Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant MGRN1 protein (red) or empty vector (blue) stained using MGRN1 antibody)
Mouse MGRN1 Monoclonal Antibody | anti-MGRN1 antibody
MGRN1 antibody
IF: 1:100
WB: 1:500-2000
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant MGRN1 protein (red) or empty vector (blue) stained using MGRN1 antibody)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant MGRN1 protein (red) or empty vector (blue) stained using MGRN1 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MGRN1 protein (Right) or empty vector (Left) detected with MGRN1 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MGRN1 protein (Right) or empty vector (Left) detected with MGRN1 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant MGRN1 protein using MGRN1 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant MGRN1 protein using MGRN1 antibody)
NCBI and Uniprot Product Information
NCBI Description
Mahogunin (MGRN1) is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro.[supplied by OMIM, Apr 2004]
Uniprot Description
MGRN1: E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to- lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.19; Ligase; EC 6.3.2.-; Cytoskeletal; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: membrane; cytoplasm; early endosome; plasma membrane; cytosol; nucleus
Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: protein monoubiquitination; negative regulation of G-protein coupled receptor protein signaling pathway; endosome to lysosome transport
Research Articles on MGRN1
Similar Products
Product Notes
The MGRN1 mgrn1 (Catalog #AAA833776) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MGRN1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). FC: 1:100 IF: 1:100 WB: 1:500-2000. Researchers should empirically determine the suitability of the MGRN1 mgrn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGRN1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
