$405/0.1 mL (Concentrate) | $695/0.5 mL (Concentrate) | $1,075/1 mL (Concentrate) | $555/0.2 mL (Concentrate) | $205/0.04 mL (Concentrate) | $4,575/5x1 mL (Concentrate)
Immunofluorescence (IF) (MBS9600137 at 1/100 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600 was used as secondary antibody.)
Immunohistochemistry (IHC) (MBS9600137 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis on NIH-3T3 cell lysate using iNOS Antibody. The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC) (MBS9600137 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of extracts from mouse brain, using iNOS Antibody.)
Immunohistochemistry (IHC) (MBS9600137 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of eNOS (pS1177) expression in A549 (A), HuvEc (B), rat brain (C) whole cell lysates.)
Immunofluorescence (IF) (Immunofluorescent analysis of eNOS (pS1177) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Western Blot (WB) (Western blot analysis of extracts from heat-shock treated RAW264.7 cells.7, using Phospho-iNOS (Tyr151) Antibody. The lane on the left was treated with blocking peptide.)
Immunohistochemistry (IHC) (MBS9601041 at 1/100 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of iNOS expression in NIH/3T3 cells.The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC) (MBS9601541 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunofluorescence (IF) (MBS9601541 staining Raw264.7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB) (Western blot analysis of eNOS mouse monoclonal antibody expression in HuvEc, Mouse vascular and Rat vascular cell/tissue lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eNOS(Phospho-Ser1177) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed Hela cells using eNOS(Phospho-Ser1177) Antibody.)
Western blot (Western blot analysis of extracts from 3T3 cells treated with EGF, HWEC cells treated with VEGF and JK cells using eNOS (Phospho-Ser1177) Antibody #MBS9414700.)
Western Blot (WB) (Western BlotPositive WB detected in:Mouse spleen tissue,Mouse lung tissue,Mouse kidney tissueAll lanes: NOS1 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 161,149,126,44,165 kDaObserved band size: 161,126,92 kDa)
Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells using MBS7049643 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blot analysis of iNOS expression in NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC) (MBS9601851 at 1/100 staining Human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)