Rabbit anti-Human RAD52 Polyclonal Antibody | anti-RAD52 antibody

RAD52, ID (RAD52, DNA repair protein RAD52 homolog) (AP)

Cat. Num. AAA6340042

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RAD52; Polyclonal Antibody; ID (RAD52; DNA repair protein RAD52 homolog) (AP); DNA repair protein RAD52 homolog; anti-RAD52 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RAD52 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RAD52 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-205 amino acids from the Central region of human RAD52.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RAD52 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RAD52 antibody detected the RAD52 protein (arrow).)

Related Product Information for anti-RAD52 antibody

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair.

Product Categories/Family for anti-RAD52 antibody

Antibodies; DNA Replication; Repair

References

Liu, Y., et al. Carcinogenesis 31(10):1762-1769(2010). Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010). In press: Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010). Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Monsees, G.M., et al. Breast Cancer Res. Treat. (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 109637798
• NCBI GeneID: 5893
• NCBI Accession #: NP_602296.2
• NCBI GenBank Nucleotide #: NM_134424.2
• UniProt Accession #: P43351; Q13205
• Molecular Weight: 46,169 Da
• NCBI Official Full Name: DNA repair protein RAD52 homolog
• NCBI Official Synonym Full Names: RAD52 homolog (S. cerevisiae)
• NCBI Official Symbol: RAD52
• NCBI Protein Information: DNA repair protein RAD52 homolog; recombination protein RAD52; rhabdomyosarcoma antigen MU-RMS-40.23
• UniProt Protein Name: DNA repair protein RAD52 homolog
• Protein Family: RAD52 motif-containing protein
• UniProt Gene Name: RAD52
• UniProt Entry Name: RAD52_HUMAN

NCBI Description

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. [provided by RefSeq, Jul 2008]

Uniprot Description

RAD52: a protein involved in DNA double strand breaks and recombination. Associates with and is phosphorylated by c-Abl. Belongs to the RAD52/RAD22 family.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 12p13-p12.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding

Biological Process: DNA recombinase assembly; double-strand break repair; DNA repair; double-strand break repair via homologous recombination; DNA recombination