Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7001180 at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of HepG-2 cells using MBS7001180 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunohistochemistry (IHC) (MBS9602930 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of MINA using HepG2 whole cell lysates)
Western Blot (WB) (Western blot analysis of MINA in human heart tissue lysate with MINA antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of MINA in mouse heart tissue with MINA antibody at 5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of MINA in mouse heart tissue with MINA antibody at 20 μg/mL.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MINA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB) (Western blot analysis of anti-MINA (C-term) Pab pre-incubated with and without blocking peptide (BP) in Jurkat cell line lysate. MINA(C-term)(arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MINA (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB) (Western blot analysis of MINA (arrow) using rabbit polyclonal MINA Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MINA gene.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-C14orf169 monoclonal antibody. )
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Western Blot (WB) ((0.3ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB) (Western Blot showing MINA antibody used at a concentration of 1-2 ug/ml to detect its target protein.)
SDS-Page
Western Blot (WB) (MINA antibody (MBS835732) used at 0.2-1 ug/ml to detect target protein.)
Immunohistochemistry (IHC) (The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using C14orf169 Antibody at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: x200))
Western Blot (WB) (Western blot analysis of MINA53 expression in HEK293T (A), NIH3T3 (B), PC12 (C) whole cell lysates.)
Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human MYC-induced nuclear antigen (MINA) Standard Curve.)