SCN3B recombinant protein

Recombinant Human SCN3B Protein (His tag)

Cat. Num. AAA2545300

• Gene Names: SCN3B; SCNB3; ATFB16; BRGDA7; HSA243396
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: SCN3B; Recombinant Human SCN3B Protein (His tag); KIAA1158; SCN3B recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 215

• Application Notes: The recombinant human SCN3B consists of 148 amino acids and predicts a molecular mass of 17.2 KDa. It migrates as an approximately 29 and 34 kDa due to different glycosylation.
• Predicted N Terminal: Phe 23
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SCN3B recombinant protein

Background: SCN3B (sodium channel, voltage-gated, type III, beta, human IgG1-Fc chimera) belongs to the sodium channel auxiliary subunit SCN3B family. It contains 1 Ig-like C2-type (immunoglobulin-like) domain. Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. SCN3B gene encodes one member of the sodium channel beta subunit gene family, and influences the inactivation kinetics of the sodium channel. Two alternatively spliced variants, encoding the same protein, have been identified. Defects in SCN3B are the cause of Brugada syndrome type 7. A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram. It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset.

Description: A DNA sequence encoding the mature form of human SCN3B (Q9NY72) extracellular domain (Met1-Glu159) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 93587332
• NCBI GeneID: 55800
• NCBI Accession #: NP_001035241.1
• NCBI GenBank Nucleotide #: NM_001040151.1
• UniProt Accession #: Q9NY72; Q17RL3; Q9ULR2; A5H1I5
• Molecular Weight: 24,702 Da
• NCBI Official Full Name: sodium channel subunit beta-3
• NCBI Official Synonym Full Names: sodium voltage-gated channel beta subunit 3
• NCBI Official Symbol: SCN3B
• NCBI Official Synonym Symbols: SCNB3; ATFB16; BRGDA7; HSA243396
• NCBI Protein Information: sodium channel subunit beta-3
• UniProt Protein Name: Sodium channel subunit beta-3
• Protein Family: Sodium channel
• UniProt Gene Name: SCN3B
• UniProt Synonym Gene Names: KIAA1158

NCBI Description

Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel beta subunit gene family, and influences the inactivation kinetics of the sodium channel. Two alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

SCN3B: Modulates channel gating kinetics. Causes unique persistent sodium currents. Inactivates the sodium channel opening more slowly than the subunit beta-1. Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons. Defects in SCN3B are the cause of Brugada syndrome type 7 (BRGDA7). A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the sodium channel auxiliary subunit SCN3B (TC 8.A.17) family.

Protein type: Channel, sodium; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q24.1

Cellular Component: plasma membrane; voltage-gated sodium channel complex; Z disc

Molecular Function: sodium channel regulator activity

Biological Process: cardiac muscle contraction; membrane depolarization; positive regulation of heart rate

Disease: Brugada Syndrome 7