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2525 results for "protein-decapentaplegic" - showing 1-50

Immunohistochemistry (IHC) (MBS9601136 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Smad2/3, Polyclonal Antibody (Cat# AAA9601136)

Full Name
Phospho-Smad2/3 (Thr8) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide affinity columns
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Typical Testing Data/Standard Curve (for reference only)

Mothers Against Decapentaplegic Homolog 2, ELISA Kit (Cat# AAA2533438)

Full Name
Human Smad2/MADH2 (Mothers Against Decapentaplegic Homolog 2) ELISA Kit
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Western Blot (WB) (All lanes: Mothers against decapentaplegic homolog 9 antibody at 2ug/ml+293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 53,49 kDaObserved band size: 52 kDa)

Mothers against decapentaplegic homolog 9, Polyclonal Antibody (Cat# AAA1495550)

Full Name
Rabbit anti-human Mothers against decapentaplegic homolog 9 polyclonal Antibody(SMAD9)
Gene Names
SMAD9; PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Typical Testing Data/Standard Curve (for reference only)

Mothers Against Decapentaplegic Homolog 2, ELISA Kit (Cat# AAA2533604)

Full Name
Rat Smad2 (Mothers Against Decapentaplegic Homolog 2) ELISA Kit
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Rat
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Western Blot (WB) (Western blotAll lanes: Actin, cytoplasmic 1 antibody at 2ug/ml+PC-3 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 49,44,36,26 kDaObserved band size: 48 kDa)

Mothers against decapentaplegic homolog 3, Polyclonal Antibody (Cat# AAA7000752)

Full Name
Rabbit anti-human Mothers against decapentaplegic homolog 3 polyclonal Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human
Applications
EIA, WB
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Activity Data (Anti-Müllerian hormone (AMH), also named Müllerian inhibiting substance (MIS) belongs to a tissue-specific TGF-beta superfamily growth factor. It can be expressed by male sertoli cells and postnatal testis, and ovarian granulosa cells of females postpartum. AMH expression is critical to sex differentiation at a specific time during fetal development, it appears to be tightly regulated by SF1, GATA factors, DAX1 and FSH. AMH signals through a characteristic receptor consisting of a type I and a type II receptor serine/threonine kinase. Especially the type II receptor is unique and specific receptor for AMH. Besides, Mothers Against Decapentaplegic Homolog 9 (Smad9) has been identified as an interactor of AMH, thus a binding ELISA assay was conducted to detect the interaction of recombinant human AMH and recombinant human (Smad9) Briefly, AMH were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to Smad9-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-AMH pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of AMH and Smad9 was shown in Figure 1, and this effect was in a dose dependent manner.)

Anti-Mullerian Hormone (AMH), Active Protein (Cat# AAA2097270)

Full Name
Active Anti-Mullerian Hormone (AMH)
Gene Names
AMH; MIF; MIS
Reactivity
Homo sapiens (Human)
Applications
Cell culture, Activity Assays.
Purity
>95%
Pricing
$250/0.01 mg | $540/0.05 mg | $840/0.1 mg | $1,045/0.2 mg | $2,040/0.5 mg | $3,020/1 mg | $7,385/5 mg
Western Blot (WB) (Western Blot: Sample: Rat Pancreas lysate; Primary Ab: 1ug/ml Rabbit Anti-Human Smad3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Mothers Against Decapentaplegic Homolog 3 (Smad3), Polyclonal Antibody (Cat# AAA2003140)

Full Name
Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 3 (Smad3)
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human
Applications
ICC, IHC, EIA, WB
Purity
Affinity Chromatography
Pricing
$165/0.1 mL | $205/0.2 mL | $345/0.5 mL | $425/1 mL | $1,220/5x1 mL

SMAD family member 5, ELISA Kit (Cat# AAA9330851)

Full Name
Human Mothers against decapentaplegic homolog 5, SMAD5 ELISA Kit
Gene Names
SMAD5; DWFC; JV5-1; MADH5
Reactivity
Human
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Western Blot (WB) (Western Blot: Sample: Human 293T cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Bovine Smad1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Mothers Against Decapentaplegic Homolog 1, Polyclonal Antibody (Cat# AAA2028248)

Full Name
Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1)
Reactivity
Cattle
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$170/0.1 mL | $215/0.2 mL | $370/0.5 mL | $455/1 mL | $1,280/5x1 mL
Western Blot (WB) (Western blot analysis of SP1 (pT453) expression in Jurkat (A), mouse melanoma cancer (B) whole cell lysates.)

SMAD2, Polyclonal Antibody (Cat# AAA8220276)

Full Name
Anti-SMAD2 (pS467) Phospho Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rabbit, Bovine, Chicken, Monkey, Sheep, Zebrafish, Rat, Pig
Applications
EIA, WB, IHC, IF, ICC
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$185/0.03 mL | $275/0.1 mL | $380/0.2 mL | $1,650/5x0.2 mL
Western Blot (WB) (Western blot analysis of SMAD3 (pS204) expression in HeLa UV-treated (A), SP2/0 UV-treated (B), H9C2 UV-treated (C) whole cell lysates.)

SMAD3 (pS204), Polyclonal Antibody (Cat# AAA821830)

Full Name
Anti-SMAD3 (pS204) Phospho Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat, Chicken, Monkey, Pig, Sheep
Applications
WB, IF, ICC, IP
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$185/0.03 mL | $275/0.1 mL | $380/0.2 mL | $1,650/5x0.2 mL
Western Blot (WB) (Western Blot analysis of various cells using Smad1/5/9 Polyclonal Antibody at dilution of 1:500.)

Smad1/5/9, Polyclonal Antibody (Cat# AAA2538175)

Full Name
Smad1/5/9 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from Hela cell,untreated or treated with PMA,using phospho-SMAD2-T220(left) or SMAD2 antibody(right).)

Phospho-SMAD2 (T220), Polyclonal Antibody (Cat# AAA9204628)

Full Name
Phospho-SMAD2 (T220) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human (Predicted Reactivity: Bovine, Zebrafish, Mouse, Rat)
Applications
IF, DB, EIA, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using SMAD3 Polyclonal Antibody at dilution 1:30)

SMAD3, Polyclonal Antibody (Cat# AAA2516444)

Full Name
SMAD3 Polyclonal Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC
Purity
Antigen affinity purification
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Western Blot (WB) (Western Blot analysis of A549 cells using Smad3 Polyclonal Antibody at dilution of 1:600)

Smad3, Polyclonal Antibody (Cat# AAA2541745)

Full Name
Smad3 Polyclonal Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Rat
Applications
WB, IHC
Purity
Antigen Affinity Purification
Pricing
$120/0.02 mL | $370/0.2 mL | $245/0.12 mL | $165/0.06 mL | $1,665/5x0.2 mL
Western Blot (WB) (Western blotPositive WB detected in: hepG2 cellAll lanes: SMAD2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53,49 kDaObserved band size: 52 kDa)

SMAD2, Polyclonal Antibody (Cat# AAA7044710)

Full Name
SMAD2 Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human
Applications
EIA, WB, IHC, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB) (Western blot analysis of MRE11 expression in K562 (A), HeLa (B), Jurkat (C) whole cell lysates.)

SMAD4, Polyclonal Antibody (Cat# AAA822108)

Full Name
Anti-SMAD4 Antibody
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human, Mouse, Rat, Bovine, Monkey, Pig, Zebrafish
Applications
WB, IHC, IF, ICC, IP
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$175/0.03 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL
Western Blot (WB) (Western Blot analysis of Human kindey cancer tissue using SMAD3 Polyclonal Antibody at dilution of 1:400)

SMAD3, Polyclonal Antibody (Cat# AAA2520446)

Full Name
SMAD3 Polyclonal Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen affinity purification
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Typical Testing Data/Standard Curve (for reference only)

Smad3/MADH3, ELISA Kit (Cat# AAA2511046)

Full Name
Human Smad3/MADH3 (Mothers Against Decapentaplegic Homolog 3) ELISA Kit
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Western Blot (WB) (Western blot analysis of SMAD7 expression in HeLa (A), A431 (B), SP2/0 (C), PC12 (D) whole cell lysates.)

SMAD7, Polyclonal Antibody (Cat# AAA821041)

Full Name
Anti-SMAD7 Antibody
Gene Names
SMAD7; CRCS3; MADH7; MADH8
Reactivity
Human, Mouse, Rat, Pig
Applications
WB
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$175/0.03 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human melanoma cancer using MBS7043826 at dilution 1:100)

SMAD5, Polyclonal Antibody (Cat# AAA7043826)

Full Name
SMAD5 Antibody
Gene Names
SMAD5; DWFC; JV5-1; MADH5
Reactivity
Human
Applications
EIA, WB, IHC, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB) (Western blotAll lanes: SMAD3 antibody at 8 ug/mlLane 1: Jurkat whole cell lysateLane 2: A431 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 49,44,36,26 kDaObserved band size: 49kDa)

SMAD3, Polyclonal Antibody (Cat# AAA1499593)

Full Name
SMAD3 Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human
Applications
EIA, WB, IHC, IP, IF
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Western Blot (WB) (Western Blot analysis of various cells using Smad2/3 Polyclonal Antibody at dilution of 1:500.)

Smad2/3, Polyclonal Antibody (Cat# AAA2536856)

Full Name
Smad2/3 Polyclonal Antibody
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Typical Testing Data/Standard Curve (for reference only)

Smad3, ELISA Kit (Cat# AAA2516123)

Full Name
Rat Smad3 (Mothers Against Decapentaplegic Homolog 3) ELISA Kit
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Rat
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Western blot (WB) (Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with TGF-beta (100ng/ml, 30 min), using Phospho-SMAD4 Antibody(upper) or Beta-actin (lower).)

Phospho-SMAD4 (T277), Polyclonal Antibody (Cat# AAA9207554)

Full Name
Phospho-SMAD4 (T277) Antibody
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human, Mouse
Predicted Species Reactivity: Rat
Applications
EIA, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB) (Western Blot analysis of various cells using Smad2 Polyclonal Antibody at dilution of 1:500.)

Smad2, Polyclonal Antibody (Cat# AAA2538917)

Full Name
Smad2 Polyclonal Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Western Blot (WB) (Western Blot analysis of A549, K562 cells with Smad1 Polyclonal Antibody.)

Smad1, Polyclonal Antibody (Cat# AAA2534819)

Full Name
Smad1 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Western Blot (WB) (Western blotAll lanes: SMAD1 antibody at 4 ug/mlLane 1: HepG-2 whole cell lysateLane 2: Mouse skeletal muscle tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 53,16 kDaObserved band size: 47 kDa)

SMAD1, Polyclonal Antibody (Cat# AAA7004305)

Full Name
SMAD1 Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Affinity Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB) (Western Blot analysis of various cells with Smad2 Polyclonal Antibody.)

Smad2, Polyclonal Antibody (Cat# AAA2536165)

Full Name
Smad2 Polyclonal Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Antigen Affinity Purified
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Western Blot (WB) (Western blotAll lanes : SMAD4 antibody at 12 ug/mlLane 1 : SH-SY5Y whole cell lysateLane 2 : 3T3 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 60 kDaObserved band size: 60,30 kDa)

SMAD4, Polyclonal Antibody (Cat# AAA7004264)

Full Name
SMAD4 Antibody
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
EIA, WB, IHC
Purity
>95%,Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using SMAD6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)

SMAD6, Polyclonal Antibody (Cat# AAA125675)

Full Name
SMAD6 Polyclonal Antibody
Gene Names
SMAD6; AOVD2; MADH6; MADH7; HsT17432
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunofluorescence (IF) (Fluorescent confocal image of HeLa cells stained with phospho-SMAD3-S208 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9213657 phospho-SMAD3-S208 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence).Note the highly specific localization of the phospho-SMAD3 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000166949).)

Phospho-SMAD3 (S208), Polyclonal Antibody (Cat# AAA9213657)

Full Name
Phospho-SMAD3 (S208) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
Applications
IF, DB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunofluorescence (IF) (MBS9601135 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Smad3, Polyclonal Antibody (Cat# AAA9601135)

Full Name
Phospho-Smad3 (Ser213) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of SW480 cells, using SMAD7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

Smad7, Polyclonal Antibody (Cat# AAA125498)

Full Name
Smad7
Gene Names
SMAD7; CRCS3; MADH7; MADH8
Reactivity
Human
Applications
WB, IF
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using Smad5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Smad5, Polyclonal Antibody (Cat# AAA9134033)

Full Name
Smad5 Polyclonal Antibody
Gene Names
SMAD5; DWFC; JV5-1; MADH5
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot detection of Smad2 in Hela, A431, Jurkat and K562 cell lysates using Smad2 mouse mAb (1:500 diluted).Predicted band size:60KDa.Observed band size:60KDa.)

Smad2, Monoclonal Antibody (Cat# AAA475095)

Full Name
Anti-Smad2 Mouse mAb
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human
Applications
WB, ICC
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Immunohistochemistry (IHC) (MBS9601132 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Smad3, Polyclonal Antibody (Cat# AAA9601132)

Full Name
Phospho-Smad3 (Thr179) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of HeLa cells treated with UV, using Phospho-Smad1 (Ser206) Antibody.)

Smad1, Polyclonal Antibody (Cat# AAA9600737)

Full Name
Phospho-Smad1 (Ser206) Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Immunofluorescence (IF) (MBS9601218 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Smad2, Polyclonal Antibody (Cat# AAA9601218)

Full Name
Phospho-Smad2 (Ser467) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of SMAD2 expression in HepG2 (A), HCC827 (B), HEK293T (C), mouse heart (D), rat heart (E) whole cell lysates.)

SMAD2, Polyclonal Antibody (Cat# AAA8242203)

Full Name
Anti-SMAD2 Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Monkey, Pig, Rabbit, Sheep, Zebrafish
Applications
WB, IHC, IF, ICC
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$175/0.03 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various tissue sample, using smad4 Antibody.)

Smad4, Polyclonal Antibody (Cat# AAA9601420)

Full Name
Smad4 Antibody
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of HeLa cells, using Phospho-Smad2-S465/467/-Smad3-S423/425 antibody at 1:1000 dilution. HeLa cells were treated by TGF-β (10ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 60s.)

Phospho-Smad2-S465/467, Antibody (Cat# AAA9129001)

Full Name
Phospho-Smad2-S465/467 Polyclonal Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB) (SMAD3 Antibody (S208) western blot analysis in HepG2 and mouse C2C12 cell line lysates (35ug/lane).This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).)

SMAD3-S208, Polyclonal Antibody (Cat# AAA9205803)

Full Name
SMAD3-S208 Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, mouse (Predicted Reactivity: Chicken, Pig, Rat)
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB) (Western blot analysis of Smad2/3 expression in UV treated COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Smad2/3, Polyclonal Antibody (Cat# AAA9601931)

Full Name
Smad2/3 Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of Smad7 expression in Human Kidney lysate, The lane on the left is treated with the antigen-specific peptide.)

Smad7, Polyclonal Antibody (Cat# AAA9601320)

Full Name
Smad7 Antibody
Gene Names
SMAD7; CRCS3; MADH7; MADH8
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9601221 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Smad1, Polyclonal Antibody (Cat# AAA9601221)

Full Name
Phospho-Smad1 (Ser187) Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using SMAD9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

SMAD9, Polyclonal Antibody (Cat# AAA9125825)

Full Name
SMAD9
Gene Names
SMAD9; PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Pricing
$225/0.05 mL | $305/0.1 mL | $465/0.2 mL
Quality Control (Western blot analysis of immunized recombinant protein, using anti-SMAD1 monoclonal antibody. )

SMAD1, Monoclonal Antibody (Cat# AAA120248)

Full Name
Mouse monoclonal antibody Anti-Human SMAD1
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human
Applications
DB, ICC, IP, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts of various cell lines,using SMAD1 antibody.)

SMAD1, Polyclonal Antibody (Cat# AAA2527072)

Full Name
SMAD1 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Antigen affinity purification
Pricing
$390/0.2 mL | $245/0.12 mL | $170/0.06 mL | $1,705/5x0.2 mL
Western Blot (WB) (Western blot analysis of Smad1/9 in lysates of Jurkat , using Smad1/9 Antibody(MBS9603223).)

Smad1/9, Polyclonal Antibody (Cat# AAA9603223)

Full Name
Smad1/9 Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL

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