FC/FACS, IF
Purity
RNF144B antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RNF144B protein (red) or empty vector (blue) stained using RNF144B antibody)

RNF144B, Monoclonal Antibody (Cat# AAA838276)

Full Name
RNF144B antibody
Applications
FC/FACS, IF
Purity
RNF144B antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RNF144B protein (red) or empty vector (blue) stained using RNF144B antibody)

RNF144B, Monoclonal Antibody (Cat# AAA838339)

Full Name
RNF144B antibody
Applications
FC/FACS
Purity
RNF144B antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RNF144B protein (red) or empty vector (blue) stained using RNF144B antibody)

RNF144B, Monoclonal Antibody (Cat# AAA838629)

Full Name
RNF144B antibody
Applications
FC/FACS, IF
Purity
RNF144B antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RNF144B protein (red) or empty vector (blue) stained using RNF144B antibody)

RNF144B, Monoclonal Antibody (Cat# AAA838465)

Full Name
RNF144B antibody
Applications
FC/FACS, IF
Purity
RNF144B antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL

Ubiquitin Conjugating Enzyme E2D2, Recombinant Protein (Cat# AAA147103)

Full Name
Recombinant Human Ubiquitin Conjugating Enzyme E2D2
Gene Names
UBE2D2; UBC4; PUBC1; UBCH4; UBC4/5; UBCH5B; E2(17)KB2
Purity
Greater than 95% as determined by SDS-PAGE
Pricing
$250/0.02 mg | $3,725/1 mg | $180/0.005 mg | $16,535/5x1 mg

UBCH3, Recombinant Protein (Cat# AAA5304565)

Full Name
UBCH3 protein (His tag)
Purity
UBCH3 protein was purified using a a proprietary chromatographic technique.
Pricing
$630/0.1 mg | $2,835/5x0.1 mg
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant RNF144B protein (red) or empty vector (blue) stained using RNF144B antibody)

RNF144B, Monoclonal Antibody (Cat# AAA838419)

Full Name
RNF144B antibody
Applications
FC/FACS, IF
Purity
RNF144B antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB) (Western blot analysis of UBE2I expression in HeLa cell lysate (MBS179824).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2I monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for UBE2I )

UBE2I/Ubc9, Monoclonal Antibody (Cat# AAA179824)

Full Name
Anti-UBE2I/Ubc9 Rabbit Monoclonal Antibody
Gene Names
UBE2I; P18; UBC9; C358B7.1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (RNF178 antibody (MBS5300789) used at 1 ug/ml to detect target protein.)

RNF178, Polyclonal Antibody (Cat# AAA5300789)

Full Name
RNF178 antibody
Applications
WB
Purity
Affinity purified
Western Blot (WB) (RNF178 antibody (MBS839599) used at 1 ug/ml to detect target protein.)

RNF178, Polyclonal Antibody (Cat# AAA839599)

Full Name
RNF178 antibody
Applications
WB
Purity
Affinity purified
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using MARCH3 antibody.)

MARCH3, Polyclonal Antibody (Cat# AAA5302737)

Full Name
MARCH3 antibody
Applications
WB, IHC, EIA
Purity
MARCH3 antibody was purified by affinity chromatography
Pricing
$405/0.1 mg | $1,805/5x0.1 mg
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