Human nitric oxide synthase 3 (endothelial cell) ELISA Kit | NOS3 elisa kit

Human Endothelial nitric oxide synthase, eNOS ELISA Kit

Cat. Num. AAA701218

• Gene Names: NOS3; eNOS; ECNOS
• Reactivity: Human
• Synonyms: nitric oxide synthase 3 (endothelial cell); Human Endothelial nitric oxide synthase; eNOS ELISA Kit; ECNOS; eNOS; nitric oxide synthase 3; NOS3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human NOS3. No significant cross-reactivity or interference between Human NOS3 and analogues was observed.
• Sequence Length: 1203

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 1.1 IU/ml-70 IU/ml
• Sensitivity: 0.27 IU/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOS3 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for eNOS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any eNOS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for eNOS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of eNOS bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 40254422
• NCBI GeneID: 4846
• NCBI Accession #: NP_000594.2
• NCBI GenBank Nucleotide #: NM_000603.4
• UniProt Accession #: P29474; Q13662; Q14251; Q14434; Q548C1; Q6GSL5; Q9UDC6; A0S0A7; A0S0A8; A8KA63; B2RCQ1; E9PFR2
• Molecular Weight: 133,289 Da
• NCBI Official Full Name: nitric oxide synthase, endothelial isoform 1
• NCBI Official Synonym Full Names: nitric oxide synthase 3 (endothelial cell)
• NCBI Official Symbol: NOS3
• NCBI Official Synonym Symbols: eNOS; ECNOS
• NCBI Protein Information: nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3 transcript variant eNOS-delta20-21
• UniProt Protein Name: Nitric oxide synthase, endothelial
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS3
• UniProt Synonym Gene Names: cNOS; eNOS; NOSIII
• UniProt Entry Name: NOS3_HUMAN

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.1 PublicationManual assertion based on experiment in:Ref.6

Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor: Heme group.

Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.2 PublicationsManual assertion based on experiment in:Ref.19