Rabbit KCNN3 Polyclonal Antibody | anti-KCNN3 antibody
KCNN3 antibody - C-terminal region
Immunohistochemistry (IHC)
(Rabbit Anti-KCNN3 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Adult heartObserved Staining: MembranePrimary Antibody Concentration: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy2/3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 secProtocol located in Reviews and Data.)
Immunohistochemistry (IHC)
(Sample Type :Rhesus macaque spinal cordPrimary Antibody Dilution :1:300Secondary Antibody :Donkey anti Rabbit 488Secondary Antibody Dilution :1:500Color/Signal Descriptions :Green: KCNN3Gene Name :KCNN3Submitted by :Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706)
Western Blot (WB)
(Host: RabbitTarget Name: KCNN3Sample Tissue: Human THP-1 Whole CellAntibody Dilution: 1ug/ml)
Western Blot (WB)
(KCNN3 antibody - C-terminal region validated by WB using Mouse kidney, Whole brain lysate at 2 ug/ml.)
Western Blot (WB)
(WB Suggested Anti-KCNN3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Daudi cell lysateKCNN3 is supported by BioGPS gene expression data to be expressed in Daudi)
Target Description: Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. KCNN3, a member of the KCNN family of potassium channel genes, encodes a protein that is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits.
NCBI and Uniprot Product Information
NCBI Description
Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. This gene belongs to the KCNN family of potassium channels. It encodes an integral membrane protein that forms a voltage-independent calcium-activated channel, which is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. This gene contains two CAG repeat regions in the coding sequence. It was thought that expansion of one or both of these repeats could lead to an increased susceptibility to schizophrenia or bipolar disorder, but studies indicate that this is probably not the case. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
Uniprot Description
Function: Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.
Subunit structure: Heterooligomer. The complex is composed of 4 channel subunits each of which binds to a calmodulin subunit which regulates the channel activity through calcium-binding
By similarity.
Subcellular location: Membrane; Multi-pass membrane protein.
Polymorphism: The second poly-Gln region of KCNN3 is highly polymorphic and the number of Gln varies from 12 to 28 in the population.
Sequence similarities: Belongs to the potassium channel KCNN family. KCa2.3/KCNN3 subfamily.
