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63 results for " Pharmacology" - showing 1-50


Immunofluorescence (IF) (Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9205943 cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

Cleaved LC3B, Polyclonal Antibody (Cat# AAA9205943)

Full Name
Cleaved LC3B Antibody
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse (Predicted Reactivity: Bovine)
Applications
IF, EIA, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF) ( Immunofluoresence staining of fixed MDA-MB-231 cells with anti-Glucose-dependent insulinotropic receptor antibody Gipg013 Immunofluorescence analysis of paraformaldehyde fixed MDA-MB-231 cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of Gipg013 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining with additional puncta. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)

Glucose-Dependent Insulinotropic Receptor, Monoclonal Recombinant Antibody (Cat# AAA488313)

Full Name
Anti-Glucose-Dependent Insulinotropic Receptor [Gipg013]
Gene Names
GPR119; GPCR2
Reactivity
Human, Rat, Dog, Mouse
Applications
EIA, BL, IF
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB) (Figure 1. Western blot analysis of HBD using anti-HBD antibody (MBS1750559).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: rat spleen tissue lysates,Lane 3: mouse spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HBD antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HBD at approximately 16KD. The expected band size for HBD is at 16KD. )

HBD, Polyclonal Antibody (Cat# AAA1750559)

Full Name
Anti-HBD Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg

Glucose-Dependent Insulinotropic Receptor, Monoclonal Recombinant Antibody (Cat# AAA488312)

Full Name
Anti-Glucose-Dependent Insulinotropic Receptor [Gipg013]
Gene Names
GPR119; GPCR2
Reactivity
Human, Rat, Dog, Mouse
Applications
EIA, BL, IF
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF) ( Immunofluorescence staining of fixed HepG2 cells with anti-A2A-Adenosine Receptor antibody Ig2838 (Ab00387) Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Ig2838 (Ab00387-1.1) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing granular cytoplasmic staining, possibly of protein in trafficking vesicles. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00387-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (Ab00178-1.1) followed by Alexa Fluor® 488 secondary antibody.)

A2A-Adenosine Receptor, Monoclonal Recombinant Antibody (Cat# AAA488308)

Full Name
Anti-A2A-Adenosine Receptor [Ig2838]
Gene Names
ADORA2A; A2aR; RDC8; ADORA2
Reactivity
Human
Applications
EIA, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg

P-glycoprotein, Recombinant Antibody (Cat# AAA488127)

Full Name
Anti-P-glycoprotein [MRK16]
Gene Names
ABCB1; CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170
Reactivity
Human
Applications
FC/FACS, IF, IP
Purity
Protein A Affinity Purified
Pricing
$635/0.1 mg | $2,660/5x0.1 mg
Immunohistochemistry (IHC) (Sample Type :Rhesus macaque spinal cordPrimary Antibody Dilution :1:300Secondary Antibody :Donkey anti Rabbit 488Secondary Antibody Dilution :1:500Color/Signal Descriptions :Green: BDNFGene Name :BDNFSubmitted by :Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706)

BDNF, Polyclonal Antibody (Cat# AAA3206141)

Full Name
BDNF antibody - middle region
Gene Names
BDNF; ANON2; BULN2
Reactivity
Dog, Horse, Human, Mouse, Pig, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunohistochemistry (IHC) (Rabbit Anti-KCNN3 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Adult heartObserved Staining: MembranePrimary Antibody Concentration: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy2/3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 secProtocol located in Reviews and Data.)

KCNN3, Polyclonal Antibody (Cat# AAA3202428)

Full Name
KCNN3 antibody - C-terminal region
Gene Names
KCNN3; SK3; hSK3; SKCA3; KCa2.3
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
WB, IHC
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunofluorescence (IF) ( Immunofluorescence staining of fixed HepG2 cells with anti-Human Glucagon Receptor antibody hGR-2 F6 (Ab00416) Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of hGR-2 F6 (Ab00416-1.1) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing punctate membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00416-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (Ab00178-1.1) followed by Alexa Fluor® 488 secondary antibody.)

Glucagon Receptor, Monoclonal Recombinant Antibody (Cat# AAA488309)

Full Name
Anti-Human Glucagon Receptor [hGR-2 F6]
Gene Names
GCGR; GGR; GL-R
Reactivity
Human
Applications
WB, EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS) (Flow-cytometry using the anti-P-glycoprotein antibody MRK16 (Ab00143) HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of MRK16 (Ab00143-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)

P-glycoprotein, Recombinant Antibody (Cat# AAA488128)

Full Name
Anti-P-glycoprotein [MRK16]
Gene Names
ABCB1; CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170
Reactivity
Human
Applications
FC/FACS, IF, IP
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC) (Rabbit Anti-ACTN2 antibodyFormalin Fixed Paraffin Embedded Tissue: Human Adult HeartObserved Staining: Cytoplasm in hepatocytesPrimary Antibody Concentration: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

ACTN2, Polyclonal Antibody (Cat# AAA3205863)

Full Name
ACTN2 antibody - N-terminal region
Gene Names
ACTN2; CMH23; CMD1AA
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunohistochemistry (IHC) (Sample Type :ACTNX-GFP transfected COS-7 cellsPrimary Antibody Dilution :1:1000Secondary Antibody :Anti-rabbit-Alexa-Fluor 568Secondary Antibody Dilution :1:100Color/Signal Descriptions :Green: GFP Red: ACTN1Gene Name :ACTN1Submitted by :Johannes W. Hell, Ph.D., Professor, Department of Pharmacology, University of California, Room 2419D Tupper Hall, Davis, CA 95616-5270, Phone: (530) 752 6540, Lab: (530) 752 7551, FAX: (530) 752 7710)

ACTN1, Polyclonal Antibody (Cat# AAA3213876)

Full Name
ACTN1 antibody - N-terminal region
Gene Names
ACTN1; BDPLT15
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunohistochemistry (IHC) (Sample Type :Rhesus macaque spinal cordPrimary Antibody Dilution :1:300Secondary Antibody :Donkey anti Rabbit 488Secondary Antibody Dilution :1:500Color/Signal Descriptions :Green: KCNN2Gene Name :KCNN2Submitted by :Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706)

KCNN2, Polyclonal Antibody (Cat# AAA3202584)

Full Name
KCNN2 antibody - middle region
Gene Names
KCNN2; SK2; hSK2; SKCA2; KCa2.2; SKCa 2
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunohistochemistry (IHC) (Sample Type :ACTNX-GFP transfected COS-7 cellsPrimary Antibody Dilution :1:1000Secondary Antibody :Anti-rabbit-Alexa-Fluor 568Secondary Antibody Dilution :1:100Color/Signal Descriptions :Green: GFP Red: ACTN4Gene Name :ACTN4Submitted by :Johannes W. Hell, Ph.D., Professor, Department of Pharmacology, University of California, Room 2419D Tupper Hall, Davis, CA 95616-5270, Phone: (530) 752 6540, Lab: (530) 752 7551, FAX: (530) 752 7710)

ACTN4, Polyclonal Antibody (Cat# AAA3206238)

Full Name
ACTN4 antibody - N-terminal region
Gene Names
ACTN4; FSGS; FSGS1; ACTININ-4
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Protein A purified
Pricing
$390/0.1 mL | $1,750/5x0.1 mL
Immunohistochemistry (IHC) (Sample Type :Rhesus macaque spinal cordPrimary Antibody Dilution :1:300Secondary Antibody :Donkey anti Rabbit 488Secondary Antibody Dilution :1:500Color/Signal Descriptions :Green: ATP2A2Gene Name :APLP2Submitted by :Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706)

ATP2A2, Polyclonal Antibody (Cat# AAA3207667)

Full Name
ATP2A2 antibody - C-terminal region
Gene Names
ATP2A2; DD; DAR; ATP2B; SERCA2
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunohistochemistry (IHC) (Lanes:Rat brain sectionPrimary Antibody Dilution:1:500Secondary Antibody:Anti-rabbit-biotin, streptavidin-diaminobenzidineSecondary Antibody Dilution:1:500Gene Name:KCNN2Submitted by:Dr. Amiel Rosenkranz, Rosalind Franklin University)

KCNN2, Polyclonal Antibody (Cat# AAA3202426)

Full Name
KCNN2 antibody - C-terminal region
Gene Names
KCNN2; SK2; hSK2; SKCA2; KCa2.2; SKCa 2
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish, Monkey
Applications
IHC, WB
Purity
Protein A purified
Pricing
$390/0.1 mL | $1,750/5x0.1 mL
Western Blot (WB) (Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.)

ATG12, Polyclonal Antibody (Cat# AAA9203753)

Full Name
ATG12 Antibody (N-term)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human, mouse
Applications
WB, EIA, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.)

ATG12, Polyclonal Antibody (Cat# AAA648113)

Full Name
ATG12, NT (APG12, APG12L, HAPG12, APG12 Autophagy 12-like, APG12-like, Apg12 (Autophagy, yeast) Homolog, Autophagy-related Protein 12)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human, Mouse
Applications
EL/EIA, WB, IF
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Western Blot (WB) (Western Blot analysis of extracts from COS cells, untransfected (lane 1) or transfected with either wild-type (lanes 2 and 3) or Ala916 mutant (lanes 4 and 5) Ras-GRF1, using Phospho-Ras-GRF1 (Ser916) Antibody (upper) or Ras-GRF1 R1198-16 (lower). Transfected COS cells were untreated or treated forskolin as indicated.(Triple HA-tagged Ras-GRF1 expressing plasmids provided by Dr. R. Mattingly, Dept. of Pharmacology, Wayne State University, Michigan.))

Ras, GRF1, Polyclonal Antibody (Cat# AAA613565)

Full Name
Ras, GRF1, phosphorylated (Ser916)
Gene Names
RASGRF1; GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1
Reactivity
Mouse
Applications
WB
Purity
Affinity Purified
Purified by protein A and peptide affinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL

GABA B Receptor 2, Polyclonal Antibody (Cat# AAA541660)

Full Name
GABA B Receptor 2 Antibody BIOTIN-Conjugated
Reactivity
Cat, Human
Applications
EIA, IP, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg

GABA B Receptor 2, Polyclonal Antibody (Cat# AAA541661)

Full Name
GABA B Receptor 2 Antibody FITC-Conjugated
Reactivity
Cat, Human
Applications
EIA, IP, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts from guinea pig neutrophils stimulated with 1uM fMLP for indicated times, using Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody (Provided by Drs. Qian Zhan and John Badwey, Dept. of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Massachusetts.))

PAK1,2,3, Polyclonal Antibody (Cat# AAA620632)

Full Name
PAK1,2,3, phosphorylated (Thr423/Thr402/Thr421) (p21 Activated Kinase 1,2,3)
Reactivity
Guinea Pig, Human, Mouse, Rat
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts from COS cells cotransfected with activated cdc42 and GST-PAK2 (lane 1), transfected with GST-PAK2 alone (lane 2) or activated cdc42 alone (lane 3), using Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody (upper) or PAK1/2/3 Antibody #P2488-15.)

PAK1,2, Polyclonal Antibody (Cat# AAA620506)

Full Name
PAK1,2, phosphorylated (Ser199/204) (Ser192/197) (p21 Activated Kinase 1, 2)
Reactivity
Guinea Pig, Human, Mouse, Rat
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human kidney, using ARG1 Antibody(MBS179560)ARG1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ARG1 Antibody (MBS179560)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(MBS176451) with DAB as the chromogen. )

ARG1/Arginase 1, Monoclonal Antibody (Cat# AAA179560)

Full Name
Anti-ARG1/Arginase 1 Rabbit Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1806a cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

MAP1ALC3, cleaved, Polyclonal Antibody (Cat# AAA6010760)

Full Name
MAP1ALC3, cleaved (LC3B, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associat
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse
Applications
EL/EIA, ICC, IF
Purity
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Western Blot (WB) (KCTD8 Antibody (C-term) western blot analysis in mouse liver and heart tissue lysates (35ug/lane).This demonstrates the KCTD8 antibody detected the KCTD8 protein (arrow).)

KCTD8, Polyclonal Antibody (Cat# AAA9210853)

Full Name
KCTD8 Antibody (C-term)
Reactivity
Human, mouse
Applications
WB, EIA, IHC-p
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL

GABA B Receptor 2, Positive Control (Cat# AAA542782)

Full Name
GABA B Receptor 2 Positive Control
Applications
WB
Pricing
$335/5 Applications | $1,350/5x5 Applications

GABA B Receptor 2, Polyclonal Antibody (Cat# AAA541663)

Full Name
GABA B Receptor 2 Antibody
Reactivity
Cat, Human
Applications
EIA, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Immunohistochemistry (IHC)

GABA B Receptor 2, Polyclonal Antibody (Cat# AAA541662)

Full Name
GABA B Receptor 2 Antibody
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg

GABA B Receptor 2, Blocking Peptide (Cat# AAA543714)

Full Name
GABA B Receptor 2 Peptide
Applications
Immunodepletion, Immunocompetition
Pricing
$280/0.25 mg | $1,110/5x0.25 mg

GABA B Receptor 2, Polyclonal Antibody (Cat# AAA541659)

Full Name
GABA B Receptor 2 Antibody FITC-Conjugated
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, IP, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg

GABA B Receptor 2, Polyclonal Antibody (Cat# AAA541658)

Full Name
GABA B Receptor 2 Antibody BIOTIN-Conjugated
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, IP, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
Western Blot (WB) (Western blot analysis of Dystrophin expression in human fetal heart lysate (MBS179074).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DMD monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DMD )

Dystrophin, Monoclonal Antibody (Cat# AAA179074)

Full Name
Anti-Dystrophin Rabbit Monoclonal Antibody
Gene Names
DMD; BMD; CMD3B; MRX85; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL

GABA B Receptor 2, Blocking Peptide (Cat# AAA543713)

Full Name
GABA B Receptor 2 Peptide
Applications
Immunodepletion, Immunocompetition
Pricing
$280/0.25 mg | $1,110/5x0.25 mg
Testing Data (Fumalic acid(Ferulic acid) is a hydroxycinnamic acid and a type of organic compound found in the Ferula assafoetida L. or Ligusticum chuanxiong. It has many pharmacology functions, such as anti-blood platelet collection, ease pain better the alive of prostate, relax the blood vessel convulsion. Like many phenols, it is an antioxidant in the sense that it is reactive toward free radicals such as reactive oxygen species (ROS). ROS and free radicals are implicated in DNA damage, cancer, accelerated cell aging. Animal studies and in vitro studies suggest that ferulic acid may have direct antitumor activity against breast cancer and liver cancer. Ferulic acid may have pro-apoptotic effects in cancer cells, thereby leading to their destruction. Ferulic acid may be effective at preventing cancer induced by exposure to the carcinogenic compounds benzopyrene and 4-nitroquinoline 1-oxide. [1][2])

Fumalic acid, Inhibitor (Cat# AAA8506271)

Full Name
Fumalic acid (Ferulic acid)
Purity
>99%
Pricing
$295/50 mg | $1,255/5x50 mg
Immunohistochemistry (IHC) (Sample Type :Rhesus macaque spinal cordPrimary Antibody Dilution :1:300Secondary Antibody :Donkey anti Rabbit 488Secondary Antibody Dilution :1:500Color/Signal Descriptions :Green: ATP2A1Gene Name :ATP2A1Submitted by :Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706)

ATP2A1, Polyclonal Antibody (Cat# AAA3206230)

Full Name
ATP2A1 antibody - N-terminal region
Gene Names
ATP2A1; ATP2A; SERCA1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human liver, using Liver Arginase Antibody(MBS179561)ARG1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ARG1 Antibody (MBS179561)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

Liver Arginase, Monoclonal Antibody (Cat# AAA179561)

Full Name
Anti-Liver Arginase Rabbit Monoclonal Antibody
Reactivity
Human
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.)

ATG12, Polyclonal Antibody (Cat# AAA6463468)

Full Name
ATG12, NT (APG12, APG12L, HAPG12, APG12 Autophagy 12-like, APG12-like, Apg12 (Autophagy, yeast) Homolog, Autophagy-related Protein 12) (APC)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human, Mouse
Applications
IF, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunohistochemistry (IHC) (Sample Type : Mouse sciatic nervePrimary Antibody Dilution : 1:500Secondary Antibody : Biotinylated Anti-Rabbit 1:1000 followed by avidin-biotin and diaminobenzidineSecondary Antibody Dilution : 1:1000Gene Name : MFAP4 Submitted by :Beth Friedman, Ph.D., Project Scientist, Department of Pharmacology, UCSD School of Medicine, San Diego, CA)

MFAP4, Polyclonal Antibody (Cat# AAA3205886)

Full Name
MFAP4 antibody - N-terminal region
Reactivity
Tested Species Reactivity: Human, Mouse
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunohistochemistry (IHC) (Sample Type :Rhesus macaque spinal cordPrimary Antibody Dilution :1:300Secondary Antibody :Donkey anti Rabbit 488Secondary Antibody Dilution :1:500Color/Signal Descriptions :Green: SPSB2Gene Name :SPSB2Submitted by :Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706)

SPSB2, Polyclonal Antibody (Cat# AAA3214869)

Full Name
SPSB2 antibody - C-terminal region
Gene Names
SPSB2; SSB2; GRCC9
Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB) (Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.)

ATG12, Polyclonal Antibody (Cat# AAA6463467)

Full Name
ATG12, NT (APG12, APG12L, HAPG12, APG12 Autophagy 12-like, APG12-like, Apg12 (Autophagy, yeast) Homolog, Autophagy-related Protein 12) (AP)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunohistochemistry (IHC) (Sample Type :Rhesus macaque spinal cordPrimary Antibody Dilution :1:300Secondary Antibody :Donkey anti Rabbit 488Secondary Antibody Dilution :1:500Color/Signal Descriptions :Green: SPSB2Gene Name :SPSB2Submitted by :Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706)

SPSB2, Polyclonal Antibody (Cat# AAA3214868)

Full Name
SPSB2 antibody - N-terminal region
Gene Names
SPSB2; SSB2; GRCC9
Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB) (Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.)

ATG12, Polyclonal Antibody (Cat# AAA6463470)

Full Name
ATG12, NT (APG12, APG12L, HAPG12, APG12 Autophagy 12-like, APG12-like, Apg12 (Autophagy, yeast) Homolog, Autophagy-related Protein 12) (FITC)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human, Mouse
Applications
IF, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1806a cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

MAP1ALC3, Polyclonal Antibody (Cat# AAA6318255)

Full Name
MAP1ALC3, cleaved (LC3B, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associat
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse
Applications
EIA, IF, ICC
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1806a cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

MAP1ALC3, Polyclonal Antibody (Cat# AAA6318258)

Full Name
MAP1ALC3, cleaved (LC3B, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associat
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse
Applications
IF, ICC
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Western Blot (WB) (Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.)

ATG12, Polyclonal Antibody (Cat# AAA6463471)

Full Name
ATG12, NT (APG12, APG12L, HAPG12, APG12 Autophagy 12-like, APG12-like, Apg12 (Autophagy, yeast) Homolog, Autophagy-related Protein 12) (Azide free) (HRP)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB) (Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.)

ATG12, Polyclonal Antibody (Cat# AAA6463469)

Full Name
ATG12, NT (APG12, APG12L, HAPG12, APG12 Autophagy 12-like, APG12-like, Apg12 (Autophagy, yeast) Homolog, Autophagy-related Protein 12) (Biotin)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human, Mouse
Applications
IF, EIA, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1806a cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

MAP1ALC3, Polyclonal Antibody (Cat# AAA6318254)

Full Name
MAP1ALC3, cleaved (LC3B, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associat
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse
Applications
EIA, IF, ICC
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1806a cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

MAP1ALC3, Polyclonal Antibody (Cat# AAA6318262)

Full Name
MAP1ALC3, cleaved (LC3B, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associat
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse
Applications
IF, ICC
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1806a cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

MAP1ALC3, Polyclonal Antibody (Cat# AAA6318261)

Full Name
MAP1ALC3, cleaved (LC3B, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associat
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse
Applications
IF, ICC
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
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