Rabbit anti-Human SMAD3 Polyclonal Antibody | anti-SMAD3 antibody

SMAD3 Antibody

Cat. Num. AAA1499593

• Gene Names: SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
• Purity: Antigen Affinity Purified
• Synonyms: SMAD3; Polyclonal Antibody; SMAD3 Antibody; Mothers against decapentaplegic homolog 3 (MAD homolog 3) (Mad3) (Mothers against DPP homolog 3) (hMAD-3) (JV15-2) (SMAD family member 3) (SMAD3) (Smad3) (hSMAD3); MADH3; anti-SMAD3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid; PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Sequence Length: 320

• Applicable Applications for anti-SMAD3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescense (IF)
• Application Notes: Recommended dilution; WB: 1:1000-5000; IHC: 1:20-1:500; IF: 1:50-1:200; IP: 1:200-1:2000
• Conjugate: Non-conjugated
• Research Area: Signal Transduction
• Immunogen: Recombinant human Mothers against decapentaplegic homolog 3 protein (1-230aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-8332
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: SMAD3 antibody at 8 ug/mlLane 1: Jurkat whole cell lysateLane 2: A431 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 49,44,36,26 kDaObserved band size: 49kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using MBS1499593 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS1499593 at dilution 1:100)

Immunoprecipitation (IP)

(Immunoprecipitating SMAD3 in Jurkat whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1499593 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1499593(4ug)+ Jurkat whole cell lysate(500ug)Lane 3: Jurkat whole cell lysate (20ug))

Immunohistochemistry (IHC)

(IHC image of MBS1499593 diluted at1:388 and staining in paraffin-embedded humanadrenal gland tissue performed on a LeicaBondTM system. After dewaxing and hydration,antigen retrieval was mediated by high pressurein a citrate buffer (pH 6.0). Section was blockedwith 10% normal goat serum 30min at RT. Thenprimary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by abiotinylated secondary antibody and visualizedusing an HRP conjugated SP system.)

Immunofluorescence(IF)

(Immunofluorescence staining of A549 cells with MBS1499593 at 1:129, counter-stained with DAPI. The cells were fixed in 4%formaldehyde, permeabilized using 0.2% TritonX-100 and blocked in 10% normal Goat Serum.The cells were then incubated with the antibodyovernight at 4°C. The secondary antibody wasAlexa Fluor 488-congugated AffiniPure GoatAnti-Rabbit IgG(H+L).)

Related Product Information for anti-SMAD3 antibody

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

NCBI and Uniprot Product Information

• NCBI GI #: 223029440
• NCBI GeneID: 4088
• NCBI Accession #: NP_001138574.1
• NCBI GenBank Nucleotide #: NM_001145102.1
• UniProt Accession #: P84022; O09064; O09144; O14510; O35273; Q92940; Q93002; A8K4B6; B7Z4Z5; B7Z6M9; B7Z9Q2; F5H383
• NCBI Official Full Name: mothers against decapentaplegic homolog 3 isoform 2
• NCBI Official Synonym Full Names: SMAD family member 3
• NCBI Official Symbol: SMAD3
• NCBI Official Synonym Symbols: LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
• NCBI Protein Information: mothers against decapentaplegic homolog 3
• UniProt Protein Name: Mothers against decapentaplegic homolog 3
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: SMAD3
• UniProt Synonym Gene Names: MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3
• UniProt Entry Name: SMAD3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

Uniprot Description

SMAD3: transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

Protein type: Transcription factor; DNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 15q22.33

Cellular Component: cytoplasm; cytosol; nuclear chromatin; nuclear inner membrane; nucleoplasm; nucleus; plasma membrane; receptor complex; transcription factor complex

Molecular Function: beta-catenin binding; bHLH transcription factor binding; chromatin DNA binding; collagen binding; identical protein binding; phosphatase binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding; transforming growth factor beta receptor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; ubiquitin binding; ubiquitin protein ligase binding; zinc ion binding

Biological Process: activin receptor signaling pathway; caspase activation; cell cycle arrest; developmental growth; embryonic cranial skeleton morphogenesis; embryonic foregut morphogenesis; embryonic pattern specification; endoderm development; evasion of host defenses by virus; gene expression; heart looping; immune response; immune system development; in utero embryonic development; intercellular junction assembly and maintenance; liver development; mesoderm formation; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of fat cell differentiation; negative regulation of inflammatory response; negative regulation of mitotic cell cycle; negative regulation of osteoblast differentiation; negative regulation of osteoblast proliferation; negative regulation of protein amino acid phosphorylation; negative regulation of protein catabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; osteoblast development; paraxial mesoderm morphogenesis; pericardium development; positive regulation of bone mineralization; positive regulation of cell migration; positive regulation of chondrocyte differentiation; positive regulation of focal adhesion formation; positive regulation of interleukin-1 beta production; positive regulation of positive chemotaxis; positive regulation of stress fiber formation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor-beta3 production; primary microRNA processing; protein stabilization; regulation of binding; regulation of epithelial cell proliferation; regulation of immune response; regulation of striated muscle development; regulation of transcription from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; regulation of transforming growth factor-beta2 production; response to hypoxia; SMAD protein complex assembly; somitogenesis; T cell activation; thyroid gland development; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; transport; ureteric bud development; wound healing

Disease: Loeys-dietz Syndrome 3