Western Blot (WB)
(Western blot analysis of extracts of SW480 cells, using SMAD7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Rabbit anti-Human Smad7 Polyclonal Antibody | anti-SMAD7 antibody
Smad7
Gene Names
SMAD7; CRCS3; MADH7; MADH8
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
Affinity purification
Synonyms
Smad7; Polyclonal Antibody; SMAD7; CRCS3; MADH7; MADH8; SMAD family member 7; anti-SMAD7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
425
Applicable Applications for anti-SMAD7 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:50 - 1:1000
IF: 1:50 - 1:100
IF: 1:50 - 1:100
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 220-390 of human SMAD7 (NP_005895.1).
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Preparation and Storage
Store at -20°C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of SW480 cells, using SMAD7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of SW480 cells, using SMAD7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using SMAD7 antibody (MBS125498) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using SMAD7 antibody (MBS125498) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Related Product Information for anti-SMAD7 antibody
The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated Molecular Weight: 23 kDa / 46 kDa
Observed Molecular Weight: 46 kDa
Observed Molecular Weight: 46 kDa
NCBI Official Full Name
mothers against decapentaplegic homolog 7 isoform 2
NCBI Official Synonym Full Names
SMAD family member 7
NCBI Official Symbol
SMAD7
NCBI Official Synonym Symbols
CRCS3; MADH7; MADH8
NCBI Protein Information
mothers against decapentaplegic homolog 7; hSMAD7; MAD homolog 8; mothers against DPP homolog 8; SMAD, mothers against DPP homolog 7; Mothers against decapentaplegic, drosophila, homolog of, 7; MAD (mothers against decapentaplegic, Drosophila) homolog 7
UniProt Protein Name
Mothers against decapentaplegic homolog 7
Protein Family
UniProt Gene Name
SMAD7
UniProt Synonym Gene Names
MADH7; MADH8; MAD homolog 7; Mothers against DPP homolog 7; MAD homolog 8; Mothers against DPP homolog 8; SMAD 7; Smad7; hSMAD7
UniProt Entry Name
SMAD7_HUMAN
Similar Products
Product Notes
The SMAD7 smad7 (Catalog #AAA125498) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Smad7 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Smad7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:50 - 1:1000 IF: 1:50 - 1:100. Researchers should empirically determine the suitability of the SMAD7 smad7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smad7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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