Western Blot (WB)
(Western blot analysis of extracts from Hela cell,untreated or treated with PMA,using phospho-SMAD2-T220(left) or SMAD2 antibody(right).)
Rabbit Phospho-SMAD2 (T220) Polyclonal Antibody | anti-SMAD2 antibody
Phospho-SMAD2 (T220) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human (Predicted Reactivity: Bovine, Zebrafish, Mouse, Rat)
Applications
Immunofluorescence, Dot Blot, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-SMAD2 (T220); Polyclonal Antibody; Phospho-SMAD2 (T220) Antibody; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2; anti-SMAD2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Zebrafish, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SMAD2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T220 of human SMAD2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
437
Applicable Applications for anti-SMAD2 antibody
Immunofluorescence (IF), Dot Blot (DB), ELISA (EIA), Western Blot (WB)
Application Notes
WB~~1:500
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts from Hela cell,untreated or treated with PMA,using phospho-SMAD2-T220(left) or SMAD2 antibody(right).)
Western Blot (WB)
(Western blot analysis of extracts from Hela cell,untreated or treated with PMA,using phospho-SMAD2-T220(left) or SMAD2 antibody(right).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of Phospho-SMAD2-T220 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of Phospho-SMAD2-T220 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
Dot Blot (DB)
(Dot blot analysis of anti-Phospho-SMAD2-T220 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Dot Blot (DB)
(Dot blot analysis of anti-Phospho-SMAD2-T220 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Related Product Information for anti-SMAD2 antibody
SMAD2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smad's, activin/TGF?receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smad's; and the inhibitory Smad's, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling.
Product Categories/Family for anti-SMAD2 antibody
References
Papageorgis,P., et.al., Cancer Res. 70 (3), 968-978 (2010)
Funaba,M., et.al., J. Biol. Chem. 277 (44), 41361-41368 (2002)
Funaba,M., et.al., J. Biol. Chem. 277 (44), 41361-41368 (2002)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52306
NCBI Official Full Name
mothers against decapentaplegic homolog 2 isoform 1
NCBI Official Synonym Full Names
SMAD family member 2
NCBI Official Symbol
SMAD2
NCBI Official Synonym Symbols
JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
NCBI Protein Information
mothers against decapentaplegic homolog 2
UniProt Protein Name
Mothers against decapentaplegic homolog 2
Protein Family
UniProt Gene Name
SMAD2
UniProt Synonym Gene Names
MADH2; MADR2; MAD homolog 2; Mothers against DPP homolog 2; hMAD-2; SMAD 2; Smad2; hSMAD2
UniProt Entry Name
SMAD2_HUMAN
Similar Products
Product Notes
The SMAD2 smad2 (Catalog #AAA9204628) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-SMAD2 (T220) Antibody reacts with Human (Predicted Reactivity: Bovine, Zebrafish, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-SMAD2 (T220) can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Dot Blot (DB), ELISA (EIA), Western Blot (WB). WB~~1:500. Researchers should empirically determine the suitability of the SMAD2 smad2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-SMAD2 (T220), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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