Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant DHFR protein (Right) or empty vector (Left) detected with DHFR antibody)
Mouse DHFR Monoclonal Antibody | anti-DHFR antibody
DHFR antibody
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
DHFR antibody was purified by affinity chromatography.
Synonyms
DHFR; Monoclonal Antibody; DHFR antibody; Monoclonal DHFR; Anti-DHFR; Dihydrofolate reductase; DHFRP1; anti-DHFR antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6G7
Purity/Purification
DHFR antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
173
Applicable Applications for anti-DHFR antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100
IHC: 1:50
WB: 1:2000
IHC: 1:50
WB: 1:2000
Biological Significance
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novosynthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene hasbeen mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
Cross-Reactivity
Human, Monkey, Dog
Immunogen
DHFR antibody was raised in mouse using a protein expressed in 293T cell transfected with human DHFR expression vector as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant DHFR protein (Right) or empty vector (Left) detected with DHFR antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant DHFR protein (Right) or empty vector (Left) detected with DHFR antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of DHFR protein in paraffin embedded Carcinoma of Human kidney tissue using DHFR antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of DHFR protein in paraffin embedded Carcinoma of Human kidney tissue using DHFR antibody)
Related Product Information for anti-DHFR antibody
Mouse monoclonal DHFR antibody
Product Categories/Family for anti-DHFR antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The DHFR (Catalog #AAA831788) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DHFR can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100 IHC: 1:50 WB: 1:2000. Researchers should empirically determine the suitability of the DHFR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHFR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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