Rabbit anti-Human, Rat Smad3 Polyclonal Antibody | anti-Smad3 antibody

Smad3 Polyclonal Antibody

Cat. Num. AAA2541745

• Gene Names: SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purification
• Synonyms: Smad3; Polyclonal Antibody; Smad3 Polyclonal Antibody; HSPC193; HST17436; LDS3; Mad protein homolog; MAD; MADH3; SMA and MAD related protein 3; SMAD family member 3; SMAD; mothers against DPP homolog 3; anti-Smad3 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purification
• Concentration: 4mg/ml (varies by lot)
• Sequence Length: 425

• Applicable Applications for anti-Smad3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:100
• Immunogen: Recombinant human Mothers against decapentaplegic homolog 3 protein
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-8332
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of A549 cells using Smad3 Polyclonal Antibody at dilution of 1:600)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Rat kidney using Smad3 Polyclonal Antibody at dilution of 1:50)

Related Product Information for anti-Smad3 antibody

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Product Categories/Family for anti-Smad3 antibody

Cancer; Epigenetics

NCBI and Uniprot Product Information

• NCBI GI #: 18418623
• NCBI GeneID: 4088
• Molecular Weight: Calculated MW: 48kD; Observed MW: 58kD
• NCBI Official Full Name: Smad3
• NCBI Official Synonym Full Names: SMAD family member 3
• NCBI Official Symbol: SMAD3
• NCBI Official Synonym Symbols: LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
• NCBI Protein Information: mothers against decapentaplegic homolog 3
• UniProt Protein Name: Mothers against decapentaplegic homolog 3
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: SMAD3
• UniProt Synonym Gene Names: MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3
• UniProt Entry Name: SMAD3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

Uniprot Description

SMAD3: transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 15q22.33

Cellular Component: cytoplasm; cytosol; nuclear chromatin; nuclear inner membrane; nucleoplasm; nucleus; receptor complex; transcription factor complex

Molecular Function: bHLH transcription factor binding; identical protein binding; phosphatase binding; protein binding; protein homodimerization activity; protein kinase binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding; transforming growth factor beta receptor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; ubiquitin binding; ubiquitin protein ligase binding; zinc ion binding

Biological Process: activin receptor signaling pathway; caspase activation; cell cycle arrest; evasion of host defenses by virus; immune response; intercellular junction assembly and maintenance; negative regulation of cell growth; negative regulation of fat cell differentiation; negative regulation of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of nitric oxide biosynthetic process; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; primary microRNA processing; reduction of cytosolic calcium ion concentration; regulation of transcription from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; regulation of transforming growth factor-beta2 production; response to hypoxia; SMAD protein complex assembly; transforming growth factor beta receptor signaling pathway; transport; wound healing

Disease: Loeys-dietz Syndrome 3