Mouse nitric oxide synthase 3 (endothelial cell) ELISA Kit | NOS3 elisa kit

Mouse Endothelial nitric oxide synthase, eNOS ELISA Kit

Cat. Num. AAA700721

• Gene Names: Nos3; eNOS; Nos-3; ecNOS; 2310065A03Rik
• Reactivity: Mouse
• Synonyms: nitric oxide synthase 3 (endothelial cell); Mouse Endothelial nitric oxide synthase; eNOS ELISA Kit; ECNOS; eNOS; nitric oxide synthase 3; NOS3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse NOS3. No significant cross-reactivity or interference between Mouse NOS3 and analogues was observed.
• Sequence Length: 1202

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 0.312 IU/ml-20 IU/ml
• Sensitivity: 0.078 IU/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOS3 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to eNOS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for eNOS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain eNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of eNOS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 161086988
• NCBI GeneID: 18127
• NCBI Accession #: NP_032739.3
• NCBI GenBank Nucleotide #: NM_008713.4
• UniProt Accession #: P70313; O55056; Q7TSV7
• Molecular Weight: 132,916 Da
• NCBI Official Full Name: nitric oxide synthase, endothelial
• NCBI Official Synonym Full Names: nitric oxide synthase 3, endothelial cell
• NCBI Official Symbol: Nos3
• NCBI Official Synonym Symbols: eNOS; Nos-3; ecNOS; 2310065A03Rik
• NCBI Protein Information: nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; endothelial nitric oxide synthase
• UniProt Protein Name: Nitric oxide synthase, endothelial
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: Nos3
• UniProt Synonym Gene Names: Ecnos; cNOS; eNOS; NOSIII
• UniProt Entry Name: NOS3_MOUSE

Uniprot Description

Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.1 PublicationManual assertion based on experiment in:Ref.3

Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor: Heme group.

Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN