Typical Testing Data/Standard Curve (for reference only)
Rat nitric oxide synthase 3 (endothelial cell) ELISA Kit | NOS3 elisa kit
Rat Endothelial nitric oxide synthase, eNOS/NOS3 ELISA Kit
Gene Names
Nos3; eNos
Reactivity
Rat
Synonyms
nitric oxide synthase 3 (endothelial cell); Rat Endothelial nitric oxide synthase; eNOS/NOS3 ELISA Kit; ECNOS; eNOS; nitric oxide synthase 3; NOS3 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat eNOS. No significant cross-reactivity or interference between rat eNOS and analogues was observed.
Sequence Length
1202
Samples
serum, plasma, cell culture supernates and cell lysates.
Assay Type
Sandwich (Quantitative)
Detection Range
15.6 uIU/ml -1000 uIU/ml.
Sensitivity
Typically less than 3.9 uIU/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
133,290 Da
NCBI Official Full Name
nitric oxide synthase, endothelial
NCBI Official Synonym Full Names
nitric oxide synthase 3, endothelial cell
NCBI Official Symbol
Nos3
NCBI Official Synonym Symbols
eNos
NCBI Protein Information
nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; endothelial nitric oxide synthase 3
UniProt Protein Name
Nitric oxide synthase, endothelial
Protein Family
UniProt Gene Name
Nos3
UniProt Synonym Gene Names
cNOS; eNOS; NOSIII
UniProt Entry Name
NOS3_RAT
NCBI Description
enzyme that synthesizes Nitric oxide from L-arginine [RGD, Feb 2006]
Uniprot Description
Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.
Cofactor: Heme group.
Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN
Research Articles on NOS3
Similar Products
Product Notes
The Rat NOS3 nos3 (Catalog #AAA702741) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA702741 ELISA Kit recognizes Rat NOS3. It is sometimes possible for the material contained within the vial of "nitric oxide synthase 3 (endothelial cell), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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