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1748 results for " calpain" - showing 1-50
Calpain 1, Large Subunit, ELISA Kit (Cat# AAA90660)
Full Name
Fish Calpain 1, Large Subunit ELISA Kit
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Fish
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
SDS-Page
Calpain-2 catalytic subunit (CAPN2), Recombinant Protein (Cat# AAA959275)
Full Name
Recombinant Human Calpain-2 catalytic subunit (CAPN2)
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,415/1 mg (E-Coli) | $2,990/1 mg (Yeast) | $4,235/1 mg (Baculovirus) | $2,190/0.1 mg (Baculovirus) | $2,775/0.1 mg (Mammalian-Cell) | $395/0.1 mg (E-Coli) | $1,505/0.1 mg (Yeast) | $6,475/0.5 mg (Mammalian-Cell) | $1,520/0.02 mg (Baculovirus) | $1,785/0.02 mg (Mammalian-Cell) | $245/0.02 mg (E-Coli) | $1,280/0.02 mg (Yeast)
Calpain, Small Subunit 1, ELISA Kit (Cat# AAA99485)
Full Name
Human Calpain, Small Subunit 1 ELISA Kit
Reactivity
Human
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Calpain-2 catalytic subunit, Recombinant Protein (Cat# AAA9420066)
Full Name
Recombinant Human Calpain-2 catalytic subunit
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Human
Purity
Greater than 90% as determined by SDS-PAGE.
Pricing
$285/0.02 mg | $470/0.1 mg | $1,785/1 mg | $7,885/5x1 mg
Calpain 2, Large Subunit, ELISA Kit (Cat# AAA76340)
Full Name
Human Calpain 2, Large Subunit ELISA Kit
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Human
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
CAST, ELISA Kit (Cat# AAA2503653)
Full Name
Mouse CAST (Calpastatin) ELISA Kit
Gene Names
CAST; BS-17
Reactivity
Mouse
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Western Blot (WB)
(Western blotAll lanes: CAPN2 antibody at 15ug/mlLane 1: Mouse lung tissueLane 2: 3T3 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: A431 whole cell lysateLane 5: Hela whole cell lysateLane 6: A549 whole cell lysateLane 7: HepG-2 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 80,72 kDaObserved band size: 80,42 kDa)
Calpain-2 catalytic subunit, Polyclonal Antibody (Cat# AAA1491556)
Full Name
Rabbit anti-human Calpain-2 catalytic subunit polyclonal Antibody
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Human
Applications
EIA, WB
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Calpain 10, ELISA Kit (Cat# AAA65278)
Full Name
Human Calpain 10 ELISA Kit
Reactivity
Human
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Western Blot (WB)
(Western Blot: Sample: Human HCT116 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human CAPN9 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Calpain 9, Polyclonal Antibody (Cat# AAA2033864)
Full Name
Polyclonal Antibody to Calpain 9 (CAPN9)
Gene Names
CAPN9; GC36; nCL-4
Reactivity
Human
Applications
WB, ICC, IHC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
$165/0.1 mL | $205/0.2 mL | $345/0.5 mL | $425/1 mL | $1,220/5x1 mL
Western Blot (WB)
(Western blotAll lanes: CAPN1 antibody at 6ug/mlLane 1: A375 whole cell lysateLane 2: 293T whole cell lysateLane 3: PC-3 whole cell lysateLane 4: Jurkat whole cell lysateLane 5: Hela whole cell lysateLane 6: A2780 whole cell lysateLane 7: A431 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 82 kDaObserved band size: 82 kDa)
Calpain-1 catalytic subunit, Polyclonal Antibody (Cat# AAA1493523)
Full Name
Rabbit anti-human Calpain-1 catalytic subunit polyclonal Antibody(CAPN1)
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blotAll lanes: Calpastatin antibody at 10ug/mlLane 1: K562 whole cell lysateLane 2: Hela whole cell lysateLane 3: A549 whole cell lysateLane 4: HepG-2 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 77,76,64,72,82,85,83,75,81 kDaObserved band size: 77 kDa)
Calpastatin, Polyclonal Antibody (Cat# AAA7002669)
Full Name
Rabbit anti-human Calpastatin polyclonal Antibody
Gene Names
CAST; BS-17; PLACK
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(All lanes: Calpain-1 catalytic subunit antibody at 2ug/mlLane 1:A431 whole cell lysateLane 2:Hela whole cell lysateLane 3:Jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 82kDaObserved band size: 82kDa)
Calpain-1 catalytic subunit, Polyclonal Antibody (Cat# AAA1491955)
Full Name
Rabbit anti-human Calpain-1 catalytic subunit polyclonal Antibody(CAPN1)
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
calpain 9, ELISA Kit (Cat# AAA9327859)
Full Name
Human Calpain-9, CAPN9 ELISA Kit
Gene Names
CAPN9; GC36; nCL-4
Reactivity
Human
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Western Blot (WB)
(Western Blot analysis of Human normal liver and Mouse liver tissue, Mouse intestines and pancreas tissue, hepg2 and HT-29 cell, Mouse heart tissue using CAPN7 Polyclonal Antibody at dilution of 1:200)
CAPN7, Polyclonal Antibody (Cat# AAA2527313)
Full Name
CAPN7 Polyclonal Antibody
Gene Names
CAPN7; PALBH; CALPAIN7
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen affinity purification
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL
Western Blot (WB)
(Western blotAll lanes: CAPNS1antibody at 1.15ug/mlLane 1 : Mouse lung tissueLane 2 : Mouse brain tissueLane 3 : Hela whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 28 kDaObserved band size: 28 kDa)
CAPNS1, Polyclonal Antibody (Cat# AAA7044855)
Full Name
CAPNS1 Antibody
Gene Names
CAPNS1; CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4
Reactivity
Human, Mouse
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
SDS-PAGE
Calpain-1 catalytic subunit (CAPN1), Recombinant Protein (Cat# AAA957983)
Full Name
Recombinant Human Calpain-1 catalytic subunit (CAPN1)
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,415/1 mg (E-Coli) | $3,065/1 mg (Yeast) | $4,315/1 mg (Baculovirus) | $2,185/0.1 mg (Baculovirus) | $2,820/0.1 mg (Mammalian-Cell) | $395/0.1 mg (E-Coli) | $1,540/0.1 mg (Yeast) | $6,520/0.5 mg (Mammalian-Cell) | $1,550/0.02 mg (Baculovirus) | $1,815/0.02 mg (Mammalian-Cell) | $245/0.02 mg (E-Coli) | $1,310/0.02 mg (Yeast)
CAPN3, cDNA Clone (Cat# AAA1266191)
Full Name
CAPN3 cDNA Clone
Gene Names
CAPN3; p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
Typical Testing Data/Standard Curve (for reference only)
calpain 1, ELISA Kit (Cat# AAA2533369)
Full Name
Human CAPN1 (calpain 1) ELISA Kit
Reactivity
Human
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
CAPN2, ELISA Kit (Cat# AAA2516029)
Full Name
Rat CAPN2(Calpain-2 catalytic subunit)ELISA Kit
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Rat
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human ovarian cancer using MBS7051813 at dilution of 1:100)
CAPN14, Polyclonal Antibody (Cat# AAA7051813)
Full Name
CAPN14 Antibody
Reactivity
Human
Applications
EIA, IHC, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blotAll lanes: CAPNS2 antibody at 8ug/ml+Mouse heart tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 28 kDaObserved band size: 28 kDa)
CAPNS2, Polyclonal Antibody (Cat# AAA7050739)
Full Name
CAPNS2 Antibody
Reactivity
Human, Mouse
Applications
EIA, WB
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Typical Standard Curve/Testing Data
Claudin 3 (CLDN3), ELISA Kit (Cat# AAA451933)
Full Name
Human Claudin 3 (CLDN3) ELISA Kit
Gene Names
CLDN3; RVP1; HRVP1; C7orf1; CPE-R2; CPETR2
Reactivity
Human
Pricing
$430/48-Strip-Wells | $575/96-Strip-Wells | $2,580/5x96-Strip-Wells | $5,040/10x96-Strip-Wells
Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysat,K562 whole cell lysateAll lanes: CAPN1 antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 82 KDaObserved band size: 82 KDa)
CAPN1, Polyclonal Antibody (Cat# AAA7051809)
Full Name
CAPN1 Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; SPG76; CANPL1; muCANP
Reactivity
Human
Applications
EIA, WB, IHC
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)
CAPN1, ELISA Kit (Cat# AAA2515912)
Full Name
Rat CAPN1(calpain 1) ELISA Kit
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Rat
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Western Blot (WB)
(Western blotAll lanes: CAPN6 antibody at 5ug/ml+Human placenta tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 75 kDaObserved band size: 75 kDa)
CAPN6, Polyclonal Antibody (Cat# AAA7004160)
Full Name
CAPN6 Antibody
Gene Names
CAPN6; CANPX; CAPNX; CalpM; DJ914P14.1
Reactivity
Human
Applications
EIA, WB, IHC
Purity
>95%,Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
SDS-Page
(Gel: 8%SDS-PAGELysate: 40 ΞOgLane 1-2: Jurkat and A549 cell lysatesPrimary antibody: CAPN14 Antibody at dilution 1/1000Secondary antibody: Goat anti rabbit IgG at 1/5000 dilutionExposure time: 5 minutes)
CAPN14, Polyclonal Antibody (Cat# AAA9429546)
Full Name
CAPN14 Antibody
Reactivity
Human
Applications
WB, IHC
Purity
Antigen affinity purification
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Activity Data
(Figure. The binding activity of CAPN3 with TTN.Calpain 3 is a calcium-dependent cysteine protease mainly expressed in skeletal muscle. In humans, calpain 3 is encoded by the CAPN3 gene. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. Besides, Titin (TTN) has been identified as an interactor of CAPN3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CAPN3 and recombinant human TTN. Briefly, CAPN3 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TTN-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CAPN3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CAPN3 and TTN was shown in Figure 1, and this effect was in a dose dependent manner.)
Calpain 3 (CAPN3), Active Protein (Cat# AAA2105179)
Full Name
Active Calpain 3 (CAPN3)
Gene Names
CAPN3; p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A; LGMDD4; LGMDR1
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$225/0.01 mg | $485/0.05 mg | $755/0.1 mg | $935/0.2 mg | $1,820/0.5 mg | $2,695/1 mg | $6,585/5 mg
Western Blot (WB)
(Anti-Calpain 1 antibody, MBS175459, Western blottingLane 1: HT1080 Cell LysateLane 2: COLO320 Cell Lysate )
Calpain 1, Polyclonal Antibody (Cat# AAA175459)
Full Name
Anti-Calpain 1 antibody
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from A549,SK-BR-3,mouse C2C12 cell line (from left to right), using CAPN2 Antibody (MBS9231851). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
CAPN2, Monoclonal Antibody (Cat# AAA9231851)
Full Name
CAPN2 Antibody
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Human, Rat
Predicted: Mouse
Predicted: Mouse
Applications
WB, IHC-P, FC/FACS, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells using CAPN1 Antibody(green) compared to an isotype control of mouse IgG1(blue). MBS9200385 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
CAPN1, Monoclonal Antibody (Cat# AAA9200385)
Full Name
CAPN1 Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human
Applications
WB, EIA, FC/FACS, IHC
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Activity Data
(Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Calpain 1, Large Subunit (CAPN1), Active Protein (Cat# AAA2097301)
Full Name
Active Calpain 1, Large Subunit (CAPN1)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$220/0.01 mg | $465/0.05 mg | $710/0.1 mg | $880/0.2 mg | $1,715/0.5 mg | $2,555/1 mg | $6,250/5 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Calpain 1 Mouse Monoclonal Antibody (CAPN1/1530).)
Calpain 1, Monoclonal Antibody (Cat# AAA4381357)
Full Name
Calpain 1
Gene Names
CAPN1; CANP; muCL; CANP1; SPG76; CANPL1; muCANP
Reactivity
Human
Applications
EIA, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(MBS9606965 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
CAPN1, Polyclonal Antibody (Cat# AAA9606965)
Full Name
CAPN1 Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; SPG76; CANPL1; muCANP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from LOVO cells, using CAPN10 antibody.)
CAPN10, Polyclonal Antibody (Cat# AAA9605322)
Full Name
CAPN10 Antibody
Gene Names
CAPN10; CANP10; NIDDM1
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
SDS-Page
(Gel: 6%SDS-PAGE lysate: 40 ¦Œg, Lane: 231 cell lysate, Primary antibody: MBS9429547£¨CAPN15 Antibody) at dilution 1/450 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
CAPN15, Polyclonal Antibody (Cat# AAA9429547)
Full Name
CAPN15 Antibody
Gene Names
CAPN15; SOLH
Reactivity
Human
Applications
WB, IHC
Purity
Antigen affinity purification
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Serum; Lane2: Mouse Placenta Tissue.)
Calpain 1, Large Subunit (CAPN1), Polyclonal Antibody (Cat# AAA2002191)
Full Name
Polyclonal Antibody to Calpain 1, Large Subunit (CAPN1)
Gene Names
Capn1; Capa1; Capa-1; mu-calpin
Reactivity
Mouse
Applications
ICC, IHC, EIA, WB
Purity
Affinity Chromatography
Pricing
$160/0.1 mL | $205/0.2 mL | $340/0.5 mL | $415/1 mL | $1,205/5x1 mL
Immunofluorescence (IF)
(MBS9605323 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CAPN11, Polyclonal Antibody (Cat# AAA9605323)
Full Name
CAPN11 Antibody
Gene Names
CAPN11; calpain11
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).)
Calpastatin, Monoclonal Antibody (Cat# AAA4381359)
Full Name
Calpastatin
Gene Names
CAST; BS-17; PLACK
Reactivity
Human
Applications
EIA, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot analysis of CAPN3 using NIH-3T3 whole cell lysates)
CAPN3, Polyclonal Antibody (Cat# AAA9609637)
Full Name
CAPN3 Antibody
Gene Names
CAPN3; p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A; LGMDD4; LGMDR1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry - Paraffin (IHC)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
CAPN2 / Calpain 2 / M-Calpain, Monoclonal Antibody (Cat# AAA249312)
Full Name
Anti-CAPN2 / Calpain 2 / M-Calpain Antibody (clone 1F4) IHC-plus
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Mouse, Rat, Human, Monkey
Applications
IHC, WB, FC/FACS
Purity
Immunoaffinity purified
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Western Blot (WB)
(Western blot analysis of CAPNS2 using 293 whole cell lysates)
CAPNS2, Polyclonal Antibody (Cat# AAA9609635)
Full Name
CAPNS2 Antibody
Gene Names
CAPNS2; CSS2
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CAPN9 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
CAPN9, Polyclonal Antibody (Cat# AAA9133802)
Full Name
CAPN9 Polyclonal Antibody
Gene Names
CAPN9; GC36; nCL-4
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CAPN3 Antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
CAPN3, Polyclonal Antibody (Cat# AAA9131917)
Full Name
CAPN3 Polyclonal Antibody
Gene Names
CAPN3; p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of CAPN2 using RAW264.7 whole lysates)
CAPN2, Polyclonal Antibody (Cat# AAA9608370)
Full Name
CAPN2 Antibody
Gene Names
CAPN2; CANP2; mCANP; CANPL2; CANPml
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data
(Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Calpain 1, Large Subunit (CAPN1), Active Protein (Cat# AAA2097302)
Full Name
Active Calpain 1, Large Subunit (CAPN1)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$220/0.01 mg | $465/0.05 mg | $710/0.1 mg | $880/0.2 mg | $1,715/0.5 mg | $2,555/1 mg | $6,250/5 mg
Western Blot (WB)
(Western Blot analysis of 293T cell using CAPN1 Polyclonal Antibody at dilution of 1:450)
CAPN1, Polyclonal Antibody (Cat# AAA2517559)
Full Name
CAPN1 Polyclonal Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen affinity purification
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CAPN3 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)
CAPN3, Polyclonal Antibody (Cat# AAA127766)
Full Name
CAPN3
Gene Names
CAPN3; p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunofluorescence (IF)
(MBS9608410 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CAPNS1, Polyclonal Antibody (Cat# AAA9608410)
Full Name
CAPNS1 Antibody
Gene Names
CAPNS1; CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of CAPN6 using NIH-3T3 whole cell lysates)
CAPN6, Polyclonal Antibody (Cat# AAA9609638)
Full Name
CAPN6 Antibody
Gene Names
CAPN6; CANPX; CAPNX; CalpM; DJ914P14.1
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Human Blood Cells; Lane3: Human A431 Cells; Lane4: Mouse Spleen Tissue.)
Calpain, Small Subunit 1, Polyclonal Antibody (Cat# AAA2032788)
Full Name
Calpain, Small Subunit 1 (CAPNS1) Polyclonal Antibody
Gene Names
CAPNS1; CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4
Reactivity
Human
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$165/0.1 mL | $205/0.2 mL | $345/0.5 mL | $425/1 mL | $1,220/5x1 mL
