Western Blot (WB)
((0.3ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat C14orf169/NO66 Polyclonal Antibody | anti-C14orf169 antibody
Goat anti-C14orf169/NO66 Antibody
Western Blot (WB)
((0.3ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
Uniprot Description
NO66: Histone demethylase that specifically demethylates 'Lys- 4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation. May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation. Belongs to the MINA53/NO66 family. NO66 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.11.27; Nucleolus
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: nucleolus; nucleoplasm; nucleus
Molecular Function: histone demethylase activity (H3-K36 specific); histone demethylase activity (H3-K4 specific); iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; protein binding
Biological Process: negative regulation of osteoblast differentiation; negative regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on C14orf169
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Product Notes
The C14orf169 no66 (Catalog #AAA421523) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-C14orf169/NO66 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's C14orf169/NO66 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Researchers should empirically determine the suitability of the C14orf169 no66 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GAQHSDSKDP R. It is sometimes possible for the material contained within the vial of "C14orf169/NO66, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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