Mouse SUMO-2/3 Monoclonal Antibody | anti-SUMO2 antibody

SUMO-2/3 Mouse Monoclonal Antibody

Cat. Num. AAA438142

• Gene Names: SUMO2; HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
• Reactivity: Human.; Shows broad species reactivity
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Synonyms: SUMO-2/3; Monoclonal Antibody; SUMO-2/3 Mouse Monoclonal Antibody; HSMT3; Sentrin 2; Small ubiquitin like modifier 2; Small ubiquitin like modifier protein 3; SMT3A; SMT3B; SMT3 homolog 1 (SMT3H1); SMT3 homolog 2 (SMT3H2); SMT3 homolog; SMT3 suppressor of mif two 3 homolog 1; SMT3 suppressor of mif two 3 homolog 2; SMT3 suppressor of mif two 3 homolog 3; Suppressor of mif two 3 homolog 2; Suppressor of mif two 3 homolog 3; Ubiquitin like protein SMT3A; Ubiquitin like protein SMT3B; anti-SUMO2 antibody

• Host: Mouse
• Reactivity: Human.; Shows broad species reactivity
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SPM572
• Specificity: This MAb reacts with both SUMO-2 and SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, 2 and 3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include MDM2, p53, PML and RanGap1. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1 and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer s disease.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 71

• Applicable Applications for anti-SUMO2 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Western Blot (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Predominantly nuclear with some cytoplasmic
• Immunogen: Recombinant human SUMO2 protein
• Hu-Chromosome Location: 17q25.1 (SUMO-2) & 21q22.3 (SUMO-3)
• Positive Control: HeLa cells or breast carcinoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with SUMO-2 Monoclonal Antibody (SPM572))

NCBI and Uniprot Product Information

• NCBI GI #: 54792071
• NCBI GeneID: 6613
• NCBI Accession #: NP_001005849.1
• NCBI GenBank Nucleotide #: NM_001005849.1
• UniProt Accession #: P61956; P55855; Q32Q42; Q6IPZ6; Q96HK1; B2R4I2
• Molecular Weight: 11-13kDa
• NCBI Official Full Name: small ubiquitin-related modifier 2 isoform b
• NCBI Official Synonym Full Names: small ubiquitin-like modifier 2
• NCBI Official Symbol: SUMO2
• NCBI Official Synonym Symbols: HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
• NCBI Protein Information: small ubiquitin-related modifier 2
• UniProt Protein Name: Small ubiquitin-related modifier 2
• Protein Family: Small ubiquitin-related modifier
• UniProt Gene Name: SUMO2
• UniProt Synonym Gene Names: Smt3B
• UniProt Entry Name: SUMO2_HUMAN

NCBI Description

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

SUMO2: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. Homotrimer (Potential). Crystal packing analysis suggests a possible trimeric assembly, of which the biological significance remains to be determined. Interacts with SAE2 and UBE2I. Covalently attached to a number of proteins. Interacts with PELP1. Interacts with USP25; the interaction sumoylates USP25. Broadly expressed. Belongs to the ubiquitin family. SUMO subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: nucleoplasm; nucleus; PML body

Molecular Function: protein binding; protein tag; ubiquitin protein ligase binding

Biological Process: positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein sumoylation